The Blood-Cerebrospinal Fluid Barrier as a Potential Entry Site
for the SARS-CoV-2 Virus

J 2025

The Blood-Cerebrospinal Fluid Barrier as a Potential Entry Site for the SARS-CoV-2 Virus

SOLÁR, Peter; Omar ŠERÝ; Tomáš VOJTÍŠEK; Jan KRAJSA; Michal SRNÍK et. al.

Základní údaje

Originální název

The Blood-Cerebrospinal Fluid Barrier as a Potential Entry Site for the SARS-CoV-2 Virus

Autoři

SOLÁR, Peter; Omar ŠERÝ; Tomáš VOJTÍŠEK; Jan KRAJSA; Michal SRNÍK; Radka DZIEDZINSKÁ; Petr KRÁLÍK; Markéta KESSLER; Petr DUBOVÝ; Andrea JOUKAL; Vladimír Josef BALCAR a Marek JOUKAL

Vydání

Journal of Medical Virology, Hoboken, Wiley, 2025, 0146-6615

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10607 Virology

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 4.600 v roce 2024

Organizační jednotka

Lékařská fakulta

DOI

https://doi.org/10.1002/jmv.70184

UT WoS

001400677300001

EID Scopus

2-s2.0-85215678630

Klíčová slova anglicky

blood-cerebrospinal fluid barrier; choroid plexus; COVID-19; neuroinvasion; SARS-CoV-2

Štítky

14110111, 14110514, 14313050, podil, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 28. 1. 2025 14:58, Mgr. Tereza Miškechová

Anotace

V originále

Severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) is an RNA virus responsible for coronavirus disease 2019 (COVID-19). While SARS-CoV-2 primarily targets the lungs and airways, it can also infect other organs, including the central nervous system (CNS). The aim of this study was to investigate whether the choroid plexus could serve as a potential entry site for SARS-CoV-2 into the brain. Tissue samples from 24 deceased COVID-19-positive individuals were analyzed. Reverse transcription real-time PCR (RT-qPCR) was performed on selected brain regions, including the choroid plexus, to detect SARS-CoV-2 viral RNA. Additionally, immunofluorescence staining and confocal microscopy were used to detect and localize two characteristic proteins of SARS-CoV-2: the spike protein S1 and the nucleocapsid protein. RT-qPCR analysis confirmed the presence of SARS-CoV-2 viral RNA in the choroid plexus. Immunohistochemical staining revealed viral particles localized in the epithelial cells of the choroid plexus, with the spike protein S1 detected in the late endosomes. Our findings suggest that the blood-cerebrospinal fluid (B-CSF) barrier in the choroid plexus serves as a route of entry for SARS-CoV-2 into the CNS. This study contributes to the understanding of the mechanisms underlying CNS involvement in COVID-19 and highlights the importance of further research to explore potential therapeutic strategies targeting this entry pathway.

Návaznosti

MUNI/A/1563/2023, interní kód MU

Název: Funkční morfologie: od molekulární biologie ke klinické anatomii 3

Investor: Masarykova univerzita, Funkční morfologie: od molekulární biologie ke klinické anatomii 3

Citovat

SOLÁR, Peter; Omar ŠERÝ; Tomáš VOJTÍŠEK; Jan KRAJSA; Michal SRNÍK; Radka DZIEDZINSKÁ; Petr KRÁLÍK; Markéta KESSLER; Petr DUBOVÝ; Andrea JOUKAL; Vladimír Josef BALCAR a Marek JOUKAL. The Blood-Cerebrospinal Fluid Barrier as a Potential Entry Site for the SARS-CoV-2 Virus. Journal of Medical Virology. Hoboken: Wiley, 2025, roč. 97, č. 1, s. 1-12. ISSN 0146-6615. Dostupné z: https://doi.org/10.1002/jmv.70184.

@article{2472000,
   author = {Solár, Peter and Šerý, Omar and Vojtíšek, Tomáš and Krajsa, Jan and Srník, Michal and Dziedzinská, Radka and Králík, Petr and Kessler, Markéta and Dubový, Petr and Joukal, Andrea and Balcar, Vladimír Josef and Joukal, Marek},
   article_location = {Hoboken},
   article_number = {1},
   doi = {https://doi.org/10.1002/jmv.70184},
   keywords = {blood-cerebrospinal fluid barrier; choroid plexus; COVID-19; neuroinvasion; SARS-CoV-2},
   language = {eng},
   issn = {0146-6615},
   journal = {Journal of Medical Virology},
   title = {The Blood-Cerebrospinal Fluid Barrier as a Potential Entry Site for the SARS-CoV-2 Virus},
   url = {https://onlinelibrary.wiley.com/doi/10.1002/jmv.70184},
   volume = {97},
   year = {2025}
}

TY  - JOUR
ID  - 2472000
AU  - Solár, Peter - Šerý, Omar - Vojtíšek, Tomáš - Krajsa, Jan - Srník, Michal - Dziedzinská, Radka - Králík, Petr - Kessler, Markéta - Dubový, Petr - Joukal, Andrea - Balcar, Vladimír Josef - Joukal, Marek
PY  - 2025
TI  - The Blood-Cerebrospinal Fluid Barrier as a Potential Entry Site for the SARS-CoV-2 Virus
JF  - Journal of Medical Virology
VL  - 97
IS  - 1
SP  - 1-12
EP  - 1-12
PB  - Wiley
SN  - 01466615
KW  - blood-cerebrospinal fluid barrier
KW  - choroid plexus
KW  - COVID-19
KW  - neuroinvasion
KW  - SARS-CoV-2
UR  - https://onlinelibrary.wiley.com/doi/10.1002/jmv.70184
N2  - Severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) is an RNA virus responsible for coronavirus disease 2019 (COVID-19). While SARS-CoV-2 primarily targets the lungs and airways, it can also infect other organs, including the central nervous system (CNS). The aim of this study was to investigate whether the choroid plexus could serve as a potential entry site for SARS-CoV-2 into the brain. Tissue samples from 24 deceased COVID-19-positive individuals were analyzed. Reverse transcription real-time PCR (RT-qPCR) was performed on selected brain regions, including the choroid plexus, to detect SARS-CoV-2 viral RNA. Additionally, immunofluorescence staining and confocal microscopy were used to detect and localize two characteristic proteins of SARS-CoV-2: the spike protein S1 and the nucleocapsid protein. RT-qPCR analysis confirmed the presence of SARS-CoV-2 viral RNA in the choroid plexus. Immunohistochemical staining revealed viral particles localized in the epithelial cells of the choroid plexus, with the spike protein S1 detected in the late endosomes. Our findings suggest that the blood-cerebrospinal fluid (B-CSF) barrier in the choroid plexus serves as a route of entry for SARS-CoV-2 into the CNS. This study contributes to the understanding of the mechanisms underlying CNS involvement in COVID-19 and highlights the importance of further research to explore potential therapeutic strategies targeting this entry pathway.
ER  -

SOLÁR, Peter; Omar ŠERÝ; Tomáš VOJTÍŠEK; Jan KRAJSA; Michal SRNÍK; Radka DZIEDZINSKÁ; Petr KRÁLÍK; Markéta KESSLER; Petr DUBOVÝ; Andrea JOUKAL; Vladimír Josef BALCAR a Marek JOUKAL. The Blood-Cerebrospinal Fluid Barrier as a Potential Entry Site for the SARS-CoV-2 Virus. \textit{Journal of Medical Virology}. Hoboken: Wiley, 2025, roč.~97, č.~1, s.~1-12. ISSN~0146-6615. Dostupné z: https://doi.org/10.1002/jmv.70184.

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