Tick-borne encephalitis virus modulates sphingolipid and
phospholipid metabolism in infected human neuronal cells

J 2024

Tick-borne encephalitis virus modulates sphingolipid and phospholipid metabolism in infected human neuronal cells

ŠIMEČKOVÁ, Pavlína; Josef SLAVÍK; Andrea FOŘTOVÁ; Ivana HUVAROVÁ; Lucie KRÁLIKOVÁ et al.

Základní údaje

Originální název

Tick-borne encephalitis virus modulates sphingolipid and phospholipid metabolism in infected human neuronal cells

Autoři

ŠIMEČKOVÁ, Pavlína; Josef SLAVÍK; Andrea FOŘTOVÁ; Ivana HUVAROVÁ; Lucie KRÁLIKOVÁ; Michal STEFANIK; Pavel SVOBODA; Daniel RŮŽEK a Miroslav MACHALA

Vydání

Microbes and Infection, Elsevier, 2024, 1286-4579

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10607 Virology

Stát vydavatele

Nizozemské království

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 2.700

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/24:00139725

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

Tick -borne encephalitis virus; Human neuronal cells; Sphingolipids; Targeted lipidomics; 4-HPR; Fenretinide

Štítky

14314010, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 30. 1. 2025 11:41, Mgr. Marie Novosadová Šípková, DiS.

Anotace

V originále

The life cycle of enveloped viruses is closely linked to host-cell lipids. However, changes in lipid metabolism during infections with the tick-borne encephalitis virus (TBEV) have not been described. TBEV is a medically important orthoflavivirus, which is endemic to many parts of Europe and Asia. In the present study, we performed targeted lipidomics with HPLC-MS/MS to evaluate changes in phospholipid and sphingolipid concentrations in TBEV-infected human neuronal SK-N-SH cells. TBEV infections significantly increased phosphatidylcholine, phosphatidylinositol, and phosphatidylserine levels within 48 h post-infection (hpi). Sphingolipids were slightly increased in dihydroceramides within 24 hpi. Later, at 48 hpi, the contents of sphinganine, dihydroceramides, ceramides, glucosylceramides, and ganglioside GD3 were elevated. On the other hand, sphingosine-1-phosphate content was slightly reduced in TBEVinfected cells. Changes in sphingolipid concentrations were accompanied by suppressed expression of a majority of the genes linked to sphingolipid and glycosphingolipid metabolism. Furthermore, we found that a pharmacological inhibitor of sphingolipid synthesis, fenretinide (4-HPR), inhibited TBEV infections in SK-N-SH cells. Taken together, our results suggested that both structural and signaling functions of lipids could be affected during TBEV infections. These changes might be connected to virus propagation and/or host-cell defense. (c) 2024 The Authors. Published by Elsevier Masson SAS on behalf of Institut Pasteur. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

Návaznosti

GA23-07160S, projekt VaV

Název: Molekulární patogeneze alimentární infekce virem klíšťové encefalitidy.

Investor: Grantová agentura ČR, Molekulární patogeneze alimentární infekce virem klíšťové encefalitidy.

Citovat

ŠIMEČKOVÁ, Pavlína; Josef SLAVÍK; Andrea FOŘTOVÁ; Ivana HUVAROVÁ; Lucie KRÁLIKOVÁ; Michal STEFANIK; Pavel SVOBODA; Daniel RŮŽEK a Miroslav MACHALA. Tick-borne encephalitis virus modulates sphingolipid and phospholipid metabolism in infected human neuronal cells. Microbes and Infection. Elsevier, 2024, roč. 26, č. 4, s. 1-11. ISSN 1286-4579. Dostupné z: https://doi.org/10.1016/j.micinf.2024.105303.

@article{2472604,
   author = {Šimečková, Pavlína and Slavík, Josef and Fořtová, Andrea and Huvarová, Ivana and Králiková, Lucie and Stefanik, Michal and Svoboda, Pavel and Růžek, Daniel and Machala, Miroslav},
   article_number = {4},
   doi = {https://doi.org/10.1016/j.micinf.2024.105303},
   keywords = {Tick -borne encephalitis virus; Human neuronal cells; Sphingolipids; Targeted lipidomics; 4-HPR; Fenretinide},
   language = {eng},
   issn = {1286-4579},
   journal = {Microbes and Infection},
   title = {Tick-borne encephalitis virus modulates sphingolipid and phospholipid metabolism in infected human neuronal cells},
   url = {https://www.sciencedirect.com/science/article/pii/S1286457924000236},
   volume = {26},
   year = {2024}
}

TY  - JOUR
ID  - 2472604
AU  - Šimečková, Pavlína - Slavík, Josef - Fořtová, Andrea - Huvarová, Ivana - Králiková, Lucie - Stefanik, Michal - Svoboda, Pavel - Růžek, Daniel - Machala, Miroslav
PY  - 2024
TI  - Tick-borne encephalitis virus modulates sphingolipid and phospholipid metabolism in infected human neuronal cells
JF  - Microbes and Infection
VL  - 26
IS  - 4
SP  - 1-11
EP  - 1-11
PB  - Elsevier
SN  - 12864579
KW  - Tick -borne encephalitis virus
KW  - Human neuronal cells
KW  - Sphingolipids
KW  - Targeted lipidomics
KW  - 4-HPR
KW  - Fenretinide
UR  - https://www.sciencedirect.com/science/article/pii/S1286457924000236
N2  - The life cycle of enveloped viruses is closely linked to host-cell lipids. However, changes in lipid metabolism during infections with the tick-borne encephalitis virus (TBEV) have not been described. TBEV is a medically important orthoflavivirus, which is endemic to many parts of Europe and Asia. In the present study, we performed targeted lipidomics with HPLC-MS/MS to evaluate changes in phospholipid and sphingolipid concentrations in TBEV-infected human neuronal SK-N-SH cells. TBEV infections significantly increased phosphatidylcholine, phosphatidylinositol, and phosphatidylserine levels within 48 h post-infection (hpi). Sphingolipids were slightly increased in dihydroceramides within 24 hpi. Later, at 48 hpi, the contents of sphinganine, dihydroceramides, ceramides, glucosylceramides, and ganglioside GD3 were elevated. On the other hand, sphingosine-1-phosphate content was slightly reduced in TBEVinfected cells. Changes in sphingolipid concentrations were accompanied by suppressed expression of a majority of the genes linked to sphingolipid and glycosphingolipid metabolism. Furthermore, we found that a pharmacological inhibitor of sphingolipid synthesis, fenretinide (4-HPR), inhibited TBEV infections in SK-N-SH cells. Taken together, our results suggested that both structural and signaling functions of lipids could be affected during TBEV infections. These changes might be connected to virus propagation and/or host-cell defense. (c) 2024 The Authors. Published by Elsevier Masson SAS on behalf of Institut Pasteur. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
ER  -

ŠIMEČKOVÁ, Pavlína; Josef SLAVÍK; Andrea FOŘTOVÁ; Ivana HUVAROVÁ; Lucie KRÁLIKOVÁ; Michal STEFANIK; Pavel SVOBODA; Daniel RŮŽEK a Miroslav MACHALA. Tick-borne encephalitis virus modulates sphingolipid and phospholipid metabolism in infected human neuronal cells. \textit{Microbes and Infection}. Elsevier, 2024, roč.~26, č.~4, s.~1-11. ISSN~1286-4579. Dostupné z: https://doi.org/10.1016/j.micinf.2024.105303.

Přehled o publikaci