Joint forces of mass spectrometric techniques (ICP-MS and
MALDI-TOF-MS) and fluorescence spectrometry in the study of
platinum-based cytostatic drugs interactions with
metallothionein MT2 and MT3

J 2024

Joint forces of mass spectrometric techniques (ICP-MS and MALDI-TOF-MS) and fluorescence spectrometry in the study of platinum-based cytostatic drugs interactions with metallothionein MT2 and MT3

PAVELICOVA, Kristyna; Tomas DO; Markéta VEJVODOVÁ; Tomáš VACULOVIČ; Kinga NOWAK et al.

Základní údaje

Originální název

Joint forces of mass spectrometric techniques (ICP-MS and MALDI-TOF-MS) and fluorescence spectrometry in the study of platinum-based cytostatic drugs interactions with metallothionein MT2 and MT3

Autoři

PAVELICOVA, Kristyna; Tomas DO; Markéta VEJVODOVÁ; Tomáš VACULOVIČ; Kinga NOWAK; Magdalena MATCZUK; Sylwia WU; Artur KREZEL; Vojtech ADAM a Marketa VACULOVICOVA

Vydání

Talanta, AMSTERDAM, Elsevier, 2024, 0039-9140

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10406 Analytical chemistry

Stát vydavatele

Nizozemské království

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 6.100

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/24:00137330

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

Metal ions; Dimers; Laser ablation; Inductively coupled plasma; Mass spectrometry

Štítky

14313010, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 5. 11. 2024 15:53, Mgr. Pavla Foltynová, Ph.D.

Anotace

V originále

Herby, the interaction of metallothioneins with commonly used Pt-based anticancer drugs - cisplatin, carboplatin, and oxaliplatin - was investigated using the combined power of elemental (i.e. LA-ICP-MS, CE-ICP-MS) and molecular (i.e. MALDI-TOF-MS) analytical techniques providing not only required information about the interaction, but also the benefit of low sample consumption. The amount of Cd and Pt incorporated within the protein was determined for protein monomers and dimer/oligomers formed by non-oxidative dimerization. Moreover, fluorescence spectrometry using Zn2+-selective fluorescent indicator - FluoZin3 - was employed to monitor the ability of Pt drugs to release natively occurring Zn from the protein molecule. The investigation was carried out using two protein isoforms (i.e. MT2, MT3), and significant differences in behaviour of these two isoforms were observed. The main attention was paid to elucidating whether the protein dimerization/oligomerization may be the reason for the potential failure of the anticancer therapy based on these drugs. Based on the results, it was demonstrated that the interaction of MT2 (both monomers and dimers) interacted with Pt drugs significantly less compared to MT3 (both monomers and dimers). Also, a significant difference between monomeric and dimeric forms (both MT2 and MT3) was not observed. This may suggest that dimer formation is not the key factor leading to the inactivation of Pt drugs.

Návaznosti

MUNI/A/1575/2023, interní kód MU

Název: Metody chemické a fyzikálně-chemické analýzy pro studium přírodních a syntetických materiálů.

Investor: Masarykova univerzita, Metody chemické a fyzikálně-chemické analýzy pro studium přírodních a syntetických materiálů.

Citovat

PAVELICOVA, Kristyna; Tomas DO; Markéta VEJVODOVÁ; Tomáš VACULOVIČ; Kinga NOWAK; Magdalena MATCZUK; Sylwia WU; Artur KREZEL; Vojtech ADAM a Marketa VACULOVICOVA. Joint forces of mass spectrometric techniques (ICP-MS and MALDI-TOF-MS) and fluorescence spectrometry in the study of platinum-based cytostatic drugs interactions with metallothionein MT2 and MT3. Talanta. AMSTERDAM: Elsevier, 2024, roč. 274, JUL, s. 125920-125927. ISSN 0039-9140. Dostupné z: https://doi.org/10.1016/j.talanta.2024.125920.

