2024
INVESTIGATING THE HONEY BEE APOLIPOPHORINS
MARCINIAK, Jacek; Jana HURYCHOVÁ; Sara ŠREIBR; Pavel HYRŠL; James B. BURRITT et al.Základní údaje
Originální název
INVESTIGATING THE HONEY BEE APOLIPOPHORINS
Název česky
INVESTIGATING THE HONEY BEE APOLIPOPHORINS
Autoři
Vydání
EurBee 10 - 10th Congress of Apidology, 2024
Další údaje
Jazyk
angličtina
Typ výsledku
Konferenční abstrakt
Obor
10616 Entomology
Stát vydavatele
Estonsko
Utajení
není předmětem státního či obchodního tajemství
Označené pro přenos do RIV
Ano
Kód RIV
RIV/00216224:14310/24:00139783
Organizační jednotka
Přírodovědecká fakulta
ISSN
Klíčová slova česky
Apis mellifera; apolipoforin; metabolismus; imunitní systém
Klíčová slova anglicky
Apis mellifera; apolipophorin; metabolism; immune system
Příznaky
Mezinárodní význam
Změněno: 3. 3. 2025 21:40, Mgr. Jacek Marciniak
V originále
Apolipophorins (ApoLp) are a group of insect proteins belonging to the large lipid transfer protein superfamily. They ensure the transport of lipids, carbohydrates, and other hydrophobic compounds by forming lipophorin particles. Lipophorins are assembled in the fat body and after their release to the hemolymph operate as reusable lipid shuttles between the fat body and target tissues such as flight muscles or epidermis. Our research focuses on the ApoLp I and its precursor, the ApoLp II/I. Based on shared functional domains with another pleiotropic protein, vitellogenin, we believe that these proteins may substitute vitellogenin in some of its functions and that their research in the context of immune responses and physiological adaptations may bring new insights into honey bee biology. So far, in-house monoclonal antibodies have been deployed to determine the variation of ApoLp I in different contexts using the western blot technique. We observed that the ApoLp I level varies through the seasons and shows a reversed trend compared to vitellogenin. We also observed increased ApoLp I levels in bacteria-infected bees. These findings support our hypothesis that apolipophorins are multifunctional proteins playing a crucial role in honey bee physiology and immunity. This will be followed by tandem mass spectrometry-based techniques to determine interacting partners of ApoLp I and knock-down experiments to shed more light on its function.
Česky
Apolipophorins (ApoLp) are a group of insect proteins belonging to the large lipid transfer protein superfamily. They ensure the transport of lipids, carbohydrates, and other hydrophobic compounds by forming lipophorin particles. Lipophorins are assembled in the fat body and after their release to the hemolymph operate as reusable lipid shuttles between the fat body and target tissues such as flight muscles or epidermis. Our research focuses on the ApoLp I and its precursor, the ApoLp II/I. Based on shared functional domains with another pleiotropic protein, vitellogenin, we believe that these proteins may substitute vitellogenin in some of its functions and that their research in the context of immune responses and physiological adaptations may bring new insights into honey bee biology. So far, in-house monoclonal antibodies have been deployed to determine the variation of ApoLp I in different contexts using the western blot technique. We observed that the ApoLp I level varies through the seasons and shows a reversed trend compared to vitellogenin. We also observed increased ApoLp I levels in bacteria-infected bees. These findings support our hypothesis that apolipophorins are multifunctional proteins playing a crucial role in honey bee physiology and immunity. This will be followed by tandem mass spectrometry-based techniques to determine interacting partners of ApoLp I and knock-down experiments to shed more light on its function.
Návaznosti
| QK1910286, projekt VaV |
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