Transcriptional and phenotypical alterations associated with a
gradual benzo[a]pyrene-induced transition of human bronchial
epithelial cells into mesenchymal-like cells

J 2024

Transcriptional and phenotypical alterations associated with a gradual benzo[a]pyrene-induced transition of human bronchial epithelial cells into mesenchymal-like cells

HÝŽĎALOVÁ, Martina; Jiřina PROCHÁZKOVÁ; Nicol STRAKOVÁ; Kateřina PĚNČÍKOVÁ; Simona STRAPÁČOVÁ et. al.

Základní údaje

Originální název

Transcriptional and phenotypical alterations associated with a gradual benzo[a]pyrene-induced transition of human bronchial epithelial cells into mesenchymal-like cells

Autoři

HÝŽĎALOVÁ, Martina; Jiřina PROCHÁZKOVÁ; Nicol STRAKOVÁ; Kateřina PĚNČÍKOVÁ; Simona STRAPÁČOVÁ; Jana SLOVÁČKOVÁ (203 Česká republika, domácí); Simona KAJABOVÁ (703 Slovensko, domácí); Helena LÍBALOVÁ; Jan TOPINKA; Markéta KABÁTKOVÁ; Jan VONDRÁČEK; Steen MOLLERUP a Miroslav MACHALA

Vydání

Environmental Toxicology and Pharmacology, Amsterdam, Elsevier Science B.V. 2024, 1382-6689

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30108 Toxicology

Stát vydavatele

Nizozemské království

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 4.200 v roce 2023

Kód RIV

RIV/00216224:14110/24:00137695

Organizační jednotka

Lékařská fakulta

DOI

http://dx.doi.org/10.1016/j.etap.2024.104424

UT WoS

001215550700001

EID Scopus

2-s2.0-85189083043

Klíčová slova anglicky

Benzo[a]pyrene; Aryl hydrocarbon receptor; 2 3 7 8-tetrachlorodibenzo-p-dioxin; Epithelial-mesenchymal transition; Human bronchial epithelial cells

Štítky

14110517, podil, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 27. 3. 2025 09:09, Mgr. Tereza Miškechová

Anotace

V originále

The role of benzo[a]pyrene (BaP), a prominent genotoxic carcinogen and aryl hydrocarbon receptor (AhR) ligand, in tumor progression remains poorly characterized. We investigated the impact of BaP on the process of epithelial-mesenchymal transition (EMT) in normal human bronchial epithelial HBEC-12KT cells. Early morphological changes after 2-week exposure were accompanied with induction of SERPINB2, IL1, CDKN1A/p21 (linked with cell cycle delay) and chemokine CXCL5. After 8-week exposure, induction of cell migration and EMT-related pattern of markers/regulators led to induction of further pro-inflammatory cytokines or non-canonical Wnt pathway ligand WNT5A. This trend of up-regulation of pro-inflammatory genes and non-canonical Wnt pathway constituents was observed also in the BaP-transformed HBEC-12KT-B1 cells. In general, transcriptional effects of BaP differed from those of TGFβ1, a prototypical EMT inducer, or a model non-genotoxic AhR ligand, TCDD. Carcinogenic polycyclic aromatic hydrocarbons could thus induce a unique set of molecular changes linked with EMT and cancer progression.

Citovat

HÝŽĎALOVÁ, Martina; Jiřina PROCHÁZKOVÁ; Nicol STRAKOVÁ; Kateřina PĚNČÍKOVÁ; Simona STRAPÁČOVÁ; Jana SLOVÁČKOVÁ; Simona KAJABOVÁ; Helena LÍBALOVÁ; Jan TOPINKA; Markéta KABÁTKOVÁ; Jan VONDRÁČEK; Steen MOLLERUP a Miroslav MACHALA. Transcriptional and phenotypical alterations associated with a gradual benzo[a]pyrene-induced transition of human bronchial epithelial cells into mesenchymal-like cells. Environmental Toxicology and Pharmacology. Amsterdam: Elsevier Science B.V., 2024, roč. 107, April 2024, s. 104424-104436. ISSN 1382-6689. Dostupné z: https://dx.doi.org/10.1016/j.etap.2024.104424.

