The Role of Phosphatidylserine-positive Extracellular Vesicles
in Intercellular Communication

k 2023

The Role of Phosphatidylserine-positive Extracellular Vesicles in Intercellular Communication

HÁNĚLOVÁ, Klára; Jan BALVAN; Martina RAUDENSKÁ; Květa PILAŘOVÁ; Monika KRATOCHVÍLOVÁ et al.

Základní údaje

Originální název

The Role of Phosphatidylserine-positive Extracellular Vesicles in Intercellular Communication

Autoři

HÁNĚLOVÁ, Klára; Jan BALVAN; Martina RAUDENSKÁ ; Květa PILAŘOVÁ; Monika KRATOCHVÍLOVÁ ; Jiří NAVRÁTIL ; Tomáš VIČAR; Mária BUGAJOVÁ ; Jaromír GUMULEC a Michal MASAŘÍK

Vydání

XXII. Setkání biochemiků a molekulárních biologů, 2023, 2023

Další údaje

Jazyk

angličtina

Typ výsledku

Prezentace na konferencích

Utajení

není předmětem státního či obchodního tajemství

Označené pro přenos do RIV

Organizační jednotka

Lékařská fakulta

Změněno: 17. 2. 2025 07:06, Mgr. Tereza Miškechová

Anotace

V originále

Extracellular vesicles (EVs) are a heterogenous population of membrane vesicles derived from almost all cell types, even pathologically altered. Since they reflect the composition of their parental cells and are found in all body fluids, EVs are gaining attention by many researchers worldwide. While originally thought to function mainly in the elimination of cellular waste, EVs purpose is much more complex. EVs are essential mediators of intercellular communication and delivery particles of molecular signals. They substantially participate in maintaining homeostasis of healthy tissues and in progression of malignant disease transformation. Phosphatidylserine (PS) is a component of the plasma membrane, which occurs on the inner side of this membrane. PS is an important cell cycle signalling molecule, especially in relation to apoptosis. The exposure of PS on the outer surface of the membrane marks the apoptotic cell death. Furthermore, viable tumor cells often expose this phospholipid on their surface. Many studies also suggest that tumor cells release high amounts of EVs exposing PS at the surface. Accordingly, PS-EVs derived from tumor cells may be critically involved in the communication of tumor cells with other cells within the tumor microenvironment (TME). In this work, we isolated PS-EVs from head and neck squamous cell carcinoma (HNSCC) and cancer-associated fibroblasts (CAFs), characterised them and analysed their protein composition. The most represented proteins were found to be involved in processes, such as cell adhesion, and respectively, cell migration, apoptosis, angiogenesis, or intracellular signal transduction. As a next step, we observed the effect of these PS-EVs on normal fibroblasts (HGF) and hypopharyngeal carcinoma (FaDu cell line). After the influence of PS-EVs derived from CAF 101, FaDu cells showed resistance to apoptosis, thus EVs could serve as fundamental signal particles with high impact on tumor progression.

Citovat

HÁNĚLOVÁ, Klára; Jan BALVAN; Martina RAUDENSKÁ; Květa PILAŘOVÁ; Monika KRATOCHVÍLOVÁ; Jiří NAVRÁTIL; Tomáš VIČAR; Mária BUGAJOVÁ; Jaromír GUMULEC a Michal MASAŘÍK. The Role of Phosphatidylserine-positive Extracellular Vesicles in Intercellular Communication. In XXII. Setkání biochemiků a molekulárních biologů, 2023. 2023.

@proceedings{2470180,
   author = {Hánělová, Klára and Balvan, Jan and Raudenská, Martina and Pilařová, Květa and Kratochvílová, Monika and Navrátil, Jiří and Vičar, Tomáš and Bugajová, Mária and Gumulec, Jaromír and Masařík, Michal},
   booktitle = {XXII. Setkání biochemiků a molekulárních biologů, 2023},
   language = {eng},
   title = {The Role of Phosphatidylserine-positive Extracellular Vesicles in Intercellular Communication},
   year = {2023}
}

TY  - CONF
ID  - 2470180
AU  - Hánělová, Klára - Balvan, Jan - Raudenská, Martina - Pilařová, Květa - Kratochvílová, Monika - Navrátil, Jiří - Vičar, Tomáš - Bugajová, Mária - Gumulec, Jaromír - Masařík, Michal
PY  - 2023
TI  - The Role of Phosphatidylserine-positive Extracellular Vesicles in Intercellular Communication
N2  - Extracellular vesicles (EVs) are a heterogenous population of membrane vesicles derived from almost all cell types, even pathologically altered. Since they reflect the composition of their parental cells and are found in all body fluids, EVs are gaining attention by many researchers worldwide. While originally thought to function mainly in the elimination of cellular waste, EVs purpose is much more complex. EVs are essential mediators of intercellular communication and delivery particles of molecular signals. They substantially participate in maintaining homeostasis of healthy tissues and in progression of malignant disease transformation. Phosphatidylserine (PS) is a component of the plasma membrane, which occurs on the inner side of this membrane. PS is an important cell cycle signalling molecule, especially in relation to apoptosis. The exposure of PS on the outer surface of the membrane marks the apoptotic cell death. Furthermore, viable tumor cells often expose this phospholipid on their surface. Many studies also suggest that tumor cells release high amounts of EVs exposing PS at the surface. Accordingly, PS-EVs derived from tumor cells may be critically involved in the communication of tumor cells with other cells within the tumor microenvironment (TME). In this work, we isolated PS-EVs from head and neck squamous cell carcinoma (HNSCC) and cancer-associated fibroblasts (CAFs), characterised them and analysed their protein composition. The most represented proteins were found to be involved in processes, such as cell adhesion, and respectively, cell migration, apoptosis, angiogenesis, or intracellular signal transduction. As a next step, we observed the effect of these PS-EVs on normal fibroblasts (HGF) and hypopharyngeal carcinoma (FaDu cell line). After the influence of PS-EVs derived from CAF 101, FaDu cells showed resistance to apoptosis, thus EVs could serve as fundamental signal particles with high impact on tumor progression.
ER  -

HÁNĚLOVÁ, Klára; Jan BALVAN; Martina RAUDENSKÁ; Květa PILAŘOVÁ; Monika KRATOCHVÍLOVÁ; Jiří NAVRÁTIL; Tomáš VIČAR; Mária BUGAJOVÁ; Jaromír GUMULEC a Michal MASAŘÍK. The Role of Phosphatidylserine-positive Extracellular Vesicles in Intercellular Communication. In \textit{XXII. Setkání biochemiků a molekulárních biologů, 2023}. 2023.

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