Preparation and Complex Characterisation of Stabilised Gold
Nanoparticles: Biodistribution and Application for High
Resolution In Vivo Imaging

J 2024

Preparation and Complex Characterisation of Stabilised Gold Nanoparticles: Biodistribution and Application for High Resolution In Vivo Imaging

TURANEK, Jaroslav; Pavlina TURANEK KNOTIGOVA; Pavel KULICH; Radim SKOUPY; Kamila HRUBANOVA et al.

Základní údaje

Originální název

Preparation and Complex Characterisation of Stabilised Gold Nanoparticles: Biodistribution and Application for High Resolution In Vivo Imaging

Autoři

TURANEK, Jaroslav; Pavlina TURANEK KNOTIGOVA; Pavel KULICH; Radim SKOUPY; Kamila HRUBANOVA; Naděžda VAŠKOVICOVÁ; Ladislav FEKETE; Antonin KANA; Robert MIKULÍK a Milan RASKA

Vydání

Pharmaceuticals, BASEL, MDPI, 2024, 1424-8247

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30104 Pharmacology and pharmacy

Stát vydavatele

Švýcarsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 4.800

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14110/24:00138596

Organizační jednotka

Lékařská fakulta

Klíčová slova anglicky

gold nanoparticles; in vivo imaging; microcomputer tomography; biodistribution; nanotoxicology

Štítky

14110517, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 23. 1. 2025 11:45, Mgr. Tereza Miškechová

Anotace

V originále

The Turkevich method was optimized to prepare gold nanoparticles (AuNP) stabilized by polyethyleneglycol (PEG) for mu CT. Using various independent modalities, we thoroughly characterized the optimized PEG-AuNPs. Here, we show that PEG-AuNPs are retained in the blood and provide a high contrast in the high-resolution mu CT imaging of blood vessels and inner organs. The biodistribution is characterized by prolonged circulation in the blood and accumulation in the liver, spleen and skin. The accumulation of AuNP in the skin resulted in the blue discoloration of eyes and the whole skin. In vitro experiments using a leukemic monocyte THP-1 cell line model expressing high levels of NLRP3 demonstrated that the NLRP3inflammasome was not activated by PEG AuNP. Over 9 months, the mice were scanned by mu CT and were in good health. Scans in mice using PEG-stabilized AuNPs in this study were sharper, with a higher contrast, when compared to a commercial contrasting agent at the same dose. The PEG-AuNPs were morphologically and chemically stable for at least two years when stored in the refrigerator.

Návaznosti

EH22_008/0004596, projekt VaV

Název: Senzory a detektory pro informační společnost budoucnosti

NU21-08-00510, projekt VaV

Název: Biokompatibilní nanočástice jako cílené systémy pro dodávání léčiv a teranostik pro léčbu cévní mozkové příhody

Investor: Ministerstvo zdravotnictví ČR, Biokompatibilní nanočástice jako cílené systémy pro dodávání léčiv a teranostik pro léčbu cévní mozkové příhody

Citovat

TURANEK, Jaroslav; Pavlina TURANEK KNOTIGOVA; Pavel KULICH; Radim SKOUPY; Kamila HRUBANOVA; Naděžda VAŠKOVICOVÁ; Ladislav FEKETE; Antonin KANA; Robert MIKULÍK a Milan RASKA. Preparation and Complex Characterisation of Stabilised Gold Nanoparticles: Biodistribution and Application for High Resolution In Vivo Imaging. Pharmaceuticals. BASEL: MDPI, 2024, roč. 17, č. 11, s. 1-16. ISSN 1424-8247. Dostupné z: https://doi.org/10.3390/ph17111479.

@article{2470317,
   author = {Turanek, Jaroslav and Turanek Knotigova, Pavlina and Kulich, Pavel and Skoupy, Radim and Hrubanova, Kamila and Vaškovicová, Naděžda and Fekete, Ladislav and Kana, Antonin and Mikulík, Robert and Raska, Milan},
   article_location = {BASEL},
   article_number = {11},
   doi = {https://doi.org/10.3390/ph17111479},
   keywords = {gold nanoparticles; in vivo imaging; microcomputer tomography; biodistribution; nanotoxicology},
   language = {eng},
   issn = {1424-8247},
   journal = {Pharmaceuticals},
   title = {Preparation and Complex Characterisation of Stabilised Gold Nanoparticles: Biodistribution and Application for High Resolution In Vivo Imaging},
   url = {https://www.mdpi.com/1424-8247/17/11/1479},
   volume = {17},
   year = {2024}
}

TY  - JOUR
ID  - 2470317
AU  - Turanek, Jaroslav - Turanek Knotigova, Pavlina - Kulich, Pavel - Skoupy, Radim - Hrubanova, Kamila - Vaškovicová, Naděžda - Fekete, Ladislav - Kana, Antonin - Mikulík, Robert - Raska, Milan
PY  - 2024
TI  - Preparation and Complex Characterisation of Stabilised Gold Nanoparticles: Biodistribution and Application for High Resolution In Vivo Imaging
JF  - Pharmaceuticals
VL  - 17
IS  - 11
SP  - 1-16
EP  - 1-16
PB  - MDPI
SN  - 14248247
KW  - gold nanoparticles
KW  - in vivo imaging
KW  - microcomputer tomography
KW  - biodistribution
KW  - nanotoxicology
UR  - https://www.mdpi.com/1424-8247/17/11/1479
N2  - The Turkevich method was optimized to prepare gold nanoparticles (AuNP) stabilized by polyethyleneglycol (PEG) for mu CT. Using various independent modalities, we thoroughly characterized the optimized PEG-AuNPs. Here, we show that PEG-AuNPs are retained in the blood and provide a high contrast in the high-resolution mu CT imaging of blood vessels and inner organs. The biodistribution is characterized by prolonged circulation in the blood and accumulation in the liver, spleen and skin. The accumulation of AuNP in the skin resulted in the blue discoloration of eyes and the whole skin. In vitro experiments using a leukemic monocyte THP-1 cell line model expressing high levels of NLRP3 demonstrated that the NLRP3inflammasome was not activated by PEG AuNP. Over 9 months, the mice were scanned by mu CT and were in good health. Scans in mice using PEG-stabilized AuNPs in this study were sharper, with a higher contrast, when compared to a commercial contrasting agent at the same dose. The PEG-AuNPs were morphologically and chemically stable for at least two years when stored in the refrigerator.
ER  -

TURANEK, Jaroslav; Pavlina TURANEK KNOTIGOVA; Pavel KULICH; Radim SKOUPY; Kamila HRUBANOVA; Naděžda VAŠKOVICOVÁ; Ladislav FEKETE; Antonin KANA; Robert MIKULÍK a Milan RASKA. Preparation and Complex Characterisation of Stabilised Gold Nanoparticles: Biodistribution and Application for High Resolution In Vivo Imaging. \textit{Pharmaceuticals}. BASEL: MDPI, 2024, roč.~17, č.~11, s.~1-16. ISSN~1424-8247. Dostupné z: https://doi.org/10.3390/ph17111479.

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