CRISPR-Cas10 Engineering of Staphylococcus aureus
bacteriophages for biosensing applications

a 2024

CRISPR-Cas10 Engineering of Staphylococcus aureus bacteriophages for biosensing applications

ŠIMEČKOVÁ, Hana; Pavel PAYNE; Zdeněk FARKA; Roman PANTŮČEK; Pavel PLEVKA et. al.

Základní údaje

Originální název

CRISPR-Cas10 Engineering of Staphylococcus aureus bacteriophages for biosensing applications

Autoři

ŠIMEČKOVÁ, Hana; Pavel PAYNE; Zdeněk FARKA; Roman PANTŮČEK; Pavel PLEVKA a Ivana MAŠLAŇOVÁ

Vydání

Meeting of the National Institute of Virology and Bacteriology (NIVB) in Kutná Hora 2024, 2024

Další údaje

Typ výsledku

Konferenční abstrakt

Utajení

není předmětem státního či obchodního tajemství

Odkazy

NIVB Meeting 2024 Abstract Book

ISSN

Klíčová slova anglicky

Staphylococcus aureus; bacteriophages; CRISPR-Cas; biosensors; phage therapy

Změněno: 10. 10. 2024 09:12, Mgr. Hana Šimečková, Ph.D.

Anotace

V originále

The increasing prevalence of antimicrobial-resistant bacterial pathogens, including multidrug-resistant strains of Staphylococcus aureus, has intensified the need for alternative therapeutic approaches. Particular attention is being paid to phage therapy, which utilizes viral particles infecting bacteria as a key component in the treatment process. Significant advantages of bacteriophages as lytic agents include high specificity (which reduces the impact on natural microflora), their ability to penetrate biofilms, and the potential for phage property modification. The CRISPR-Cas10 technology, a powerful tool for precise genetic engineering, enables targeted modifications of viral genomes by facilitating the selection of recombinant bacteriophages.1 This method holds potential for constructing viral particles with enhanced therapeutic properties, such as an expanded host range or improved lytic efficiency. The novel, unique characteristics also broaden the scope for their application in biotechnology. The use of modified bacteriophages in combination with biosensing technologies opens up new possibilities for applications in diagnostics and environmental monitoring, as well as in research and development. In our study, we focus on modifying the structural proteins of S. aureus therapeutic phages by adding a polyhistidine (His) tag to facilitate the oriented binding of particles to a biosensor chip. To monitor the binding kinetics and confirm particle attachment, we will use Bio-Layer Interferometry (BLI) and Surface Plasmon Resonance (SPR) biosensors.

Návaznosti

LX22NPO5103, projekt VaV

Název: Národní institut virologie a bakteriologie (Akronym: NIVB)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Národní institut virologie a bakteriologie, 5.1 EXCELES

Citovat

ŠIMEČKOVÁ, Hana; Pavel PAYNE; Zdeněk FARKA; Roman PANTŮČEK; Pavel PLEVKA a Ivana MAŠLAŇOVÁ. CRISPR-Cas10 Engineering of Staphylococcus aureus bacteriophages for biosensing applications. In Meeting of the National Institute of Virology and Bacteriology (NIVB) in Kutná Hora 2024. 2024. ISSN 2336-7210.

@proceedings{2441917,
   author = {Šimečková, Hana and Payne, Pavel and Farka, Zdeněk and Pantůček, Roman and Plevka, Pavel and Mašlaňová, Ivana},
   booktitle = {Meeting of the National Institute of Virology and Bacteriology (NIVB) in Kutná Hora 2024},
   keywords = {Staphylococcus aureus; bacteriophages; CRISPR-Cas; biosensors; phage therapy},
   title = {CRISPR-Cas10 Engineering of Staphylococcus aureus bacteriophages for biosensing applications},
   url = {http://www.ccsss.cz/index.php/ccsss/issue/view/48},
   year = {2024}
}

TY  - CONF
ID  - 2441917
AU  - Šimečková, Hana - Payne, Pavel - Farka, Zdeněk - Pantůček, Roman - Plevka, Pavel - Mašlaňová, Ivana
PY  - 2024
TI  - CRISPR-Cas10 Engineering of Staphylococcus aureus bacteriophages for biosensing applications
KW  - Staphylococcus aureus
KW  - bacteriophages
KW  - CRISPR-Cas
KW  - biosensors
KW  - phage therapy
UR  - http://www.ccsss.cz/index.php/ccsss/issue/view/48
N2  - The increasing prevalence of antimicrobial-resistant bacterial pathogens, including multidrug-resistant strains of Staphylococcus aureus, has intensified the need for alternative therapeutic approaches. Particular attention is being paid to phage therapy, which utilizes viral particles infecting bacteria as a key component in the treatment process. Significant advantages of bacteriophages as lytic agents include high specificity (which reduces the impact on natural microflora), their ability to penetrate biofilms, and the potential for phage property modification. The CRISPR-Cas10 technology, a powerful tool for precise genetic engineering, enables targeted modifications of viral genomes by facilitating the selection of recombinant bacteriophages.1 This method holds potential for constructing viral particles with enhanced therapeutic properties, such as an expanded host range or improved lytic efficiency. The novel, unique characteristics also broaden the scope for their application in biotechnology. The use of modified bacteriophages in combination with biosensing technologies opens up new possibilities for applications in diagnostics and environmental monitoring, as well as in research and development. In our study, we focus on modifying the structural proteins of S. aureus therapeutic phages by adding a polyhistidine (His) tag to facilitate the oriented binding of particles to a biosensor chip. To monitor the binding kinetics and confirm particle attachment, we will use Bio-Layer Interferometry (BLI) and Surface Plasmon Resonance (SPR) biosensors.
ER  -

ŠIMEČKOVÁ, Hana; Pavel PAYNE; Zdeněk FARKA; Roman PANTŮČEK; Pavel PLEVKA a Ivana MAŠLAŇOVÁ. CRISPR-Cas10 Engineering of Staphylococcus aureus bacteriophages for biosensing applications. In \textit{Meeting of the National Institute of Virology and Bacteriology (NIVB) in Kutná Hora 2024}. 2024. ISSN~2336-7210.

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