Fertilization by short-term stored sperm alters DNA methylation
patterns at single-base resolution in common carp Cyprinus
carpio embryos

J 2024

Fertilization by short-term stored sperm alters DNA methylation patterns at single-base resolution in common carp Cyprinus carpio embryos

CHENG, Yu; Songpei ZHANG; Rigolin NAYAK; Pavlina VECHTOVA; Fabian SCHUMACHER et al.

Základní údaje

Originální název

Fertilization by short-term stored sperm alters DNA methylation patterns at single-base resolution in common carp Cyprinus carpio embryos

Autoři

Vydání

REVIEWS IN FISH BIOLOGY AND FISHERIES, NETHERLANDS, SPRINGER, 2024, 0960-3166

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10617 Marine biology, freshwater biology, limnology

Stát vydavatele

Nizozemské království

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 4.600

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14740/24:00138651

Organizační jednotka

Středoevropský technologický institut

Klíčová slova anglicky

Sperm quality; WGBS; DNA damage; Embryo development; Sperm aging

Štítky

rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 9. 6. 2025 15:27, Mgr. Eva Dubská

Anotace

V originále

Sperm after short-term storage in vitro is widely used for artificial fertilization in aquaculture. It has been shown that short-term storage affects sperm motility characteristics, resulting in diminished fertility. However, the detrimental effects of short-term sperm storage on embryos development have remained unexplored in single-base methylome resolution. The main aim of the present study was to investigate DNA methylation in the offspring of common carp (Cyprinus carpio) derived from short-term stored sperm. Sperm were stored in artificial seminal plasma on ice (0-2 degrees C) for 0, 3 and 6 days in vitro, fertilization was performed using oocytes from a single female, and embryos were collected at the mid-blastula stage. In the DNA methylation study, DNA from both sperm and embryos was extracted and analysed using liquid chromatography with tandem mass spectrometry (LC-MS/MS). Concurrently, DNA methylation levels of embryos in single base were evaluated through whole genome bisulfite sequencing (WGBS). Sperm storage showed negative effects on sperm motility, viability, and DNA integrity, but had no effect on global DNA methylation of spermatozoa and resulting embryos. Results from the WGBS showed that methylation of 3313 differentially methylated regions (DMRs)-target genes was affected in the embryos fertilized with the 6-day-stored sperm, and the identified DMRs were mainly involved in cell adhesion, calcium, mitogen-activated protein kinase and adrenergic signalling, melanogenesis, metabolism and RNA transport. Such results suggest that prolongation of storage time may have certain impacts on embryonic development. These initial results provide valuable information for future consideration of the DNA methylome in embryos generated from short-term stored sperm, which are used for genetic management of broodstock in aquaculture.

Citovat

CHENG, Yu; Songpei ZHANG; Rigolin NAYAK; Pavlina VECHTOVA; Fabian SCHUMACHER; Pavla LINHARTOVÁ; Ievgeniia GAZO; Zuzana LINHARTOVA; Swapnil Gorakh WAGHMARE; Burkhard KLEUSER; Abhipsha DEY; Vladimira RODINOVA; Marek RODINA; Jan STERBA; Sayyed Mohammad Hadi ALAVI; Catherine LABBE a Otomar LINHART. Fertilization by short-term stored sperm alters DNA methylation patterns at single-base resolution in common carp Cyprinus carpio embryos. REVIEWS IN FISH BIOLOGY AND FISHERIES. NETHERLANDS: SPRINGER, 2024, roč. 34, č. 3, s. 1167-1187. ISSN 0960-3166. Dostupné z: https://doi.org/10.1007/s11160-024-09866-y.

