Phage Therapy Combined with Gum Karaya Hydrogels for Treatment
of Multi-resistant Pseudomonas aeruginosa Deep Wound Infection
in a Porcine Model

a 2024

Phage Therapy Combined with Gum Karaya Hydrogels for Treatment of Multi-resistant Pseudomonas aeruginosa Deep Wound Infection in a Porcine Model

VACEK, Lukáš; Dominika POLAŠTÍK KLEKNEROVÁ; Břetislav LIPOVÝ; Eva ČERNÁ; Jana BRTNÍKOVÁ et al.

Základní údaje

Originální název

Phage Therapy Combined with Gum Karaya Hydrogels for Treatment of Multi-resistant Pseudomonas aeruginosa Deep Wound Infection in a Porcine Model

Autoři

VACEK, Lukáš ; Dominika POLAŠTÍK KLEKNEROVÁ; Břetislav LIPOVÝ; Eva ČERNÁ; Jana BRTNÍKOVÁ; Edita JEKLOVÁ; Lenka LIŠKOVÁ; Roman PANTŮČEK ; Lucy VOJTOVÁ a Filip RŮŽIČKA

Vydání

International Biennial Pseudomonas Conference 2024, 2024

Další údaje

Jazyk

angličtina

Typ výsledku

Konferenční abstrakt

Obor

10606 Microbiology

Stát vydavatele

Dánsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14110/24:00139964

Organizační jednotka

Lékařská fakulta

Klíčová slova anglicky

porcine model; skin and soft tissue infections; SSTI; phage therapy; Pseudomonas

Příznaky

Mezinárodní význam

Změněno: 6. 12. 2024 15:44, Mgr. Lukáš Vacek, Ph.D.

Anotace

V originále

Background Gum karaya (GK) polysaccharide-based hydrogels keep a moist environment and stimulate wound healing. Its antimicrobial potential against multi-resistant Pseudomonas aeruginosa strains has been previously established. Supplementation of GK with phage preparations can increase its antimicrobial potential.   Methods A total of six fully immunocompetent pigs, each with twenty 5 x 5 cm skin defects, were used. The multi-resistant P. aeruginosa strain FF2 was applied after surgical excisions. On Days 4, 7, 11, and 14 after infection, tissue samples of wounds were taken for microbiological evaluation. The wounds of four pigs were treated using GK hydrogels supplemented with phages Pbunavirus (MB501) and Phikmvvirus (LUZ19) at concentrations of 10^9 PFU per wound. Two pigs were left untreated to monitor the progress of infection.   Results Three days after infection, wounds showed a bacterial load of 7.8 ± 0.4 log CFU/g of tissue. During the experiment, untreated pigs exhibited a decrease in bacterial numbers in wounds to 6.9 ± 0.4 log CFU/g (1.0 log CFU/g difference). The decrease in wounds of treated pigs was to 5.1 ± 0.8 log CFU/g of tissue (2.7 log CFU/g difference). The statistical evaluation showed a significant decrease in the number of bacteria (p < 0.01). Conclusion The study shows a synergistic effect of GK and phage therapy, resulting in a significant reduction in bacterial loads within ten days of treatment. No adverse effects of the GK hydrogels of phage preparations were observed during the study. Therefore, this treatment offers a promising avenue for future research and clinical applications.

Návaznosti

NU20-05-00166, projekt VaV

Název: Nové přístupy k hojení infikovaných ran pomocí hydrogelů z přírodní pryskyřice Gum Karaya modifikovaných inovativními komponenty cílenými na multirezistentní kmeny bakterií (Akronym: NPHIRPHGK)

Investor: Ministerstvo zdravotnictví ČR, Nové přístupy k hojení infikovaných ran pomocí hydrogelů z přírodní pryskyřice Gum Karaya modifikovaných inovativními komponenty cílenými na multirezistentní kmeny bakterií, Podprogram 1 - standardní

Citovat

VACEK, Lukáš; Dominika POLAŠTÍK KLEKNEROVÁ; Břetislav LIPOVÝ; Eva ČERNÁ; Jana BRTNÍKOVÁ; Edita JEKLOVÁ; Lenka LIŠKOVÁ; Roman PANTŮČEK; Lucy VOJTOVÁ a Filip RŮŽIČKA. Phage Therapy Combined with Gum Karaya Hydrogels for Treatment of Multi-resistant Pseudomonas aeruginosa Deep Wound Infection in a Porcine Model. In International Biennial Pseudomonas Conference 2024. 2024.

