Spread of staphylococcal mobilome due to different therapeutic
approaches

a 2024

Spread of staphylococcal mobilome due to different therapeutic approaches

BOTKA, Tibor; Kristína ROVŇÁKOVÁ; Adam VINCO; Lenka KOSEČKOVÁ MICENKOVÁ; Roman PANTŮČEK et al.

Základní údaje

Originální název

Spread of staphylococcal mobilome due to different therapeutic approaches

Autoři

BOTKA, Tibor; Kristína ROVŇÁKOVÁ; Adam VINCO; Lenka KOSEČKOVÁ MICENKOVÁ a Roman PANTŮČEK

Vydání

19th International Symposium on Staphylococci and Staphylococcal Infections, 2024

Další údaje

Jazyk

angličtina

Typ výsledku

Konferenční abstrakt

Obor

10608 Biochemistry and molecular biology

Stát vydavatele

Austrálie

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/24:00139843

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

Staphylococcus; bacteriophage; PICI; transduction; phage antibiotics synergy; phage therapy

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 28. 12. 2024 14:59, prof. RNDr. Roman Pantůček, Ph.D.

Anotace

V originále

Aim: Characterize staphylococcal mobilome induced by various agents, including antibiotic and phage therapy, and their combination. Background: Mobile genetic elements, like phages and chromosomal islands, represent a major force in the emergence of new pathogenic and drug-resistant Staphylococcus spp. strains. On the other hand, lytic phages can be used in the therapy of bacterial infections as an alternative or complement to antibiotics. With increasing knowledge of horizontal transfer, it is evident that some antibiotics, such as fluoroquinolones, can mobilize genetic elements, thereby spreading antibiotic resistance and virulence factors. Methods: A phage-inducible chromosomal island (PICI) and prophage E72 were transferred into the Staphylococcus epidermidis strain 1457 via transduction and lysogenization. The obtained strain 1457LA was treated with ciprofloxacin, lytic phage 812h1, and their combination. The optical density of the treated culture and titer of the induced and lytic phages were determined. DNA of strain 1457LA and from the released virions was extracted and sequenced. The amount of genetic elements released after each treatment was determined and compared. Results: 1457LA treatment with ciprofloxacin minimum inhibitory concentration did not lead to culture eradication, while almost 40% of the genomes detected in virions belonged to induced phage E72 and 60% to the PICI. After infection with phage 812h1, more than 99% of the genomes belonged to this lytic phage. In the case of the combined treatment, the genome of the lytic phage was present in 60 to 99.9% of the virions, depending on the input ratio of the phage. The genomes of PICI and E72 were approximately equally represented in the rest of the virions. The combined treatment resulted in the most significant decrease in culture density. Conclusions: Combination treatment resulted in a significant reduction in the amount of genetic elements released compared to antibiotic treatment and the highest decrease in culture optical density.

Návaznosti

FW10010112, projekt VaV

Název: Kombinovaná fágová terapie pro podporu léčby chronických respiračních onemocnění.

Investor: Technologická agentura ČR, Kombinovaná fágová terapie pro podporu léčby chronických respiračních onemocnění

NU21J-05-00035, projekt VaV

Název: Synergie lytických bakteriofágů a antibiotik v léčbě povrchových infekcí způsobených Staphylococcus aureus (Akronym: PAS-based therapy of S. aureus infections)

Investor: Ministerstvo zdravotnictví ČR, Synergy of lytic bacteriophages and antibiotics in the therapy of topical infections of Staphylococcus aureus, Podprogram 2 - juniorský - výzkumníci do 35 let

Citovat

BOTKA, Tibor; Kristína ROVŇÁKOVÁ; Adam VINCO; Lenka KOSEČKOVÁ MICENKOVÁ a Roman PANTŮČEK. Spread of staphylococcal mobilome due to different therapeutic approaches. In 19th International Symposium on Staphylococci and Staphylococcal Infections. 2024.

@proceedings{2441946,
   author = {Botka, Tibor and Rovňáková, Kristína and Vinco, Adam and Kosečková Micenková, Lenka and Pantůček, Roman},
   booktitle = {19th International Symposium on Staphylococci and Staphylococcal Infections},
   keywords = {Staphylococcus; bacteriophage; PICI; transduction; phage antibiotics synergy; phage therapy},
   language = {eng},
   title = {Spread of staphylococcal mobilome due to different therapeutic approaches},
   url = {https://isssi2024.org/},
   year = {2024}
}

TY  - CONF
ID  - 2441946
AU  - Botka, Tibor - Rovňáková, Kristína - Vinco, Adam - Kosečková Micenková, Lenka - Pantůček, Roman
PY  - 2024
TI  - Spread of staphylococcal mobilome due to different therapeutic approaches
KW  - Staphylococcus
KW  - bacteriophage
KW  - PICI
KW  - transduction
KW  - phage antibiotics synergy
KW  - phage therapy
UR  - https://isssi2024.org/
N2  - Aim: Characterize staphylococcal mobilome induced by various agents, including antibiotic and phage therapy, and their combination. Background: Mobile genetic elements, like phages and chromosomal islands, represent a major force in the emergence of new pathogenic and drug-resistant Staphylococcus spp. strains. On the other hand, lytic phages can be used in the therapy of bacterial infections as an alternative or complement to antibiotics. With increasing knowledge of horizontal transfer, it is evident that some antibiotics, such as fluoroquinolones, can mobilize genetic elements, thereby spreading antibiotic resistance and virulence factors. Methods: A phage-inducible chromosomal island (PICI) and prophage E72 were transferred into the Staphylococcus epidermidis strain 1457 via transduction and lysogenization. The obtained strain 1457LA was treated with ciprofloxacin, lytic phage 812h1, and their combination. The optical density of the treated culture and titer of the induced and lytic phages were determined. DNA of strain 1457LA and from the released virions was extracted and sequenced. The amount of genetic elements released after each treatment was determined and compared. Results: 1457LA treatment with ciprofloxacin minimum inhibitory concentration did not lead to culture eradication, while almost 40% of the genomes detected in virions belonged to induced phage E72 and 60% to the PICI. After infection with phage 812h1, more than 99% of the genomes belonged to this lytic phage. In the case of the combined treatment, the genome of the lytic phage was present in 60 to 99.9% of the virions, depending on the input ratio of the phage. The genomes of PICI and E72 were approximately equally represented in the rest of the virions. The combined treatment resulted in the most significant decrease in culture density. Conclusions: Combination treatment resulted in a significant reduction in the amount of genetic elements released compared to antibiotic treatment and the highest decrease in culture optical density.
ER  -

BOTKA, Tibor; Kristína ROVŇÁKOVÁ; Adam VINCO; Lenka KOSEČKOVÁ MICENKOVÁ a Roman PANTŮČEK. Spread of staphylococcal mobilome due to different therapeutic approaches. In \textit{19th International Symposium on Staphylococci and Staphylococcal Infections}. 2024.

Přehled o publikaci