A New Abscisic Acid Conjugate, ABA-L-Glutamate, Determined in
Different Plant Species by Combined Immunoaffinity
Chromatography-Tandem Mass Spectrometry

J 2024

A New Abscisic Acid Conjugate, ABA-L-Glutamate, Determined in Different Plant Species by Combined Immunoaffinity Chromatography-Tandem Mass Spectrometry

TUREČKOVÁ, Veronika; Jana OKLESTKOVÁ; Asta ŽUKAUSKAITĖ; Luděk EYER; Ondřej NOVÁK et al.

Základní údaje

Originální název

A New Abscisic Acid Conjugate, ABA-L-Glutamate, Determined in Different Plant Species by Combined Immunoaffinity Chromatography-Tandem Mass Spectrometry

Autoři

TUREČKOVÁ, Veronika; Jana OKLESTKOVÁ; Asta ŽUKAUSKAITĖ; Luděk EYER; Ondřej NOVÁK a Miroslav STRNAD

Vydání

Journal of Plant Growth Regulation, Springer, 2024, 0721-7595

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10611 Plant sciences, botany

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 4.400

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/24:00138730

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

Abscisic acid amino acid conjugate (ABA-AA); Enzyme-linked immunosorbent assay (ELISA); Monoclonal antibody (mAb); Immunoaffinity chromatography (IAC); Ultra-high-performance liquid chromatography (UHPLC); Tandem mass spectrometry (MS/MS)

Štítky

14314010, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 31. 1. 2025 14:28, Mgr. Marie Novosadová Šípková, DiS.

Anotace

V originále

Abscisic acid (ABA) is a phytohormone that occurs in plants at very low concentration (pmol/g fresh weight) and regulates multiple biological processes, including stomatal closure, seed germination, and responses to environmental stresses. In the present study, isolation of ABA, ABA glucosyl ester, and 11 ABA amino acid conjugates from minute quantities of plant tissue (less than 20 mg fresh weight) was achieved using a purification method based on the combination of an Oasis HLB column and an immunoaffinity sorbent. New monoclonal antibodies raised against (+)-cis,trans-ABA conjugated to BSA through its carboxyl group (C1) were characterised by enzyme-linked immunosorbent assay (ELISA) and used for immunoaffinity chromatography (IAC) gel preparation. The use of immunoaffinity purification significantly reduced matrix effects and increased the selectivity and sensitivity of subsequent UHPLC-MS/MS analysis. In addition to (+)-cis,trans-ABA and its glucosyl ester, a new abscisic acid conjugate, ABA-L-glutamate, was isolated by IAC and identified by tandem mass spectrometry in pea (Pisum sativum L.), Lepidium sativum L. and wheat (Triticum aestivum L.) seedlings. However, it was not found in 10-day-old seedlings of Arabidopsis thaliana or water-stressed tobacco (Nicotiana tabacum L.) leaves. Here, the identification of an ABA conjugate with glutamic acid in plants is described for the first time.

Citovat

TUREČKOVÁ, Veronika; Jana OKLESTKOVÁ; Asta ŽUKAUSKAITĖ; Luděk EYER; Ondřej NOVÁK a Miroslav STRNAD. A New Abscisic Acid Conjugate, ABA-L-Glutamate, Determined in Different Plant Species by Combined Immunoaffinity Chromatography-Tandem Mass Spectrometry. Journal of Plant Growth Regulation. Springer, 2024, roč. 43, č. 12, s. 4810-4825. ISSN 0721-7595. Dostupné z: https://doi.org/10.1007/s00344-024-11436-2.

@article{2472980,
   author = {Turečková, Veronika and Oklestková, Jana and Žukauskaitė, Asta and Eyer, Luděk and Novák, Ondřej and Strnad, Miroslav},
   article_number = {12},
   doi = {https://doi.org/10.1007/s00344-024-11436-2},
   keywords = {Abscisic acid amino acid conjugate (ABA-AA); Enzyme-linked immunosorbent assay (ELISA); Monoclonal antibody (mAb); Immunoaffinity chromatography (IAC); Ultra-high-performance liquid chromatography (UHPLC); Tandem mass spectrometry (MS/MS)},
   language = {eng},
   issn = {0721-7595},
   journal = {Journal of Plant Growth Regulation},
   title = {A New Abscisic Acid Conjugate, ABA-L-Glutamate, Determined in Different Plant Species by Combined Immunoaffinity Chromatography-Tandem Mass Spectrometry},
   url = {https://link.springer.com/article/10.1007/s00344-024-11436-2},
   volume = {43},
   year = {2024}
}

TY  - JOUR
ID  - 2472980
AU  - Turečková, Veronika - Oklestková, Jana - Žukauskaitė, Asta - Eyer, Luděk - Novák, Ondřej - Strnad, Miroslav
PY  - 2024
TI  - A New Abscisic Acid Conjugate, ABA-L-Glutamate, Determined in Different Plant Species by Combined Immunoaffinity Chromatography-Tandem Mass Spectrometry
JF  - Journal of Plant Growth Regulation
VL  - 43
IS  - 12
SP  - 4810-4825
EP  - 4810-4825
PB  - Springer
SN  - 07217595
KW  - Abscisic acid amino acid conjugate (ABA-AA)
KW  - Enzyme-linked immunosorbent assay (ELISA)
KW  - Monoclonal antibody (mAb)
KW  - Immunoaffinity chromatography (IAC)
KW  - Ultra-high-performance liquid chromatography (UHPLC)
KW  - Tandem mass spectrometry (MS/MS)
UR  - https://link.springer.com/article/10.1007/s00344-024-11436-2
N2  - Abscisic acid (ABA) is a phytohormone that occurs in plants at very low concentration (pmol/g fresh weight) and regulates multiple biological processes, including stomatal closure, seed germination, and responses to environmental stresses. In the present study, isolation of ABA, ABA glucosyl ester, and 11 ABA amino acid conjugates from minute quantities of plant tissue (less than 20 mg fresh weight) was achieved using a purification method based on the combination of an Oasis HLB column and an immunoaffinity sorbent. New monoclonal antibodies raised against (+)-cis,trans-ABA conjugated to BSA through its carboxyl group (C1) were characterised by enzyme-linked immunosorbent assay (ELISA) and used for immunoaffinity chromatography (IAC) gel preparation. The use of immunoaffinity purification significantly reduced matrix effects and increased the selectivity and sensitivity of subsequent UHPLC-MS/MS analysis. In addition to (+)-cis,trans-ABA and its glucosyl ester, a new abscisic acid conjugate, ABA-L-glutamate, was isolated by IAC and identified by tandem mass spectrometry in pea (Pisum sativum L.), Lepidium sativum L. and wheat (Triticum aestivum L.) seedlings. However, it was not found in 10-day-old seedlings of Arabidopsis thaliana or water-stressed tobacco (Nicotiana tabacum L.) leaves. Here, the identification of an ABA conjugate with glutamic acid in plants is described for the first time.
ER  -

TUREČKOVÁ, Veronika; Jana OKLESTKOVÁ; Asta ŽUKAUSKAITĖ; Luděk EYER; Ondřej NOVÁK a Miroslav STRNAD. A New Abscisic Acid Conjugate, ABA-L-Glutamate, Determined in Different Plant Species by Combined Immunoaffinity Chromatography-Tandem Mass Spectrometry. \textit{Journal of Plant Growth Regulation}. Springer, 2024, roč.~43, č.~12, s.~4810-4825. ISSN~0721-7595. Dostupné z: https://doi.org/10.1007/s00344-024-11436-2.

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