Fabrication of novel PHB-liposome nanoparticles and study of
their toxicity in vitro

J 2019

Fabrication of novel PHB-liposome nanoparticles and study of their toxicity in vitro

BOKROVA, Jitka; Ivana MAROVA; Petra MATOUSKOVA a Renata PAVELKOVA

Základní údaje

Originální název

Fabrication of novel PHB-liposome nanoparticles and study of their toxicity in vitro

Autoři

BOKROVA, Jitka; Ivana MAROVA; Petra MATOUSKOVA a Renata PAVELKOVA

Vydání

Journal of Nanoparticle Research, DORDRECHT, SPRINGER, 2019, 1388-0764

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10400 1.4 Chemical sciences

Stát vydavatele

Nizozemské království

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 2.132

Kód RIV

RIV/00216224:90043/19:00138364

Organizační jednotka

CIISB

DOI

https://doi.org/10.1007/s11051-019-4484-7

UT WoS

000460518700001

EID Scopus

2-s2.0-85063122981

Klíčová slova anglicky

Nanoparticles; Liposomes; PHB; Cytotoxicity; Nanotoxicity; Keratinocytes; Health effects

Štítky

CF CRYO, ne MU, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 9. 1. 2025 08:44, Mgr. Eva Dubská

Anotace

V originále

Liposomes represent a tool used in cosmetics and for drug delivery due to their small size, biocompatibility, and ability to house both hydrophobic and hydrophilic molecules. However, under storage, there is a tendency for liposomes to release encapsulated components. In order to enhance their stability, combination with biodegradable polymers may be a promising option. Bacterial polymer poly(3-hydroxybutyrate) (PHB) is one such material that may contribute liposome structure, as it is also biocompatible and biodegradable and promotes controlled release of active agents in the skin. Nanoparticles consisting of PHB and phospholipids can be very attractive as biomaterials for application in cosmetics. In this work, novel combined PHB-liposome nanoparticles were synthesized and characterized, and their biocompatability with mammalian cells was assessed in vitro. Incorporation of PHB into liposome particles led to preservation of small size (123.8 +/- 0.9nm; evaluated by DLS) and colloidal stability of particles for 2months in water environment. Cytotoxicity studies using MTT test with two different skin keratinocytes cell lines (HEK and HaCaT) showed significant dose-dependent differences in cell viability after exposure to liposome and PHB liposome particles in vitro. However, these PHB-liposomes were not found toxic both for HEK and HaCaT cells. Regarding to natural UV-scattering effect of PHB, we suggest that newly fabricated combined liposome-PHB particles could exhibit added value as carriers of active substances for topical applications. However, further study is necessary in order to reveal interactions between this new type of particle and human cells.

Návaznosti

90043, velká výzkumná infrastruktura

Název: CIISB

Citovat

BOKROVA, Jitka; Ivana MAROVA; Petra MATOUSKOVA a Renata PAVELKOVA. Fabrication of novel PHB-liposome nanoparticles and study of their toxicity in vitro. Journal of Nanoparticle Research. DORDRECHT: SPRINGER, 2019, roč. 21, č. 3, s. 1-12. ISSN 1388-0764. Dostupné z: https://doi.org/10.1007/s11051-019-4484-7.

@article{2466158,
   author = {Bokrova, Jitka and Marova, Ivana and Matouskova, Petra and Pavelkova, Renata},
   article_location = {DORDRECHT},
   article_number = {3},
   doi = {https://doi.org/10.1007/s11051-019-4484-7},
   keywords = {Nanoparticles; Liposomes; PHB; Cytotoxicity; Nanotoxicity; Keratinocytes; Health effects},
   language = {eng},
   issn = {1388-0764},
   journal = {Journal of Nanoparticle Research},
   title = {Fabrication of novel PHB-liposome nanoparticles and study of their toxicity in vitro},
   url = {https://link.springer.com/article/10.1007/s11051-019-4484-7},
   volume = {21},
   year = {2019}
}

TY  - JOUR
ID  - 2466158
AU  - Bokrova, Jitka - Marova, Ivana - Matouskova, Petra - Pavelkova, Renata
PY  - 2019
TI  - Fabrication of novel PHB-liposome nanoparticles and study of their toxicity in vitro
JF  - Journal of Nanoparticle Research
VL  - 21
IS  - 3
SP  - 1-12
EP  - 1-12
PB  - SPRINGER
SN  - 13880764
KW  - Nanoparticles
KW  - Liposomes
KW  - PHB
KW  - Cytotoxicity
KW  - Nanotoxicity
KW  - Keratinocytes
KW  - Health effects
UR  - https://link.springer.com/article/10.1007/s11051-019-4484-7
N2  - Liposomes represent a tool used in cosmetics and for drug delivery due to their small size, biocompatibility, and ability to house both hydrophobic and hydrophilic molecules. However, under storage, there is a tendency for liposomes to release encapsulated components. In order to enhance their stability, combination with biodegradable polymers may be a promising option. Bacterial polymer poly(3-hydroxybutyrate) (PHB) is one such material that may contribute liposome structure, as it is also biocompatible and biodegradable and promotes controlled release of active agents in the skin. Nanoparticles consisting of PHB and phospholipids can be very attractive as biomaterials for application in cosmetics. In this work, novel combined PHB-liposome nanoparticles were synthesized and characterized, and their biocompatability with mammalian cells was assessed in vitro. Incorporation of PHB into liposome particles led to preservation of small size (123.8 +/- 0.9nm; evaluated by DLS) and colloidal stability of particles for 2months in water environment. Cytotoxicity studies using MTT test with two different skin keratinocytes cell lines (HEK and HaCaT) showed significant dose-dependent differences in cell viability after exposure to liposome and PHB liposome particles in vitro. However, these PHB-liposomes were not found toxic both for HEK and HaCaT cells. Regarding to natural UV-scattering effect of PHB, we suggest that newly fabricated combined liposome-PHB particles could exhibit added value as carriers of active substances for topical applications. However, further study is necessary in order to reveal interactions between this new type of particle and human cells.
ER  -

BOKROVA, Jitka; Ivana MAROVA; Petra MATOUSKOVA a Renata PAVELKOVA. Fabrication of novel PHB-liposome nanoparticles and study of their toxicity in vitro. \textit{Journal of Nanoparticle Research}. DORDRECHT: SPRINGER, 2019, roč.~21, č.~3, s.~1-12. ISSN~1388-0764. Dostupné z: https://doi.org/10.1007/s11051-019-4484-7.

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