@article{2442939,
   author = {Pavelicova, Kristyna and Do, Tomas and Vejvodová, Markéta and Vaculovič, Tomáš and Nowak, Kinga and Matczuk, Magdalena and Wu, Sylwia and Krezel, Artur and Adam, Vojtech and Vaculovicova, Marketa},
   article_location = {AMSTERDAM},
   article_number = {JUL},
   doi = {https://doi.org/10.1016/j.talanta.2024.125920},
   keywords = {Metal ions; Dimers; Laser ablation; Inductively coupled plasma; Mass spectrometry},
   language = {eng},
   issn = {0039-9140},
   journal = {Talanta},
   title = {Joint forces of mass spectrometric techniques (ICP-MS and MALDI-TOF-MS) and fluorescence spectrometry in the study of platinum-based cytostatic drugs interactions with metallothionein MT2 and MT3},
   url = {https://www.sciencedirect.com/science/article/pii/S0039914024002996?via%3Dihub},
   volume = {274},
   year = {2024}
}

TY  - JOUR
ID  - 2442939
AU  - Pavelicova, Kristyna - Do, Tomas - Vejvodová, Markéta - Vaculovič, Tomáš - Nowak, Kinga - Matczuk, Magdalena - Wu, Sylwia - Krezel, Artur - Adam, Vojtech - Vaculovicova, Marketa
PY  - 2024
TI  - Joint forces of mass spectrometric techniques (ICP-MS and MALDI-TOF-MS) and fluorescence spectrometry in the study of platinum-based cytostatic drugs interactions with metallothionein MT2 and MT3
JF  - Talanta
VL  - 274
IS  - JUL
SP  - 125920
EP  - 125920
PB  - Elsevier
SN  - 00399140
KW  - Metal ions
KW  - Dimers
KW  - Laser ablation
KW  - Inductively coupled plasma
KW  - Mass spectrometry
UR  - https://www.sciencedirect.com/science/article/pii/S0039914024002996?via%3Dihub
N2  - Herby, the interaction of metallothioneins with commonly used Pt-based anticancer drugs - cisplatin, carboplatin, and oxaliplatin - was investigated using the combined power of elemental (i.e. LA-ICP-MS, CE-ICP-MS) and molecular (i.e. MALDI-TOF-MS) analytical techniques providing not only required information about the interaction, but also the benefit of low sample consumption. The amount of Cd and Pt incorporated within the protein was determined for protein monomers and dimer/oligomers formed by non-oxidative dimerization. Moreover, fluorescence spectrometry using Zn2+-selective fluorescent indicator - FluoZin3 - was employed to monitor the ability of Pt drugs to release natively occurring Zn from the protein molecule. The investigation was carried out using two protein isoforms (i.e. MT2, MT3), and significant differences in behaviour of these two isoforms were observed. The main attention was paid to elucidating whether the protein dimerization/oligomerization may be the reason for the potential failure of the anticancer therapy based on these drugs. Based on the results, it was demonstrated that the interaction of MT2 (both monomers and dimers) interacted with Pt drugs significantly less compared to MT3 (both monomers and dimers). Also, a significant difference between monomeric and dimeric forms (both MT2 and MT3) was not observed. This may suggest that dimer formation is not the key factor leading to the inactivation of Pt drugs.
ER  -

PAVELICOVA, Kristyna; Tomas DO; Markéta VEJVODOVÁ; Tomáš VACULOVIČ; Kinga NOWAK; Magdalena MATCZUK; Sylwia WU; Artur KREZEL; Vojtech ADAM a Marketa VACULOVICOVA. Joint forces of mass spectrometric techniques (ICP-MS and MALDI-TOF-MS) and fluorescence spectrometry in the study of platinum-based cytostatic drugs interactions with metallothionein MT2 and MT3. \textit{Talanta}. AMSTERDAM: Elsevier, 2024, roč.~274, JUL, s.~125920-125927. ISSN~0039-9140. Dostupné z: https://doi.org/10.1016/j.talanta.2024.125920.

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