2024
Molecular and serological detection of Borrelia burgdorferi sensu lato in small free-living mammals
KOZLÍKOVÁ, Edita; Eva BÁRTOVÁ a Alena ŽÁKOVSKÁZákladní údaje
Originální název
Molecular and serological detection of Borrelia burgdorferi sensu lato in small free-living mammals
Název česky
Molekulární a sérologická detekce Borrelia burgdorferi sensu lato u drobných volně žijících savců
Název anglicky
Molecular and serological detection of Borrelia burgdorferi sensu lato in small free-living mammals
Autoři
KOZLÍKOVÁ, Edita; Eva BÁRTOVÁ a Alena ŽÁKOVSKÁ
Vydání
Brno, XXVI. KONFERENCE MLADÝCH VĚDECKÝCH PRACOVNÍKŮ S MEZINÁRODNÍ ÚČASTÍ, od s. 85-87, 3 s. 2024
Nakladatel
VETERINÁRNÍ UNIVERZITA BRNO
Další údaje
Jazyk
čeština
Typ výsledku
Stať ve sborníku
Obor
30303 Infectious Diseases
Stát vydavatele
Česká republika
Utajení
není předmětem státního či obchodního tajemství
Forma vydání
elektronická verze "online"
Odkazy
Označené pro přenos do RIV
Ano
Kód RIV
RIV/00216224:14310/24:00138668
Organizační jednotka
Přírodovědecká fakulta
ISBN
978-80-7305-957-6
Klíčová slova česky
B. burgdorferi s.l.; small mammals; Apodemus sylvaticus; PCR
Klíčová slova anglicky
B. burgdorferi s.l.; small mammals; Apodemus sylvaticus; PCR
Příznaky
Recenzováno
Změněno: 20. 2. 2025 13:41, doc. RNDr. Alena Žákovská, Ph.D.
V originále
Free living mammals serve as reservoir animals for the spirochetes Borrelia burgdorferi s.l. from which the infection can be transmitted through blood sucking insects to other hosts, in which a disease called Lyme disease can occur. The presence of B. burgdorferi s.l. was determined in 170 small mammals caught in the wild according to the approved experimental project. During the autopsy, tissue samples (heart rinses and kidneys) were taken for antibody detection by the ELISA method and for direct detection of B. burgdorferi s.l. by PCR. The species of the animal was determined by using a morphological key, if there was doubt about the distinction between Apodemus flavicollis and Apodemus sylvaticus, AP PCR was used. The prevalence of antibodies against B. burgdorferi s.l. was 10.5% (18/170) for IgM and 10% (17/170) for IgG without statistically significant difference (p>0.05) between the species, age, sex of animals and year season. The presence of B. burgdorferi s.l. was detected by PCR in 14.7% (25/170) of animals. No statistical significance was found in species, age and sex. The positivity statistically significantly differed in the seasons (p=0.021) with the highest positivity in sp ring and lowest positivity in autumn. We expected both antibody and Bbsl prevalence results around the landfill to be higher than in other areas due to the high presence of rodents in a small area, but the results are consistent with findings from other areas, so landfills are not more dangerous and there is no higher risk of Bbsl spreading. than in, for example, a forest park.
Anglicky
Free living mammals serve as reservoir animals for the spirochetes Borrelia burgdorferi s.l. from which the infection can be transmitted through blood sucking insects to other hosts, in which a disease called Lyme disease can occur. The presence of B. burgdorferi s.l. was determined in 170 small mammals caught in the wild according to the approved experimental project. During the autopsy, tissue samples (heart rinses and kidneys) were taken for antibody detection by the ELISA method and for direct detection of B. burgdorferi s.l. by PCR. The species of the animal was determined by using a morphological key, if there was doubt about the distinction between Apodemus flavicollis and Apodemus sylvat icus, AP PCR was used. The prevalence of antibodies against B. burgdorferi s.l. was 10.5% (18/170) for IgM and 10% (17/170) for IgG without statistically significant difference (p>0.05) between the species, age, sex of animals and year season. The presence of B. burgdorferi s.l. was detected by PCR in 14.7% (25/170) of animals. No statistical significance was found in species, age and sex (p>0.05). The positivity statistically significantly differed in the seasons (p=0.021) with the highest positivity in sp ring and lowest positivity in autumn. We expected both antibody and Bbsl prevalence results around the landfill to be higher than in other areas due to the high presence of rodents in a small area, but the results are consistent with findings from other areas, so landfills are not more dangerous and there is no higher risk of Bbsl spreading. than in, for example, a forest park.
Návaznosti
| MUNI/A/1258/2022, interní kód MU |
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