The archaeal community in sediments of freshwater lakes of
north-east Antarctic Peninsula: Structure and diversity

J 2024

The archaeal community in sediments of freshwater lakes of north-east Antarctic Peninsula: Structure and diversity

BURIÁNKOVÁ, Iva; Martin RULÍK; Štěpánka BÁBÍKOVÁ; Anna MOLÍKOVÁ; David NOVÁK et al.

Základní údaje

Originální název

The archaeal community in sediments of freshwater lakes of north-east Antarctic Peninsula: Structure and diversity

Autoři

BURIÁNKOVÁ, Iva; Martin RULÍK; Štěpánka BÁBÍKOVÁ; Anna MOLÍKOVÁ; David NOVÁK ; Jan LOCHMAN a Monika VÍTĚZOVÁ

Vydání

Czech Polar Reports, Masaryk University Press, 2024, 1805-0689

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10606 Microbiology

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 0.800

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/24:00137228

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

Archaea; methanogens; Antarctica; James Ross Island; NGS; lake sediments; biodiversity

Štítky

14313050, 14314010, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 10. 12. 2024 12:16, Mgr. Marie Novosadová Šípková, DiS.

Anotace

V originále

This research represents the first attempt to study the structure and diversity of the archaeal and methanogenic archaeal community in selected lakes around the Czech polar station J.G. Mendel on James Ross Island (JRI), Antarctica. Sediment samples from a total of 19 of JRI and the nearby Vega Island and Long Island, were analyzed using 16S rRNA and mcrA genes sequencing and real-time qPCR. Contrary to the sequences retrieved by 16S rRNA analysis, many more reads belonging to methanogens were found with mcrA gene sequencing. Generally, archaea represented only a small proportion (0–8.8%) of the total prokaryotic community. With the exception of lakes in the Solorina Valley and Lagoons Mesa area and Lake Esmeralda on Vega Island, methanogenic archaea made up a small proportion of the archaea present in most lakes. The genera Methanothrix and Methanosarcina were identified as the predominant methanogenic representatives in the lake samples. Sequences of representatives belonging to Methanothermobacter sp. and Methanomassiliicoccales and a high proportion of sequences belonging to Methanoperedens-like archaea, methanotrophs that associate anaerobic methane oxidation with denitrification, were recorded for the first time in Antarctica. It is also the first time that the presence of the genus Methanobacterium has been detected to such a large extent. Generally, sequences of the methanogens which might be involved in all three pathways of methane production were found in our samples, indicating the broad metabolic potential of the methanogens present. Individual lakes from one area shared much higher similarity in their methanogenic diversity with the lakes from another area rather than with the lakes within the same area, suggesting that a lake location is probably not the main factor influencing the diversity of the methanogens. Indeed, archaeal and methanogenic community structure and mcrA gene copy numbers varied even within a single lake, suggesting that more sampling within a single lake, preferably at different times of the year, will be necessary in the future for more comprehensive information. Although this is an initial study, our research unambiguously provides evidence that the lakes of the JRI and surrounding islands may be potential sources of new archaeal species or metabolic pathways.

Citovat

BURIÁNKOVÁ, Iva; Martin RULÍK; Štěpánka BÁBÍKOVÁ; Anna MOLÍKOVÁ; David NOVÁK; Jan LOCHMAN a Monika VÍTĚZOVÁ. The archaeal community in sediments of freshwater lakes of north-east Antarctic Peninsula: Structure and diversity. Czech Polar Reports. Masaryk University Press, 2024, roč. 14, č. 1, s. 10-37. ISSN 1805-0689. Dostupné z: https://doi.org/10.5817/CPR2024-1-2.

@article{2438418,
   author = {Buriánková, Iva and Rulík, Martin and Bábíková, Štěpánka and Molíková, Anna and Novák, David and Lochman, Jan and Vítězová, Monika},
   article_number = {1},
   doi = {https://doi.org/10.5817/CPR2024-1-2},
   keywords = {Archaea; methanogens; Antarctica; James Ross Island; NGS; lake sediments; biodiversity},
   language = {eng},
   issn = {1805-0689},
   journal = {Czech Polar Reports},
   title = {The archaeal community in sediments of freshwater lakes of north-east Antarctic Peninsula: Structure and diversity},
   url = {https://journals.muni.cz/CPR/article/view/39281},
   volume = {14},
   year = {2024}
}

TY  - JOUR
ID  - 2438418
AU  - Buriánková, Iva - Rulík, Martin - Bábíková, Štěpánka - Molíková, Anna - Novák, David - Lochman, Jan - Vítězová, Monika
PY  - 2024
TI  - The archaeal community in sediments of freshwater lakes of north-east Antarctic Peninsula: Structure and diversity
JF  - Czech Polar Reports
VL  - 14
IS  - 1
SP  - 10-37
EP  - 10-37
PB  - Masaryk University Press
SN  - 18050689
KW  - Archaea
KW  - methanogens
KW  - Antarctica
KW  - James Ross Island
KW  - NGS
KW  - lake sediments
KW  - biodiversity
UR  - https://journals.muni.cz/CPR/article/view/39281
N2  - This research represents the first attempt to study the structure and diversity of the archaeal and methanogenic archaeal community in selected lakes around the Czech polar station J.G. Mendel on James Ross Island (JRI), Antarctica. Sediment samples from a total of 19 of JRI and the nearby Vega Island and Long Island, were analyzed using 16S rRNA and mcrA genes sequencing and real-time qPCR. Contrary to the sequences retrieved by 16S rRNA analysis, many more reads belonging to methanogens were found with mcrA gene sequencing. Generally, archaea represented only a small proportion (0–8.8%) of the total prokaryotic community. With the exception of lakes in the Solorina Valley and Lagoons Mesa area and Lake Esmeralda on Vega Island, methanogenic archaea made up a small proportion of the archaea present in most lakes. The genera Methanothrix and Methanosarcina were identified as the predominant methanogenic representatives in the lake samples. Sequences of representatives belonging to Methanothermobacter sp. and Methanomassiliicoccales and a high proportion of sequences belonging to Methanoperedens-like archaea, methanotrophs that associate anaerobic methane oxidation with denitrification, were recorded for the first time in Antarctica. It is also the first time that the presence of the genus Methanobacterium has been detected to such a large extent. Generally, sequences of the methanogens which might be involved in all three pathways of methane production were found in our samples, indicating the broad metabolic potential of the methanogens present. Individual lakes from one area shared much higher similarity in their methanogenic diversity with the lakes from another area rather than with the lakes within the same area, suggesting that a lake location is probably not the main factor influencing the diversity of the methanogens. Indeed, archaeal and methanogenic community structure and mcrA gene copy numbers varied even within a single lake, suggesting that more sampling within a single lake, preferably at different times of the year, will be necessary in the future for more comprehensive information. Although this is an initial study, our research unambiguously provides evidence that the lakes of the JRI and surrounding islands may be potential sources of new archaeal species or metabolic pathways.
ER  -

BURIÁNKOVÁ, Iva; Martin RULÍK; Štěpánka BÁBÍKOVÁ; Anna MOLÍKOVÁ; David NOVÁK; Jan LOCHMAN a Monika VÍTĚZOVÁ. The archaeal community in sediments of freshwater lakes of north-east Antarctic Peninsula: Structure and diversity. \textit{Czech Polar Reports}. Masaryk University Press, 2024, roč.~14, č.~1, s.~10-37. ISSN~1805-0689. Dostupné z: https://doi.org/10.5817/CPR2024-1-2.

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