Phage training: Enhancing bacteriophage efficacy in
antibiotic-infused environments

a 2024

Phage training: Enhancing bacteriophage efficacy in antibiotic-infused environments

KUČEROVÁ, Eliška; Barbora KOSTELNÍKOVÁ; Jana NOVOTNÁ; Roman PANTŮČEK; Tibor BOTKA et al.

Základní údaje

Originální název

Phage training: Enhancing bacteriophage efficacy in antibiotic-infused environments

Autoři

KUČEROVÁ, Eliška; Barbora KOSTELNÍKOVÁ; Jana NOVOTNÁ; Roman PANTŮČEK a Tibor BOTKA

Vydání

Meeting of the National Institute of Virology and Bacteriology (NIVB) in Kutná Hora 2024, Czech Chemical Society Symposium Series 22 (6) 199-276, 2024

Další údaje

Typ výsledku

Konferenční abstrakt

Utajení

není předmětem státního či obchodního tajemství

Označené pro přenos do RIV

Organizační jednotka

Přírodovědecká fakulta

ISSN

Příznaky

Recenzováno

Změněno: 28. 12. 2024 14:53, prof. RNDr. Roman Pantůček, Ph.D.

Anotace

V originále

Viruses of microbes (bacteriophages) are an ubiquitous entity present in our environment. From the beginning of the previous century humankind looking for their utilization in medicine. The medicinal approach counts only with strictly lytic phages, which lyses the target pathogenic bacteria without adverse effects on the human host. So the second most important criterium is the phage ability to lyse the selected bacterial strain. Unfortunately the phage banks may not always contain the right phages and the isolation of the new lytic phages could be tedious and not fast enough for the patient. Another obstacle is that the lytic ability of phage could change during the therapeutic process, because of emergence of the phage resistance. Phage training could be a solution for these situations. It is a process of adaptive laboratory evolution, where the phages should acquire the ability to lyse the selected bacteria. Our training approach is based on Phage-Antibiotic Synergy effect (PAS). The phage-antibiotic interactions could lead to an antagonism or synergy, where is the bacteria more efficiently eradicated. Both of these situation were used for the training of therapeutic phages. Resulting phages were tested for their efficiacy against the parental strain of S. Aureus and the best clones were isolated and sequenced by Nanopore technology. Genome changes were evaluated in comparison to the parental phage. Also the evolved bacteria were isolated, tested for their identity and resistance to the parental phage. The identifiaction of these changes could reveal a new insights into the phage-antibiotic function and evolution.

Návaznosti

LX22NPO5103, projekt VaV

Název: Národní institut virologie a bakteriologie (Akronym: NIVB)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Národní institut virologie a bakteriologie, 5.1 EXCELES

Citovat

KUČEROVÁ, Eliška; Barbora KOSTELNÍKOVÁ; Jana NOVOTNÁ; Roman PANTŮČEK a Tibor BOTKA. Phage training: Enhancing bacteriophage efficacy in antibiotic-infused environments. In Meeting of the National Institute of Virology and Bacteriology (NIVB) in Kutná Hora 2024, Czech Chemical Society Symposium Series 22 (6) 199-276. 2024. ISSN 2336-7202.

@proceedings{2461877,
   author = {Kučerová, Eliška and Kostelníková, Barbora and Novotná, Jana and Pantůček, Roman and Botka, Tibor},
   booktitle = {Meeting of the National Institute of Virology and Bacteriology (NIVB) in Kutná Hora 2024, Czech Chemical Society Symposium Series 22 (6) 199-276},
   title = {Phage training: Enhancing bacteriophage efficacy in antibiotic-infused environments},
   year = {2024}
}

TY  - CONF
ID  - 2461877
AU  - Kučerová, Eliška - Kostelníková, Barbora - Novotná, Jana - Pantůček, Roman - Botka, Tibor
PY  - 2024
TI  - Phage training: Enhancing bacteriophage efficacy in antibiotic-infused environments
N2  - Viruses of microbes (bacteriophages) are an ubiquitous entity present in our environment. From the beginning of the previous century humankind looking for their utilization in medicine. The medicinal approach counts only with strictly lytic phages, which lyses the target pathogenic bacteria without adverse effects on the human host. So the second most important criterium is the phage ability to lyse the selected bacterial strain. Unfortunately the phage banks may not always contain the right phages and the isolation of the new lytic phages could be tedious and not fast enough for the patient. Another obstacle is that the lytic ability of phage could change during the therapeutic process, because of emergence of the phage resistance. Phage training could be a solution for these situations. It is a process of adaptive laboratory evolution, where the phages should acquire the ability to lyse the selected bacteria. Our training approach is based on Phage-Antibiotic Synergy effect (PAS). The phage-antibiotic interactions could lead to an antagonism or synergy, where is the bacteria more efficiently eradicated. Both of these situation were used for the training of therapeutic phages. Resulting phages were tested for their efficiacy against the parental strain of S. Aureus and the best clones were isolated and sequenced by Nanopore technology. Genome changes were evaluated in comparison to the parental phage. Also the evolved bacteria were isolated, tested for their identity and resistance to the parental phage. The identifiaction of these changes could reveal a new insights into the phage-antibiotic function and evolution.
ER  -

KUČEROVÁ, Eliška; Barbora KOSTELNÍKOVÁ; Jana NOVOTNÁ; Roman PANTŮČEK a Tibor BOTKA. Phage training: Enhancing bacteriophage efficacy in antibiotic-infused environments. In \textit{Meeting of the National Institute of Virology and Bacteriology (NIVB) in Kutná Hora 2024, Czech Chemical Society Symposium Series 22 (6) 199-276}. 2024. ISSN~2336-7202.

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