@proceedings{2447657,
   author = {Vacek, Lukáš and Polaštík Kleknerová, Dominika and Lipový, Břetislav and Černá, Eva and Brtníková, Jana and Jeklová, Edita and Lišková, Lenka and Pantůček, Roman and Vojtová, Lucy and Růžička, Filip},
   booktitle = {International Biennial Pseudomonas Conference 2024},
   keywords = {porcine model; skin and soft tissue infections; SSTI; phage therapy; Pseudomonas},
   language = {eng},
   title = {Phage Therapy Combined with Gum Karaya Hydrogels for Treatment of Multi-resistant Pseudomonas aeruginosa Deep Wound Infection in a Porcine Model},
   url = {https://microbiologysociety.org/event/society-events-and-meetings/international-biennial-pseudomonas-conference.html},
   year = {2024}
}

TY  - CONF
ID  - 2447657
AU  - Vacek, Lukáš - Polaštík Kleknerová, Dominika - Lipový, Břetislav - Černá, Eva - Brtníková, Jana - Jeklová, Edita - Lišková, Lenka - Pantůček, Roman - Vojtová, Lucy - Růžička, Filip
PY  - 2024
TI  - Phage Therapy Combined with Gum Karaya Hydrogels for Treatment of Multi-resistant Pseudomonas aeruginosa Deep Wound Infection in a Porcine Model
KW  - porcine model
KW  - skin and soft tissue infections
KW  - SSTI
KW  - phage therapy
KW  - Pseudomonas
UR  - https://microbiologysociety.org/event/society-events-and-meetings/international-biennial-pseudomonas-conference.html
N2  - Background Gum karaya (GK) polysaccharide-based hydrogels keep a moist environment and stimulate wound healing. Its antimicrobial potential against multi-resistant Pseudomonas aeruginosa strains has been previously established. Supplementation of GK with phage preparations can increase its antimicrobial potential.   Methods A total of six fully immunocompetent pigs, each with twenty 5 x 5 cm skin defects, were used. The multi-resistant P. aeruginosa strain FF2 was applied after surgical excisions. On Days 4, 7, 11, and 14 after infection, tissue samples of wounds were taken for microbiological evaluation. The wounds of four pigs were treated using GK hydrogels supplemented with phages Pbunavirus (MB501) and Phikmvvirus (LUZ19) at concentrations of 10^9 PFU per wound. Two pigs were left untreated to monitor the progress of infection.   Results Three days after infection, wounds showed a bacterial load of 7.8 ± 0.4 log CFU/g of tissue. During the experiment, untreated pigs exhibited a decrease in bacterial numbers in wounds to 6.9 ± 0.4 log CFU/g (1.0 log CFU/g difference). The decrease in wounds of treated pigs was to 5.1 ± 0.8 log CFU/g of tissue (2.7 log CFU/g difference). The statistical evaluation showed a significant decrease in the number of bacteria (p < 0.01). Conclusion The study shows a synergistic effect of GK and phage therapy, resulting in a significant reduction in bacterial loads within ten days of treatment. No adverse effects of the GK hydrogels of phage preparations were observed during the study. Therefore, this treatment offers a promising avenue for future research and clinical applications.
ER  -

VACEK, Lukáš; Dominika POLAŠTÍK KLEKNEROVÁ; Břetislav LIPOVÝ; Eva ČERNÁ; Jana BRTNÍKOVÁ; Edita JEKLOVÁ; Lenka LIŠKOVÁ; Roman PANTŮČEK; Lucy VOJTOVÁ a Filip RŮŽIČKA. Phage Therapy Combined with Gum Karaya Hydrogels for Treatment of Multi-resistant Pseudomonas aeruginosa Deep Wound Infection in a Porcine Model. In \textit{International Biennial Pseudomonas Conference 2024}. 2024.

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