An enzyme cascade biosensor based on multiwalled carbon
nanotube-RuO2 nanocomposite for selective amperometric
determination of lactose in milk samples

J 2024

An enzyme cascade biosensor based on multiwalled carbon nanotube-RuO2 nanocomposite for selective amperometric determination of lactose in milk samples

GAREHBAGHI, Sanam; Vojtech ADAM; Jan PŘIBYL; Lukas RICHTERA; Amir M ASHRAFI et. al.

Základní údaje

Originální název

An enzyme cascade biosensor based on multiwalled carbon nanotube-RuO2 nanocomposite for selective amperometric determination of lactose in milk samples

Autoři

GAREHBAGHI, Sanam; Vojtech ADAM; Jan PŘIBYL (203 Česká republika, domácí); Lukas RICHTERA a Amir M ASHRAFI

Vydání

Microchemical Journal, AMSTERDAM, Elsevier, 2024, 0026-265X

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10406 Analytical chemistry

Stát vydavatele

Nizozemské království

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 4.900 v roce 2023

Kód RIV

RIV/00216224:14740/24:00138500

Organizační jednotka

Středoevropský technologický institut

DOI

http://dx.doi.org/10.1016/j.microc.2024.111138

UT WoS

001272028000001

EID Scopus

2-s2.0-85198242094

Klíčová slova anglicky

Lactose determination; Enzyme cascade system; Electrochemical biosensor; Chronoamperometry; Semi skim milk

Štítky

CF NANO, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 3. 6. 2025 13:31, Mgr. Eva Dubská

Anotace

V originále

An enzyme-based electrochemical biosensor was fabricated for the sensitive determination of lactose. The utilized enzyme cascade system is composed of 8-galactosidase (8-Gal) and glucose oxidase (GOx). The ruthenium (IV) oxide (RuO2) presents in MWCNT-RuO2 nanocomposite immobilized on the glassy carbon electrode acts as an electrochemical mediator, resembling a second-generation enzyme biosensor. The functional mechanism of the biosensor was discussed, explaining the chemical oxidation of H2O2, the final product of the enzymatic reaction, by RuO2 and subsequent reoxidation of generated Ru to RuO2 at the electrode surface. This shifts the oxidation of H2O2 to a lower potential magnitude of +0.40 V, enhancing the biosensor's selectivity. The analytical figures of merit were verified for the developed lactose biosensor through repetitive measurements. The precision of the lactose biosensor was ensured with good reproducibility (RSD % = 2.68) and repeatability (RSD % = 4.12). The selectivity of the biosensor towards various saccharides and ionic species potentially present in milk samples was investigated, and no notable interference effect was detected. Moreover, the accuracy of the lactose biosensor was tested by analyzing spiked samples and a semi-skimmed milk (SS-milk) sample with certified lactose values. The limit of detection (LOD) and limit of quantification (LOQ) were calculated to be (0.036 mM) and (0.121 mM), respectively. Thanks to the short response time of the fabricated lactose biosensor, it was transferred to a screen-printed carbon electrode (SPCE) and successfully employed in flow injection analysis (FIA).

Návaznosti

EF18_046/0015974, projekt VaV

Název: Modernizace České infrastruktury pro integrativní strukturní biologii

90242, velká výzkumná infrastruktura

Název: CIISB III

Citovat

GAREHBAGHI, Sanam; Vojtech ADAM; Jan PŘIBYL; Lukas RICHTERA a Amir M ASHRAFI. An enzyme cascade biosensor based on multiwalled carbon nanotube-RuO2 nanocomposite for selective amperometric determination of lactose in milk samples. Microchemical Journal. AMSTERDAM: Elsevier, 2024, roč. 204, sep, s. 1-12. ISSN 0026-265X. Dostupné z: https://dx.doi.org/10.1016/j.microc.2024.111138.

@article{2468531,
   author = {Garehbaghi, Sanam and Adam, Vojtech and Přibyl, Jan and Richtera, Lukas and Ashrafi, Amir M},
   article_location = {AMSTERDAM},
   article_number = {sep},
   doi = {http://dx.doi.org/10.1016/j.microc.2024.111138},
   keywords = {Lactose determination; Enzyme cascade system; Electrochemical biosensor; Chronoamperometry; Semi skim milk},
   language = {eng},
   issn = {0026-265X},
   journal = {Microchemical Journal},
   title = {An enzyme cascade biosensor based on multiwalled carbon nanotube-RuO2 nanocomposite for selective amperometric determination of lactose in milk samples},
   url = {https://www.sciencedirect.com/science/article/pii/S0026265X24012505?via%3Dihub},
   volume = {204},
   year = {2024}
}

TY  - JOUR
ID  - 2468531
AU  - Garehbaghi, Sanam - Adam, Vojtech - Přibyl, Jan - Richtera, Lukas - Ashrafi, Amir M
PY  - 2024
TI  - An enzyme cascade biosensor based on multiwalled carbon nanotube-RuO2 nanocomposite for selective amperometric determination of lactose in milk samples
JF  - Microchemical Journal
VL  - 204
IS  - sep
SP  - 1-12
EP  - 1-12
PB  - Elsevier
SN  - 0026265X
KW  - Lactose determination
KW  - Enzyme cascade system
KW  - Electrochemical biosensor
KW  - Chronoamperometry
KW  - Semi skim milk
UR  - https://www.sciencedirect.com/science/article/pii/S0026265X24012505?via%3Dihub
N2  - An enzyme-based electrochemical biosensor was fabricated for the sensitive determination of lactose. The utilized enzyme cascade system is composed of 8-galactosidase (8-Gal) and glucose oxidase (GOx). The ruthenium (IV) oxide (RuO2) presents in MWCNT-RuO2 nanocomposite immobilized on the glassy carbon electrode acts as an electrochemical mediator, resembling a second-generation enzyme biosensor. The functional mechanism of the biosensor was discussed, explaining the chemical oxidation of H2O2, the final product of the enzymatic reaction, by RuO2 and subsequent reoxidation of generated Ru to RuO2 at the electrode surface. This shifts the oxidation of H2O2 to a lower potential magnitude of +0.40 V, enhancing the biosensor's selectivity. The analytical figures of merit were verified for the developed lactose biosensor through repetitive measurements. The precision of the lactose biosensor was ensured with good reproducibility (RSD % = 2.68) and repeatability (RSD % = 4.12). The selectivity of the biosensor towards various saccharides and ionic species potentially present in milk samples was investigated, and no notable interference effect was detected. Moreover, the accuracy of the lactose biosensor was tested by analyzing spiked samples and a semi-skimmed milk (SS-milk) sample with certified lactose values. The limit of detection (LOD) and limit of quantification (LOQ) were calculated to be (0.036 mM) and (0.121 mM), respectively. Thanks to the short response time of the fabricated lactose biosensor, it was transferred to a screen-printed carbon electrode (SPCE) and successfully employed in flow injection analysis (FIA).
ER  -

GAREHBAGHI, Sanam; Vojtech ADAM; Jan PŘIBYL; Lukas RICHTERA a Amir M ASHRAFI. An enzyme cascade biosensor based on multiwalled carbon nanotube-RuO2 nanocomposite for selective amperometric determination of lactose in milk samples. \textit{Microchemical Journal}. AMSTERDAM: Elsevier, 2024, roč.~204, sep, s.~1-12. ISSN~0026-265X. Dostupné z: https://dx.doi.org/10.1016/j.microc.2024.111138.

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