Asymptomatic carriage and molecular characterization of
Staphylococcus aureus in pre-clinical and clinical medical
students

J 2025

Asymptomatic carriage and molecular characterization of Staphylococcus aureus in pre-clinical and clinical medical students

BRODÍKOVÁ, Kristýna; Bohdana REZKOVÁ; Ivana KOLÁČKOVÁ a Renata KARPÍŠKOVÁ

Základní údaje

Originální název

Asymptomatic carriage and molecular characterization of Staphylococcus aureus in pre-clinical and clinical medical students

Autoři

BRODÍKOVÁ, Kristýna; Bohdana REZKOVÁ ; Ivana KOLÁČKOVÁ a Renata KARPÍŠKOVÁ

Vydání

FOLIA MICROBIOLOGICA, DORDRECHT, SPRINGER, 2025, 0015-5632

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10606 Microbiology

Stát vydavatele

Nizozemské království

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 3.100 v roce 2024

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14110/25:00144718

Organizační jednotka

Lékařská fakulta

Klíčová slova anglicky

Staphylococcus aureus; MRSA; Medical students; Spa type; Antibiotic resistance; Virulence genes

Štítky

14110525, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 7. 4. 2025 10:39, Mgr. Tereza Miškechová

Anotace

V originále

Medical students are exposed to the hospital environment and patients during their studies, increasing the risk of exposure to virulent and antibiotic-resistant isolates of Staphylococcus aureus. The aim of the study is to determine the prevalence of Staphylococcus aureus among medical students who have varying levels of exposure to the hospital environment to provide valuable insights into the risk of colonization and transmission. Nasal swabs and fingerprints were obtained and cultured on a selective medium for staphylococci. The obtained isolates were confirmed as methicillin-sensitive S. aureus (MSSA) or methicillin-resistant (MRSA) using PCR. Antibiotic resistance, the presence of virulence genes including enterotoxin encoding genes, and spa typing were performed. Among pre-clinical students, MSSA was detected on the nose in 45.2% and on the fingerprints in 10.6% of the participants. Among clinical students, MSSA was detected on the nose in 42.0% and on the fingerprints in 25.4%. Only one MRSA isolate was obtained. Genes seg and sei were the most frequently detected in both student groups, with their presence in over 40% of isolates among clinical students. The eta and etb genes were mainly detected from the nose in both student groups. In pre-clinical students, S. aureus carrying eta gene occurred in 6.4% and etb in 8.5%. In clinical students, the occurrence was 5.1% for eta and 8.5% for etb. The tst gene was identified only in the nose and fingerprints of the clinical student group. The most frequently observed resistance was to clindamycin and erythromycin. In total 58 different spa types were identified. High rates of asymptomatic MSSA carriage were observed in both groups of medical students. Detected MSSA strains showed a high degree of genetic variability, with a number of them carrying the virulence and antibiotic resistance genes. Although students do not exhibit increased risk to their patient’s, increased hygiene is required in asymptomatic carriage personnel. The overall prevalence of MRSA was low, with a minimal risk of spread.

Návaznosti

NU23-09-00488, projekt VaV

Název: Epidemiologická a genetická analýza meticilin-rezistentních kmenů Staphylococcus aureus dle konceptu WHO “One Health”

Investor: Ministerstvo zdravotnictví ČR, Epidemiologická a genetická analýza meticillin rezistentních kmenů Staphylococcus aureus dle konceptu WHO "One health", Podprogram 1 - standardní

Citovat

BRODÍKOVÁ, Kristýna; Bohdana REZKOVÁ; Ivana KOLÁČKOVÁ a Renata KARPÍŠKOVÁ. Asymptomatic carriage and molecular characterization of Staphylococcus aureus in pre-clinical and clinical medical students. FOLIA MICROBIOLOGICA. DORDRECHT: SPRINGER, 2025, roč. 70, č. 1, s. 241-248. ISSN 0015-5632. Dostupné z: https://doi.org/10.1007/s12223-024-01237-1.

