Prevalence of Borrelia and Rickettsia in Ixodes ricinus from
Chosen Urban and Protected Areas in Poland and the Czech
Republic

J 2024

Prevalence of Borrelia and Rickettsia in Ixodes ricinus from Chosen Urban and Protected Areas in Poland and the Czech Republic

KIEWRA, Dorota; Dagmara DYCZKO; Alena ŽÁKOVSKÁ a Helena NEJEZCHLEBOVÁ

Základní údaje

Originální název

Prevalence of Borrelia and Rickettsia in Ixodes ricinus from Chosen Urban and Protected Areas in Poland and the Czech Republic

Autoři

KIEWRA, Dorota; Dagmara DYCZKO; Alena ŽÁKOVSKÁ a Helena NEJEZCHLEBOVÁ

Vydání

Insects, MDPI, 2024, 2075-4450

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10606 Microbiology

Stát vydavatele

Švýcarsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 2.900

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14410/24:00137328

Organizační jednotka

Pedagogická fakulta

Klíčová slova anglicky

Borrelia spp.; Ixodes ricinus; protected areas; Rickettsia spp.; tick-borne risk; urban areas

Štítky

14314010, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 12. 12. 2024 14:09, Mgr. Marie Novosadová Šípková, DiS.

Anotace

V originále

(1) Background: Ixodes ricinus is responsible for the spreading of medically important pathogens. Monitoring the level of tick infection in various areas is essential for determining the potential tick-born risk. This study aimed to detect Borrelia spp. and Rickettsia spp. in I. ricinus ticks collected in urban and protected areas both in Poland and the Czech Republic. (2) Methods: Ticks were collected by flagging in the years 2016–2017. Borrelia spp. was detected using nested PCR targeting the flaB gene and Rickettsia spp. using nested PCR targeting gltA. (3) Results: In total, DNA of Borrelia spp. was detected in 25.9% of samples. Ticks collected in Poland were more infected compared to the Czech Republic and ticks collected in protected areas were more infected with Borrelia spp. than ticks collected in urban areas. The RFLP analysis showed the occurrence of B. afzelii and B. garinii in both countries, and additionally B. valaisiana, B. burgdorferi s.s., and B. miyamotoi in Poland. Rickettsia spp. was detected in 17.4% of I. ricinus, with comparable infection level in both countries; however, regional differences were observed. (4) Conclusion: The regional differences in Borrelia spp. and Rickettsia spp. prevalence in I. ricinus indicate the complexity of factors influencing the level of infection and underline the need for adaptation public health surveillance strategies in each region.

Citovat

KIEWRA, Dorota; Dagmara DYCZKO; Alena ŽÁKOVSKÁ a Helena NEJEZCHLEBOVÁ. Prevalence of Borrelia and Rickettsia in Ixodes ricinus from Chosen Urban and Protected Areas in Poland and the Czech Republic. Insects. MDPI, 2024, roč. 15, č. 10, s. 1-10. ISSN 2075-4450. Dostupné z: https://doi.org/10.3390/insects15100785.

@article{2442877,
   author = {Kiewra, Dorota and Dyczko, Dagmara and Žákovská, Alena and Nejezchlebová, Helena},
   article_number = {10},
   doi = {https://doi.org/10.3390/insects15100785},
   keywords = {Borrelia spp.; Ixodes ricinus; protected areas; Rickettsia spp.; tick-borne risk; urban areas},
   language = {eng},
   issn = {2075-4450},
   journal = {Insects},
   title = {Prevalence of Borrelia and Rickettsia in Ixodes ricinus from Chosen Urban and Protected Areas in Poland and the Czech Republic},
   url = {https://www.mdpi.com/2075-4450/15/10/785},
   volume = {15},
   year = {2024}
}

TY  - JOUR
ID  - 2442877
AU  - Kiewra, Dorota - Dyczko, Dagmara - Žákovská, Alena - Nejezchlebová, Helena
PY  - 2024
TI  - Prevalence of Borrelia and Rickettsia in Ixodes ricinus from Chosen Urban and Protected Areas in Poland and the Czech Republic
JF  - Insects
VL  - 15
IS  - 10
SP  - 1-10
EP  - 1-10
PB  - MDPI
SN  - 20754450
KW  - Borrelia spp.
KW  - Ixodes ricinus
KW  - protected areas
KW  - Rickettsia spp.
KW  - tick-borne risk
KW  - urban areas
UR  - https://www.mdpi.com/2075-4450/15/10/785
N2  - (1) Background: Ixodes ricinus is responsible for the spreading of medically important pathogens. Monitoring the level of tick infection in various areas is essential for determining the potential tick-born risk. This study aimed to detect Borrelia spp. and Rickettsia spp. in I. ricinus ticks collected in urban and protected areas both in Poland and the Czech Republic. (2) Methods: Ticks were collected by flagging in the years 2016–2017. Borrelia spp. was detected using nested PCR targeting the flaB gene and Rickettsia spp. using nested PCR targeting gltA. (3) Results: In total, DNA of Borrelia spp. was detected in 25.9% of samples. Ticks collected in Poland were more infected compared to the Czech Republic and ticks collected in protected areas were more infected with Borrelia spp. than ticks collected in urban areas. The RFLP analysis showed the occurrence of B. afzelii and B. garinii in both countries, and additionally B. valaisiana, B. burgdorferi s.s., and B. miyamotoi in Poland. Rickettsia spp. was detected in 17.4% of I. ricinus, with comparable infection level in both countries; however, regional differences were observed. (4) Conclusion: The regional differences in Borrelia spp. and Rickettsia spp. prevalence in I. ricinus indicate the complexity of factors influencing the level of infection and underline the need for adaptation public health surveillance strategies in each region.
ER  -

KIEWRA, Dorota; Dagmara DYCZKO; Alena ŽÁKOVSKÁ a Helena NEJEZCHLEBOVÁ. Prevalence of Borrelia and Rickettsia in Ixodes ricinus from Chosen Urban and Protected Areas in Poland and the Czech Republic. \textit{Insects}. MDPI, 2024, roč.~15, č.~10, s.~1-10. ISSN~2075-4450. Dostupné z: https://doi.org/10.3390/insects15100785.

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