Low potential of fish as a source of infection with
Angiostrongylus cantonensis

J 2024

Low potential of fish as a source of infection with Angiostrongylus cantonensis

ANETTOVÁ, Lucia; Jana KAČMAŘÍKOVÁ; Anna ŠIPKOVÁ; Vivienne VELIČ; Lia CAVALLO et al.

Základní údaje

Originální název

Low potential of fish as a source of infection with Angiostrongylus cantonensis

Autoři

ANETTOVÁ, Lucia; Jana KAČMAŘÍKOVÁ; Anna ŠIPKOVÁ; Vivienne VELIČ; Lia CAVALLO; Iva DYKOVÁ; Elena IZQUIERDO-RODRIGUEZ; Eric R MORGAN a David MODRÝ

Vydání

Food Quality and Safety, Oxford University Press, 2024, 2399-1399

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10613 Zoology

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 4.400

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/24:00137227

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

Angiostrongylus cantonensis; rat lungworm; life cycle; fish; food-borne pathogen

Štítky

14314020, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 27. 1. 2025 07:53, Mgr. Marie Novosadová Šípková, DiS.

Anotace

V originále

Objectives: Fish are hypothesized to act as paratenic hosts for the zoonotic nematode Angiostrongylus cantonensis, which causes human eosinophilic meningitis.There is a lack of data confirming the relevance of fish in A. cantonensis life cycle and their contribution to human infection. Materials and Methods: We conducted a series of experiments to investigate the survival and infectivity of A. cantonensis larvae in Clarias gariepinus (catfish; n =30) and Oreochromis niloticus (tilapia; n =24). Each fish was inoculated with 10 000 third-stage larvae (L3). Larval survival was assessed through artificial digestion of fish tissues 1 week, 2 weeks, and 3 weeks post-infection. To investigate the early stages of infection, four catfish were inoculated with 10 000 L3 each and sacrificed 3 d post-infection. Quantitative polymerase chain reaction (qPCR) analysis and histopathological examination were performed to evaluate larval distribution and tissue reactions. Two infected catfish, sacrificed 1 week post-infection, were used to feed Wistar rats. Results: After 45 d, the rats did not shed first-stage larvae (L1), indicating the absence of infection. One week post-infection, dead larvae were present in the digested tissues of both fish species, and the same was observed 2 weeks and 3 weeks after exposure. qPCR analysis revealed that the intestine was the most heavily infected organ. Histopathological examination identified dead larvae within granulomas in both the testines and liver. Early-stage infection experiment showed that fish sacrificed 3 d post-inoculation contained viable L3, which was infective Wistar rats. Conclusions: While A. cantonensis L3 can survive and remain infective in fish for a short period, they typically die within first few days post infection. This suggests that fish may not be significant long-term paratenic hosts for A. cantonensis but may play a temporary role in its transmission to mammals (including humans) and birds. These results are consistent with previous studies on freshwater shrimps and highlight the importance of understanding aquatic host interactions in the epidemiology of this zoonotic foodborne pathogen.

Návaznosti

GA22-26136S, projekt VaV

Název: Probíhající globální invaze zoonotické hlístice Angiostrongylus cantonensis: analýza rizik rozšíření v Evropě

Investor: Grantová agentura ČR, Probíhající globální invaze zoonotické hlístice Angiostrongylus cantonensis: analýza rizik rozšíření v Evropě

MUNI/A/1488/2021, interní kód MU

Název: Evoluční a ekologické vztahy a procesy v systémech parazit-hostitel a v biotických systémech vodních organizmů (Akronym: ParaHydro)

Investor: Masarykova univerzita, Evoluční a ekologické vztahy a procesy v systémech parazit-hostitel a v biotických systémech vodních organizmů

Citovat

ANETTOVÁ, Lucia; Jana KAČMAŘÍKOVÁ; Anna ŠIPKOVÁ; Vivienne VELIČ; Lia CAVALLO; Iva DYKOVÁ; Elena IZQUIERDO-RODRIGUEZ; Eric R MORGAN a David MODRÝ. Low potential of fish as a source of infection with Angiostrongylus cantonensis. Food Quality and Safety. Oxford University Press, 2024, roč. 8, September, s. 1-8. ISSN 2399-1399. Dostupné z: https://doi.org/10.1093/fqsafe/fyae042.