@article{2453318,
   author = {Hýžďalová, Martina and Procházková, Jiřina and Straková, Nicol and Pěnčíková, Kateřina and Strapáčová, Simona and Slováčková, Jana and Kajabová, Simona and Líbalová, Helena and Topinka, Jan and Kabátková, Markéta and Vondráček, Jan and Mollerup, Steen and Machala, Miroslav},
   article_location = {Amsterdam},
   article_number = {April 2024},
   doi = {http://dx.doi.org/10.1016/j.etap.2024.104424},
   keywords = {Benzo[a]pyrene; Aryl hydrocarbon receptor; 2 3 7 8-tetrachlorodibenzo-p-dioxin; Epithelial-mesenchymal transition; Human bronchial epithelial cells},
   language = {eng},
   issn = {1382-6689},
   journal = {Environmental Toxicology and Pharmacology},
   title = {Transcriptional and phenotypical alterations associated with a gradual benzo[a]pyrene-induced transition of human bronchial epithelial cells into mesenchymal-like cells},
   url = {https://www.sciencedirect.com/science/article/pii/S1382668924000644},
   volume = {107},
   year = {2024}
}

TY  - JOUR
ID  - 2453318
AU  - Hýžďalová, Martina - Procházková, Jiřina - Straková, Nicol - Pěnčíková, Kateřina - Strapáčová, Simona - Slováčková, Jana - Kajabová, Simona - Líbalová, Helena - Topinka, Jan - Kabátková, Markéta - Vondráček, Jan - Mollerup, Steen - Machala, Miroslav
PY  - 2024
TI  - Transcriptional and phenotypical alterations associated with a gradual benzo[a]pyrene-induced transition of human bronchial epithelial cells into mesenchymal-like cells
JF  - Environmental Toxicology and Pharmacology
VL  - 107
IS  - April 2024
SP  - 104424
EP  - 104424
PB  - Elsevier Science B.V.
SN  - 13826689
KW  - Benzo[a]pyrene
KW  - Aryl hydrocarbon receptor
KW  - 2 3 7 8-tetrachlorodibenzo-p-dioxin
KW  - Epithelial-mesenchymal transition
KW  - Human bronchial epithelial cells
UR  - https://www.sciencedirect.com/science/article/pii/S1382668924000644
N2  - The role of benzo[a]pyrene (BaP), a prominent genotoxic carcinogen and aryl hydrocarbon receptor (AhR) ligand, in tumor progression remains poorly characterized. We investigated the impact of BaP on the process of epithelial-mesenchymal transition (EMT) in normal human bronchial epithelial HBEC-12KT cells. Early morphological changes after 2-week exposure were accompanied with induction of SERPINB2, IL1, CDKN1A/p21 (linked with cell cycle delay) and chemokine CXCL5. After 8-week exposure, induction of cell migration and EMT-related pattern of markers/regulators led to induction of further pro-inflammatory cytokines or non-canonical Wnt pathway ligand WNT5A. This trend of up-regulation of pro-inflammatory genes and non-canonical Wnt pathway constituents was observed also in the BaP-transformed HBEC-12KT-B1 cells. In general, transcriptional effects of BaP differed from those of TGFβ1, a prototypical EMT inducer, or a model non-genotoxic AhR ligand, TCDD. Carcinogenic polycyclic aromatic hydrocarbons could thus induce a unique set of molecular changes linked with EMT and cancer progression.
ER  -

HÝŽĎALOVÁ, Martina; Jiřina PROCHÁZKOVÁ; Nicol STRAKOVÁ; Kateřina PĚNČÍKOVÁ; Simona STRAPÁČOVÁ; Jana SLOVÁČKOVÁ; Simona KAJABOVÁ; Helena LÍBALOVÁ; Jan TOPINKA; Markéta KABÁTKOVÁ; Jan VONDRÁČEK; Steen MOLLERUP a Miroslav MACHALA. Transcriptional and phenotypical alterations associated with a gradual benzo[a]pyrene-induced transition of human bronchial epithelial cells into mesenchymal-like cells. \textit{Environmental Toxicology and Pharmacology}. Amsterdam: Elsevier Science B.V., 2024, roč.~107, April 2024, s.~104424-104436. ISSN~1382-6689. Dostupné z: https://dx.doi.org/10.1016/j.etap.2024.104424.

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