@article{2467521,
   author = {Brodíková, Kristýna and Rezková, Bohdana and Koláčková, Ivana and Karpíšková, Renata},
   article_location = {DORDRECHT},
   article_number = {1},
   doi = {https://doi.org/10.1007/s12223-024-01237-1},
   keywords = {Staphylococcus aureus; MRSA; Medical students; Spa type; Antibiotic resistance; Virulence genes},
   language = {eng},
   issn = {0015-5632},
   journal = {FOLIA MICROBIOLOGICA},
   title = {Asymptomatic carriage and molecular characterization of Staphylococcus aureus in pre-clinical and clinical medical students},
   url = {https://link.springer.com/article/10.1007/s12223-024-01237-1},
   volume = {70},
   year = {2025}
}

TY  - JOUR
ID  - 2467521
AU  - Brodíková, Kristýna - Rezková, Bohdana - Koláčková, Ivana - Karpíšková, Renata
PY  - 2025
TI  - Asymptomatic carriage and molecular characterization of Staphylococcus aureus in pre-clinical and clinical medical students
JF  - FOLIA MICROBIOLOGICA
VL  - 70
IS  - 1
SP  - 241-248
EP  - 241-248
PB  - SPRINGER
SN  - 00155632
KW  - Staphylococcus aureus
KW  - MRSA
KW  - Medical students
KW  - Spa type
KW  - Antibiotic resistance
KW  - Virulence genes
UR  - https://link.springer.com/article/10.1007/s12223-024-01237-1
N2  - Medical students are exposed to the hospital environment and patients during their studies, increasing the risk of exposure to virulent and antibiotic-resistant isolates of Staphylococcus aureus. The aim of the study is to determine the prevalence of Staphylococcus aureus among medical students who have varying levels of exposure to the hospital environment to provide valuable insights into the risk of colonization and transmission. Nasal swabs and fingerprints were obtained and cultured on a selective medium for staphylococci. The obtained isolates were confirmed as methicillin-sensitive S. aureus (MSSA) or methicillin-resistant (MRSA) using PCR. Antibiotic resistance, the presence of virulence genes including enterotoxin encoding genes, and spa typing were performed. Among pre-clinical students, MSSA was detected on the nose in 45.2% and on the fingerprints in 10.6% of the participants. Among clinical students, MSSA was detected on the nose in 42.0% and on the fingerprints in 25.4%. Only one MRSA isolate was obtained. Genes seg and sei were the most frequently detected in both student groups, with their presence in over 40% of isolates among clinical students. The eta and etb genes were mainly detected from the nose in both student groups. In pre-clinical students, S. aureus carrying eta gene occurred in 6.4% and etb in 8.5%. In clinical students, the occurrence was 5.1% for eta and 8.5% for etb. The tst gene was identified only in the nose and fingerprints of the clinical student group. The most frequently observed resistance was to clindamycin and erythromycin. In total 58 different spa types were identified. High rates of asymptomatic MSSA carriage were observed in both groups of medical students. Detected MSSA strains showed a high degree of genetic variability, with a number of them carrying the virulence and antibiotic resistance genes. Although students do not exhibit increased risk to their patient’s, increased hygiene is required in asymptomatic carriage personnel. The overall prevalence of MRSA was low, with a minimal risk of spread.
ER  -

BRODÍKOVÁ, Kristýna; Bohdana REZKOVÁ; Ivana KOLÁČKOVÁ a Renata KARPÍŠKOVÁ. Asymptomatic carriage and molecular characterization of Staphylococcus aureus in pre-clinical and clinical medical students. \textit{FOLIA MICROBIOLOGICA}. DORDRECHT: SPRINGER, 2025, roč.~70, č.~1, s.~241-248. ISSN~0015-5632. Dostupné z: https://doi.org/10.1007/s12223-024-01237-1.

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