LAMP-based electrochemical platform for monitoring HPV genome
integration at the mRNA level associated with higher risk of
cervical cancer progression

J 2024

LAMP-based electrochemical platform for monitoring HPV genome integration at the mRNA level associated with higher risk of cervical cancer progression

IZADI, Nasim; Johana STRMISKOVÁ; Milan ANTON; Jitka HAUSNEROVÁ; Martin BARTOŠÍK et al.

Základní údaje

Originální název

LAMP-based electrochemical platform for monitoring HPV genome integration at the mRNA level associated with higher risk of cervical cancer progression

Autoři

IZADI, Nasim; Johana STRMISKOVÁ; Milan ANTON; Jitka HAUSNEROVÁ a Martin BARTOŠÍK

Vydání

Journal of Medical Virology, Hoboken, Wiley, 2024, 0146-6615

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

20602 Medical laboratory technology

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

webová stránka časopisu

Impakt faktor

Impact factor: 4.600

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/24:00137358

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

cervical cancer; electrochemistry; HPV integration; human papillomavirus; RT‐LAMP

Štítky

14110230, 14110240, 143160, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 13. 2. 2025 10:04, Mgr. Johana Strmisková

Anotace

V originále

Human papillomaviruses (HPVs) represent a diverse group of double-stranded DNA viruses associated with various types of cancers, notably cervical cancer. High-risk types of HPVs exhibit their oncogenic potential through the integration of their DNA into the host genome. This integration event contributes significantly to genomic instability and the progression of malignancy. However, traditional detection methods, such as immunohistochemistry or PCR-based assays, face inherent challenges, and thus alternative tools are being developed to fasten and simplify the analysis. Our study introduces an innovative biosensing platform that combines loop-mediated amplification with electrochemical (EC) analysis for the specific detection of HPV16 integration. By targeting key elements like the E7 mRNA, a central player in HPV integration, and the E2 viral gene transcript lost upon integration, we show clear distinction between episomal and integrated forms of HPV16. Our EC data confirmed higher E7 expression in HPV16-positive cell lines having integrated forms of viral genome, while E2 expression was diminished in cells with fully integrated genomes. Moreover, we revealed distinct expression patterns in cervical tissue of patients, correlating well with digital droplet PCR, qRT-PCR, or immunohistochemical staining. Our platform thus offers insights into HPV integration in clinical samples and facilitates further advancements in cervical cancer research and diagnostics.

Návaznosti

LX22NPO5102, projekt VaV

Název: Národní ústav pro výzkum rakoviny (Akronym: NÚVR)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Národní ústav pro výzkum rakoviny, 5.1 EXCELES

90233, velká výzkumná infrastruktura

Název: BBMRI.cz IV

Citovat

IZADI, Nasim; Johana STRMISKOVÁ; Milan ANTON; Jitka HAUSNEROVÁ a Martin BARTOŠÍK. LAMP-based electrochemical platform for monitoring HPV genome integration at the mRNA level associated with higher risk of cervical cancer progression. Journal of Medical Virology. Hoboken: Wiley, 2024, roč. 96, č. 10, s. 1-10. ISSN 0146-6615. Dostupné z: https://doi.org/10.1002/jmv.70008.

@article{2444205,
   author = {Izadi, Nasim and Strmisková, Johana and Anton, Milan and Hausnerová, Jitka and Bartošík, Martin},
   article_location = {Hoboken},
   article_number = {10},
   doi = {https://doi.org/10.1002/jmv.70008},
   keywords = {cervical cancer; electrochemistry; HPV integration; human papillomavirus; RT‐LAMP},
   language = {eng},
   issn = {0146-6615},
   journal = {Journal of Medical Virology},
   title = {LAMP-based electrochemical platform for monitoring HPV genome integration at the mRNA level associated with higher risk of cervical cancer progression},
   url = {https://onlinelibrary.wiley.com/doi/10.1002/jmv.70008},
   volume = {96},
   year = {2024}
}

TY  - JOUR
ID  - 2444205
AU  - Izadi, Nasim - Strmisková, Johana - Anton, Milan - Hausnerová, Jitka - Bartošík, Martin
PY  - 2024
TI  - LAMP-based electrochemical platform for monitoring HPV genome integration at the mRNA level associated with higher risk of cervical cancer progression
JF  - Journal of Medical Virology
VL  - 96
IS  - 10
SP  - 1-10
EP  - 1-10
PB  - Wiley
SN  - 01466615
KW  - cervical cancer
KW  - electrochemistry
KW  - HPV integration
KW  - human papillomavirus
KW  - RT‐LAMP
UR  - https://onlinelibrary.wiley.com/doi/10.1002/jmv.70008
N2  - Human papillomaviruses (HPVs) represent a diverse group of double-stranded DNA viruses associated with various types of cancers, notably cervical cancer. High-risk types of HPVs exhibit their oncogenic potential through the integration of their DNA into the host genome. This integration event contributes significantly to genomic instability and the progression of malignancy. However, traditional detection methods, such as immunohistochemistry or PCR-based assays, face inherent challenges, and thus alternative tools are being developed to fasten and simplify the analysis. Our study introduces an innovative biosensing platform that combines loop-mediated amplification with electrochemical (EC) analysis for the specific detection of HPV16 integration. By targeting key elements like the E7 mRNA, a central player in HPV integration, and the E2 viral gene transcript lost upon integration, we show clear distinction between episomal and integrated forms of HPV16. Our EC data confirmed higher E7 expression in HPV16-positive cell lines having integrated forms of viral genome, while E2 expression was diminished in cells with fully integrated genomes. Moreover, we revealed distinct expression patterns in cervical tissue of patients, correlating well with digital droplet PCR, qRT-PCR, or immunohistochemical staining. Our platform thus offers insights into HPV integration in clinical samples and facilitates further advancements in cervical cancer research and diagnostics.
ER  -

IZADI, Nasim; Johana STRMISKOVÁ; Milan ANTON; Jitka HAUSNEROVÁ a Martin BARTOŠÍK. LAMP-based electrochemical platform for monitoring HPV genome integration at the mRNA level associated with higher risk of cervical cancer progression. \textit{Journal of Medical Virology}. Hoboken: Wiley, 2024, roč.~96, č.~10, s.~1-10. ISSN~0146-6615. Dostupné z: https://doi.org/10.1002/jmv.70008.

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