The importance of extracellular vesicles as potential
biomarkers of head and neck cancer

k 2024

The importance of extracellular vesicles as potential biomarkers of head and neck cancer

HÁNĚLOVÁ, Klára

Základní údaje

Originální název

The importance of extracellular vesicles as potential biomarkers of head and neck cancer

Autoři

HÁNĚLOVÁ, Klára

Vydání

ASEV-CzeSEV, A joint meeting of the Extracellular Vesicles Societies in Austria and Czech Republic, 2024, 2024

Další údaje

Jazyk

angličtina

Typ výsledku

Prezentace na konferencích

Utajení

není předmětem státního či obchodního tajemství

Označené pro přenos do RIV

Organizační jednotka

Lékařská fakulta

Změněno: 22. 1. 2025 14:22, Mgr. Klára Hánělová

Anotace

V originále

Head and neck squamous cell carcinoma (HNSCC) is, despite significant progress in cancer therapy, linked with poor prognosis due to the lack of early detection markers. Cancer-associated fibroblasts (CAFs) have recently gained a lot of attention, as they are involved in multiple aspects of cancer pathogenesis. Furthermore, CAFs often outgrow tumor cells and even resist treatment, thus they can serve as potential targets for anticancer therapies. These tumor-promoting effects are, besides others, mediated by extracellular vesicles (EVs). Thus, EV content can serve as a promising diagnostic and prognostic cancer biomarker. For the EV isolation, we used an affinity method based on phosphatidylserine (PS)-binding magnetic beads to separate PS-positive EVs (PS-EVs) from cell culture media of FaDu cell line (hypopharyngeal carcinoma) and CAFs derived from HNSCC patients. Since PS serves as an “eat me signal”, we presumed that PS-EVs would be uptaken by recipient cells. All EV samples were characterised by size (DLS), morphology (cryo-EM, TEM), typical protein markers (WB), and protein and RNA content. Our results showed that modulators of autophagy machinery change the protein content of EVs, which needs to be reminded when used in cancer therapy. Interestingly, cancer-derived EVs were highly enriched in mitochondrial proteins, especially when glutamine starvation was induced in cancer cells. The protein content of CAF-derived EVs seems to correspond with different stages of the disease, which points to the importance of CAFs and EVs as promising biomarkers in HNSCC.

Citovat

HÁNĚLOVÁ, Klára. The importance of extracellular vesicles as potential biomarkers of head and neck cancer. In ASEV-CzeSEV, A joint meeting of the Extracellular Vesicles Societies in Austria and Czech Republic, 2024. 2024.

@proceedings{2470179,
   author = {Hánělová, Klára},
   booktitle = {ASEV-CzeSEV, A joint meeting of the Extracellular Vesicles Societies in Austria and Czech Republic, 2024},
   language = {eng},
   title = {The importance of extracellular vesicles as potential biomarkers of head and neck cancer},
   year = {2024}
}

TY  - CONF
ID  - 2470179
AU  - Hánělová, Klára
PY  - 2024
TI  - The importance of extracellular vesicles as potential biomarkers of head and neck cancer
N2  - Head and neck squamous cell carcinoma (HNSCC) is, despite significant progress in cancer therapy, linked with poor prognosis due to the lack of early detection markers. Cancer-associated fibroblasts (CAFs) have recently gained a lot of attention, as they are involved in multiple aspects of cancer pathogenesis. Furthermore, CAFs often outgrow tumor cells and even resist treatment, thus they can serve as potential targets for anticancer therapies. These tumor-promoting effects are, besides others, mediated by extracellular vesicles (EVs). Thus, EV content can serve as a promising diagnostic and prognostic cancer biomarker. For the EV isolation, we used an affinity method based on phosphatidylserine (PS)-binding magnetic beads to separate PS-positive EVs (PS-EVs) from cell culture media of FaDu cell line (hypopharyngeal carcinoma) and CAFs derived from HNSCC patients. Since PS serves as an “eat me signal”, we presumed that PS-EVs would be uptaken by recipient cells. All EV samples were characterised by size (DLS), morphology (cryo-EM, TEM), typical protein markers (WB), and protein and RNA content. Our results showed that modulators of autophagy machinery change the protein content of EVs, which needs to be reminded when used in cancer therapy. Interestingly, cancer-derived EVs were highly enriched in mitochondrial proteins, especially when glutamine starvation was induced in cancer cells. The protein content of CAF-derived EVs seems to correspond with different stages of the disease, which points to the importance of CAFs and EVs as promising biomarkers in HNSCC.
ER  -

HÁNĚLOVÁ, Klára. The importance of extracellular vesicles as potential biomarkers of head and neck cancer. In \textit{ASEV-CzeSEV, A joint meeting of the Extracellular Vesicles Societies in Austria and Czech Republic, 2024}. 2024.

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