@article{2471624,
   author = {Cheng, Yu and Zhang, Songpei and Nayak, Rigolin and Vechtova, Pavlina and Schumacher, Fabian and Linhartová, Pavla and Gazo, Ievgeniia and Linhartova, Zuzana and Waghmare, Swapnil Gorakh and Kleuser, Burkhard and Dey, Abhipsha and Rodinova, Vladimira and Rodina, Marek and Sterba, Jan and Alavi, Sayyed Mohammad Hadi and Labbe, Catherine and Linhart, Otomar},
   article_location = {NETHERLANDS},
   article_number = {3},
   doi = {https://doi.org/10.1007/s11160-024-09866-y},
   keywords = {Sperm quality; WGBS; DNA damage; Embryo development; Sperm aging},
   language = {eng},
   issn = {0960-3166},
   journal = {REVIEWS IN FISH BIOLOGY AND FISHERIES},
   title = {Fertilization by short-term stored sperm alters DNA methylation patterns at single-base resolution in common carp Cyprinus carpio embryos},
   url = {https://link.springer.com/article/10.1007/s11160-024-09866-y},
   volume = {34},
   year = {2024}
}

TY  - JOUR
ID  - 2471624
AU  - Cheng, Yu - Zhang, Songpei - Nayak, Rigolin - Vechtova, Pavlina - Schumacher, Fabian - Linhartová, Pavla - Gazo, Ievgeniia - Linhartova, Zuzana - Waghmare, Swapnil Gorakh - Kleuser, Burkhard - Dey, Abhipsha - Rodinova, Vladimira - Rodina, Marek - Sterba, Jan - Alavi, Sayyed Mohammad Hadi - Labbe, Catherine - Linhart, Otomar
PY  - 2024
TI  - Fertilization by short-term stored sperm alters DNA methylation patterns at single-base resolution in common carp Cyprinus carpio embryos
JF  - REVIEWS IN FISH BIOLOGY AND FISHERIES
VL  - 34
IS  - 3
SP  - 1167-1187
EP  - 1167-1187
PB  - SPRINGER
SN  - 09603166
KW  - Sperm quality
KW  - WGBS
KW  - DNA damage
KW  - Embryo development
KW  - Sperm aging
UR  - https://link.springer.com/article/10.1007/s11160-024-09866-y
N2  - Sperm after short-term storage in vitro is widely used for artificial fertilization in aquaculture. It has been shown that short-term storage affects sperm motility characteristics, resulting in diminished fertility. However, the detrimental effects of short-term sperm storage on embryos development have remained unexplored in single-base methylome resolution. The main aim of the present study was to investigate DNA methylation in the offspring of common carp (Cyprinus carpio) derived from short-term stored sperm. Sperm were stored in artificial seminal plasma on ice (0-2 degrees C) for 0, 3 and 6 days in vitro, fertilization was performed using oocytes from a single female, and embryos were collected at the mid-blastula stage. In the DNA methylation study, DNA from both sperm and embryos was extracted and analysed using liquid chromatography with tandem mass spectrometry (LC-MS/MS). Concurrently, DNA methylation levels of embryos in single base were evaluated through whole genome bisulfite sequencing (WGBS). Sperm storage showed negative effects on sperm motility, viability, and DNA integrity, but had no effect on global DNA methylation of spermatozoa and resulting embryos. Results from the WGBS showed that methylation of 3313 differentially methylated regions (DMRs)-target genes was affected in the embryos fertilized with the 6-day-stored sperm, and the identified DMRs were mainly involved in cell adhesion, calcium, mitogen-activated protein kinase and adrenergic signalling, melanogenesis, metabolism and RNA transport. Such results suggest that prolongation of storage time may have certain impacts on embryonic development. These initial results provide valuable information for future consideration of the DNA methylome in embryos generated from short-term stored sperm, which are used for genetic management of broodstock in aquaculture.
ER  -

CHENG, Yu; Songpei ZHANG; Rigolin NAYAK; Pavlina VECHTOVA; Fabian SCHUMACHER; Pavla LINHARTOVÁ; Ievgeniia GAZO; Zuzana LINHARTOVA; Swapnil Gorakh WAGHMARE; Burkhard KLEUSER; Abhipsha DEY; Vladimira RODINOVA; Marek RODINA; Jan STERBA; Sayyed Mohammad Hadi ALAVI; Catherine LABBE a Otomar LINHART. Fertilization by short-term stored sperm alters DNA methylation patterns at single-base resolution in common carp Cyprinus carpio embryos. \textit{REVIEWS IN FISH BIOLOGY AND FISHERIES}. NETHERLANDS: SPRINGER, 2024, roč.~34, č.~3, s.~1167-1187. ISSN~0960-3166. Dostupné z: https://doi.org/10.1007/s11160-024-09866-y.

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