Utilizing direct molecular methods for screening of invasive
helminth species   in wild ruminants in the Czech Republic

a 2024

Utilizing direct molecular methods for screening of invasive helminth species in wild ruminants in the Czech Republic

ŠKORPÍKOVÁ, Lucie; Jana ILGOVÁ; Jaroslav VADLEJCH; Jan MAGDÁLEK; Radim PLHAL et al.

Základní údaje

Originální název

Utilizing direct molecular methods for screening of invasive helminth species in wild ruminants in the Czech Republic

Název česky

Využití přímých molekulárních metod pro screening invazních druhů helmintů u volně žijících přežvýkavců v České republice

Autoři

ŠKORPÍKOVÁ, Lucie; Jana ILGOVÁ; Jaroslav VADLEJCH; Jan MAGDÁLEK; Radim PLHAL; Jakub DRIMAJ; Ondřej MIKULKA; Martin KAŠNÝ a Nikol RESLOVÁ

Vydání

28TH HELMINTHOLOGICAL DAYS, 2024

Další údaje

Jazyk

angličtina

Typ výsledku

Konferenční abstrakt

Obor

10613 Zoology

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Označené pro přenos do RIV

Organizační jednotka

Přírodovědecká fakulta

ISBN

978-80-11-05478-6

Klíčová slova česky

Invazní druhy, Fascioloides magna, Ashworthius sidemi, divočí přežvýkavci, multiplex real-time PCR

Klíčová slova anglicky

Invasive species, Fascioloides magna, Ashworthius sidemi, wild ruminants, multiplex real-time PCR

Změněno: 23. 2. 2026 09:00, Mgr. Lucie Škorpíková, Ph.D.

Anotace

V originále

In recent years, biological communities have undergone significant changes due to various factors such as globalization, climate change, and the increasing socio-economic activities of humans. These factors have led to the introduction of several non-native organisms to new habitats, which is also associated with the risk of co-introducing new parasitic species known as "biological hitchhikers". Those parasites that successfully adapt to new environments and sympatric host species and successfully reproduce and spread are classified as invasive. The primary objective of our research was to achieve a comprehensive understanding of the current prevalence of two invasive parasite species, specifically the haematophagous abomasal nematode Ashworthius sidemi and the giant liver fluke Fascioloides magna, and to uncover their impact on wild ruminant populations. For these purposes, faecal samples of wild ruminants were collected from various locations across the Czech Republic between 2022 and 2024. These included 306 samples of roe deer (Capreolus capreolus) from 27 localities, 347 samples of red deer (Cervus elaphus) from 28 localities, 90 samples of fallow deer (Dama dama) from 10 localities, and 54 samples of mouflon (Ovis musimon) from 5 localities. To identify the selected parasite species at the molecular level, sensitive diagnostic methods based on real-time PCR with TaqMan probes were implemented for specific detection of their DNA directly in animal faeces. According to the findings, A. sidemi and F. magna are still present and spreading among ruminants in game reserves and hunting grounds in the Czech Republic. The red deer has been found to be a major carrier of an invasive nematode A. sidemi, which has been found in 89% of the areas where these animals were studied. Moreover, the giant liver fluke F. magna continues to pose a possible threat in the Central and South Bohemian Regions, particularly to domestic ruminants whose pastures may be contaminated by free-ranging game.

Návaznosti

SS05010070, projekt VaV

Název: Šíření invazních druhů parazitů a jejich drtivé dopady na biologickou rozmanitost původních druhů přežvýkavců

Investor: Technologická agentura ČR, Šíření invazních druhů parazitů a jejich drtivé dopady na biologickou rozmanitost původních druhů přežvýkavců

Citovat

ŠKORPÍKOVÁ, Lucie; Jana ILGOVÁ; Jaroslav VADLEJCH; Jan MAGDÁLEK; Radim PLHAL; Jakub DRIMAJ; Ondřej MIKULKA; Martin KAŠNÝ a Nikol RESLOVÁ. Utilizing direct molecular methods for screening of invasive helminth species in wild ruminants in the Czech Republic. In 28TH HELMINTHOLOGICAL DAYS. 2024. ISBN 978-80-11-05478-6.

@proceedings{2443578,
   author = {Škorpíková, Lucie and Ilgová, Jana and Vadlejch, Jaroslav and Magdálek, Jan and Plhal, Radim and Drimaj, Jakub and Mikulka, Ondřej and Kašný, Martin and Reslová, Nikol},
   booktitle = {28TH HELMINTHOLOGICAL DAYS},
   keywords = {Invasive species, Fascioloides magna, Ashworthius sidemi, wild ruminants, multiplex real-time PCR},
   language = {eng},
   isbn = {978-80-11-05478-6},
   title = {Utilizing direct molecular methods for screening of invasive helminth species in wild ruminants in the Czech Republic},
   url = {https://hd2024.natur.cuni.cz/},
   year = {2024}
}

TY  - CONF
ID  - 2443578
AU  - Škorpíková, Lucie - Ilgová, Jana - Vadlejch, Jaroslav - Magdálek, Jan - Plhal, Radim - Drimaj, Jakub - Mikulka, Ondřej - Kašný, Martin - Reslová, Nikol
PY  - 2024
TI  - Utilizing direct molecular methods for screening of invasive helminth species in wild ruminants in the Czech Republic
SN  - 9788011054786
KW  - Invasive species, Fascioloides magna, Ashworthius sidemi, wild ruminants, multiplex real-time PCR
UR  - https://hd2024.natur.cuni.cz/
N2  - In recent years, biological communities have undergone significant changes due to various factors such as globalization, climate change, and the increasing socio-economic activities of humans. These factors have led to the introduction of several non-native organisms to new habitats, which is also associated with the risk of co-introducing new parasitic species known as "biological hitchhikers". Those parasites that successfully adapt to new environments and sympatric host species and successfully reproduce and spread are classified as invasive. The primary objective of our research was to achieve a comprehensive understanding of the current prevalence of two invasive parasite species, specifically the haematophagous abomasal nematode Ashworthius sidemi and the giant liver fluke Fascioloides magna, and to uncover their impact on wild ruminant populations. For these purposes, faecal samples of wild ruminants were collected from various locations across the Czech Republic between 2022 and 2024. These included 306 samples of roe deer (Capreolus capreolus) from 27 localities, 347 samples of red deer (Cervus elaphus) from 28 localities, 90 samples of fallow deer (Dama dama) from 10 localities, and 54 samples of mouflon (Ovis musimon) from 5 localities. To identify the selected parasite species at the molecular level, sensitive diagnostic methods based on real-time PCR with TaqMan probes were implemented for specific detection of their DNA directly in animal faeces. According to the findings, A. sidemi and F. magna are still present and spreading among ruminants in game reserves and hunting grounds in the Czech Republic. The red deer has been found to be a major carrier of an invasive nematode A. sidemi, which has been found in 89% of the areas where these animals were studied. Moreover, the giant liver fluke F. magna continues to pose a possible threat in the Central and South Bohemian Regions, particularly to domestic ruminants whose pastures may be contaminated by free-ranging game.
ER  -

ŠKORPÍKOVÁ, Lucie; Jana ILGOVÁ; Jaroslav VADLEJCH; Jan MAGDÁLEK; Radim PLHAL; Jakub DRIMAJ; Ondřej MIKULKA; Martin KAŠNÝ a Nikol RESLOVÁ. Utilizing direct molecular methods for screening of invasive helminth species in wild ruminants in the Czech Republic. In \textit{28TH HELMINTHOLOGICAL DAYS}. 2024. ISBN~978-80-11-05478-6.

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