@article{2438119,
   author = {Anettová, Lucia and Kačmaříková, Jana and Šipková, Anna and Velič, Vivienne and Cavallo, Lia and Dyková, Iva and IzquierdoandRodriguez, Elena and Morgan, Eric R and Modrý, David},
   article_number = {September},
   doi = {https://doi.org/10.1093/fqsafe/fyae042},
   keywords = {Angiostrongylus cantonensis; rat lungworm; life cycle; fish; food-borne pathogen},
   language = {eng},
   issn = {2399-1399},
   journal = {Food Quality and Safety},
   title = {Low potential of fish as a source of infection with Angiostrongylus cantonensis},
   url = {https://academic.oup.com/fqs/article/doi/10.1093/fqsafe/fyae042/7750665},
   volume = {8},
   year = {2024}
}

TY  - JOUR
ID  - 2438119
AU  - Anettová, Lucia - Kačmaříková, Jana - Šipková, Anna - Velič, Vivienne - Cavallo, Lia - Dyková, Iva - Izquierdo-Rodriguez, Elena - Morgan, Eric R - Modrý, David
PY  - 2024
TI  - Low potential of fish as a source of infection with Angiostrongylus cantonensis
JF  - Food Quality and Safety
VL  - 8
IS  - September
SP  - 1-8
EP  - 1-8
PB  - Oxford University Press
SN  - 23991399
KW  - Angiostrongylus cantonensis
KW  - rat lungworm
KW  - life cycle
KW  - fish
KW  - food-borne pathogen
UR  - https://academic.oup.com/fqs/article/doi/10.1093/fqsafe/fyae042/7750665
N2  - Objectives: Fish are hypothesized to act as paratenic hosts for the zoonotic nematode Angiostrongylus cantonensis, which causes human eosinophilic meningitis.There is a lack of data confirming the relevance of fish in A. cantonensis life cycle and their contribution to human infection. Materials and Methods: We conducted a series of experiments to investigate the survival and infectivity of A. cantonensis larvae in Clarias gariepinus (catfish; n =30) and Oreochromis niloticus (tilapia; n =24). Each fish was inoculated with 10 000 third-stage larvae (L3). Larval survival was assessed through artificial digestion of fish tissues 1 week, 2 weeks, and 3 weeks post-infection. To investigate the early stages of infection, four catfish were inoculated with 10 000 L3 each and sacrificed 3 d post-infection. Quantitative polymerase chain reaction (qPCR) analysis and histopathological examination were performed to evaluate larval distribution and tissue reactions. Two infected catfish, sacrificed 1 week post-infection, were used to feed Wistar rats. Results: After 45 d, the rats did not shed first-stage larvae (L1), indicating the absence of infection. One week post-infection, dead larvae were present in the digested tissues of both fish species, and the same was observed 2 weeks and 3 weeks after exposure. qPCR analysis revealed that the intestine was the most heavily infected organ. Histopathological examination identified dead larvae within granulomas in both the testines and liver. Early-stage infection experiment showed that fish sacrificed 3 d post-inoculation contained viable L3, which was infective Wistar rats. Conclusions: While A. cantonensis L3 can survive and remain infective in fish for a short period, they typically die within first few days post infection. This suggests that fish may not be significant long-term paratenic hosts for A. cantonensis but may play a temporary role in its transmission to mammals (including humans) and birds. These results are consistent with previous studies on freshwater shrimps and highlight the importance of understanding aquatic host interactions in the epidemiology of this zoonotic foodborne pathogen.
ER  -

ANETTOVÁ, Lucia; Jana KAČMAŘÍKOVÁ; Anna ŠIPKOVÁ; Vivienne VELIČ; Lia CAVALLO; Iva DYKOVÁ; Elena IZQUIERDO-RODRIGUEZ; Eric R MORGAN a David MODRÝ. Low potential of fish as a source of infection with Angiostrongylus cantonensis. \textit{Food Quality and Safety}. Oxford University Press, 2024, roč.~8, September, s.~1-8. ISSN~2399-1399. Dostupné z: https://doi.org/10.1093/fqsafe/fyae042.

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