Screening of Glycyrrhiza species for anti-inflammatory phenols

a 2024

Screening of Glycyrrhiza species for anti-inflammatory phenols

MARKUŠ, Juraj; Karel ŠMEJKAL a Jakub TREML

Základní údaje

Originální název

Screening of Glycyrrhiza species for anti-inflammatory phenols

Autoři

MARKUŠ, Juraj; Karel ŠMEJKAL a Jakub TREML

Vydání

PSE Trends in Natural Products 2024 Young Scientists' Meeting: BIOACTIVE NATURAL PRODUCTS IN BIOLOGY, PHARMACY AND COSMETICS, 2024, 2024

Další údaje

Jazyk

angličtina

Typ výsledku

Konferenční abstrakt

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Označené pro přenos do RIV

Organizační jednotka

Farmaceutická fakulta

ISBN

978-80-280-0559-7

Klíčová slova anglicky

glycyrrhiza; phytochemistry; bioactivity;

Štítky

ÚPL

Změněno: 30. 1. 2025 16:53, Mgr. Juraj Markuš

Anotace

V originále

Roots and rhizomes of Glycyrrhiza species (Fabaceae) have long been used for their antiinflammatory, expectorant, and antimicrobial effects. Our research is dedicated to screening and selection of different Glycyrrhiza taxa for further isolation of bioactive compounds with with an emphasis on prenylated phenols. Plant material was obtained by cooperation with Mendel University, Faculty of Horticulture. Raw material from total of thirteen Glycyrrhiza varieties including six species (G. glabra, G. uralensis, G. pallidiflora, G. foetida, G. yunnanensis, and G. echinata) was extracted with ethanol. Extracts were fractionated by liquidliquid extraction (n-hexane, chloroform, ethyl acetate and water). All fractions were then analysed by HPLC-DAD and ELSD. Chloroform and ethyl acetate fractions were tested for cytotoxicity and inhibition of NFκB on THP-1 cells. The major compounds from G. uralensis were isolated by flash chromatography and purified by semipreparative HPLC. HPLC-DAD analysis has not revealed any apparent intraspecific difference in content of phenolic compounds. Structures of isolated compounds will be elucidated by various spectral methods. Identified compounds will undergo further bioassays for anti-inflammatory effects.

Návaznosti

MUNI/A/1414/2023, interní kód MU

Název: Skríning a selekce taxonů Glycyrrhiza species pro obsah protizánětlivých prenylovaných fenolů

Investor: Masarykova univerzita, Skríning a selekce taxonů Glycyrrhiza species pro obsah protizánětlivých prenylovaných fenolů

Citovat

MARKUŠ, Juraj; Karel ŠMEJKAL a Jakub TREML. Screening of Glycyrrhiza species for anti-inflammatory phenols. In PSE Trends in Natural Products 2024 Young Scientists' Meeting: BIOACTIVE NATURAL PRODUCTS IN BIOLOGY, PHARMACY AND COSMETICS, 2024. 2024. ISBN 978-80-280-0559-7.

@proceedings{2472706,
   author = {Markuš, Juraj and Šmejkal, Karel and Treml, Jakub},
   booktitle = {PSE Trends in Natural Products 2024 Young Scientists' Meeting: BIOACTIVE NATURAL PRODUCTS IN BIOLOGY, PHARMACY AND COSMETICS, 2024},
   keywords = {glycyrrhiza; phytochemistry; bioactivity;},
   language = {eng},
   isbn = {978-80-280-0559-7},
   title = {Screening of Glycyrrhiza species for anti-inflammatory phenols},
   year = {2024}
}

TY  - CONF
ID  - 2472706
AU  - Markuš, Juraj - Šmejkal, Karel - Treml, Jakub
PY  - 2024
TI  - Screening of Glycyrrhiza species for anti-inflammatory phenols
SN  - 9788028005597
KW  - glycyrrhiza
KW  - phytochemistry
KW  - bioactivity;
N2  - Roots and rhizomes of Glycyrrhiza species (Fabaceae) have long been used for their antiinflammatory, expectorant, and antimicrobial effects. Our research is dedicated to screening and selection of different Glycyrrhiza taxa for further isolation of bioactive compounds with with an emphasis on prenylated phenols. Plant material was obtained by cooperation with Mendel University, Faculty of Horticulture. Raw material from total of thirteen Glycyrrhiza varieties including six species (G. glabra, G. uralensis, G. pallidiflora, G. foetida, G. yunnanensis, and G. echinata) was extracted with ethanol. Extracts were fractionated by liquidliquid extraction (n-hexane, chloroform, ethyl acetate and water). All fractions were then analysed by HPLC-DAD and ELSD. Chloroform and ethyl acetate fractions were tested for cytotoxicity and inhibition of NFκB on THP-1 cells. The major compounds from G. uralensis were isolated by flash chromatography and purified by semipreparative HPLC. HPLC-DAD analysis has not revealed any apparent intraspecific difference in content of phenolic compounds. Structures of isolated compounds will be elucidated by various spectral methods. Identified compounds will undergo further bioassays for anti-inflammatory effects.
ER  -

MARKUŠ, Juraj; Karel ŠMEJKAL a Jakub TREML. Screening of Glycyrrhiza species for anti-inflammatory phenols. In \textit{PSE Trends in Natural Products 2024 Young Scientists' Meeting: BIOACTIVE NATURAL PRODUCTS IN BIOLOGY, PHARMACY AND COSMETICS, 2024}. 2024. ISBN~978-80-280-0559-7.

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