Exploring the transcriptomic profile of human monkeypox virus
via CAGE and native RNA sequencing approaches

J 2024

Exploring the transcriptomic profile of human monkeypox virus via CAGE and native RNA sequencing approaches

NAGY, Gergely armin; Dora TOMBACZ; Istvan PRAZSAK; Zsolt CSABAI; Akos DORMO et al.

Základní údaje

Originální název

Exploring the transcriptomic profile of human monkeypox virus via CAGE and native RNA sequencing approaches

Autoři

NAGY, Gergely armin; Dora TOMBACZ; Istvan PRAZSAK; Zsolt CSABAI; Akos DORMO; Gabor GULYAS; Gabor KEMENESI; Gabor E TOTH; Jiří HOLOUBEK; Daniel RŮŽEK; Balazs KAKUK a Zsolt BOLDOGKOI

Vydání

mSphere, Washington, DC, USA, American Society for Microbiology, 2024, 2379-5042

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10606 Microbiology

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 3.100

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/24:00138727

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

monkeypox virus; CAGE-Seq; nanopore sequencing; long-read sequencing; transcriptome; poxvirus

Štítky

14314010, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 31. 1. 2025 12:48, Mgr. Marie Novosadová Šípková, DiS.

Anotace

V originále

In this study, we employed short- and long-read sequencing technologies to delineate the transcriptional architecture of the human monkeypox virus and to identify key regulatory elements that govern its gene expression. Specifically, we conducted a transcriptomic analysis to annotate the transcription start sites (TSSs) and transcription end sites (TESs) of the virus by utilizing Cap Analysis of gene expression sequencing on the Illumina platform and direct RNA sequencing on the Oxford Nanopore technology device. Our investigations uncovered significant complexity in the use of alternative TSSs and TESs in viral genes. In this research, we also detected the promoter elements and poly(A) signals associated with the viral genes. Additionally, we identified novel genes in both the left and right variable regions of the viral genome.IMPORTANCEGenerally, gaining insight into how the transcription of a virus is regulated offers insights into the key mechanisms that control its life cycle. The recent outbreak of the human monkeypox virus has underscored the necessity of understanding the basic biology of its causative agent. Our results are pivotal for constructing a comprehensive transcriptomic atlas of the human monkeypox virus, providing valuable resources for future studies.

Citovat

NAGY, Gergely armin; Dora TOMBACZ; Istvan PRAZSAK; Zsolt CSABAI; Akos DORMO; Gabor GULYAS; Gabor KEMENESI; Gabor E TOTH; Jiří HOLOUBEK; Daniel RŮŽEK; Balazs KAKUK a Zsolt BOLDOGKOI. Exploring the transcriptomic profile of human monkeypox virus via CAGE and native RNA sequencing approaches. mSphere. Washington, DC, USA: American Society for Microbiology, 2024, roč. 9, č. 9, s. 1-22. ISSN 2379-5042. Dostupné z: https://doi.org/10.1128/msphere.00356-24.

@article{2472956,
   author = {Nagy, Gergely armin and Tombacz, Dora and Prazsak, Istvan and Csabai, Zsolt and Dormo, Akos and Gulyas, Gabor and Kemenesi, Gabor and Toth, Gabor E and Holoubek, Jiří and Růžek, Daniel and Kakuk, Balazs and Boldogkoi, Zsolt},
   article_location = {Washington, DC, USA},
   article_number = {9},
   doi = {https://doi.org/10.1128/msphere.00356-24},
   keywords = {monkeypox virus; CAGE-Seq; nanopore sequencing; long-read sequencing; transcriptome; poxvirus},
   language = {eng},
   issn = {2379-5042},
   journal = {mSphere},
   title = {Exploring the transcriptomic profile of human monkeypox virus via CAGE and native RNA sequencing approaches},
   url = {https://journals.asm.org/doi/10.1128/msphere.00356-24},
   volume = {9},
   year = {2024}
}

TY  - JOUR
ID  - 2472956
AU  - Nagy, Gergely armin - Tombacz, Dora - Prazsak, Istvan - Csabai, Zsolt - Dormo, Akos - Gulyas, Gabor - Kemenesi, Gabor - Toth, Gabor E - Holoubek, Jiří - Růžek, Daniel - Kakuk, Balazs - Boldogkoi, Zsolt
PY  - 2024
TI  - Exploring the transcriptomic profile of human monkeypox virus via CAGE and native RNA sequencing approaches
JF  - mSphere
VL  - 9
IS  - 9
SP  - 1-22
EP  - 1-22
PB  - American Society for Microbiology
SN  - 23795042
KW  - monkeypox virus
KW  - CAGE-Seq
KW  - nanopore sequencing
KW  - long-read sequencing
KW  - transcriptome
KW  - poxvirus
UR  - https://journals.asm.org/doi/10.1128/msphere.00356-24
N2  - In this study, we employed short- and long-read sequencing technologies to delineate the transcriptional architecture of the human monkeypox virus and to identify key regulatory elements that govern its gene expression. Specifically, we conducted a transcriptomic analysis to annotate the transcription start sites (TSSs) and transcription end sites (TESs) of the virus by utilizing Cap Analysis of gene expression sequencing on the Illumina platform and direct RNA sequencing on the Oxford Nanopore technology device. Our investigations uncovered significant complexity in the use of alternative TSSs and TESs in viral genes. In this research, we also detected the promoter elements and poly(A) signals associated with the viral genes. Additionally, we identified novel genes in both the left and right variable regions of the viral genome.IMPORTANCEGenerally, gaining insight into how the transcription of a virus is regulated offers insights into the key mechanisms that control its life cycle. The recent outbreak of the human monkeypox virus has underscored the necessity of understanding the basic biology of its causative agent. Our results are pivotal for constructing a comprehensive transcriptomic atlas of the human monkeypox virus, providing valuable resources for future studies.
ER  -

NAGY, Gergely armin; Dora TOMBACZ; Istvan PRAZSAK; Zsolt CSABAI; Akos DORMO; Gabor GULYAS; Gabor KEMENESI; Gabor E TOTH; Jiří HOLOUBEK; Daniel RŮŽEK; Balazs KAKUK a Zsolt BOLDOGKOI. Exploring the transcriptomic profile of human monkeypox virus via CAGE and native RNA sequencing approaches. \textit{mSphere}. Washington, DC, USA: American Society for Microbiology, 2024, roč.~9, č.~9, s.~1-22. ISSN~2379-5042. Dostupné z: https://doi.org/10.1128/msphere.00356-24.

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