Unraveling the role of human microglia in tick-borne
encephalitis virus infection: insights into neuroinflammation
and viral pathogenesis.

J 2024

Unraveling the role of human microglia in tick-borne encephalitis virus infection: insights into neuroinflammation and viral pathogenesis.

PRANCLOVA, Veronika; Lenka NEDVEDOVA; Eliska KOTOUNOVA; Vaclav HÖNIG; Marketa DVORAKOVA et al.

Základní údaje

Originální název

Unraveling the role of human microglia in tick-borne encephalitis virus infection: insights into neuroinflammation and viral pathogenesis.

Autoři

PRANCLOVA, Veronika; Lenka NEDVEDOVA; Eliska KOTOUNOVA; Vaclav HÖNIG; Marketa DVORAKOVA; Marika DAVIDKOVA; Tomas BILY; Marie VANCOVA; Daniel RŮŽEK a Martin PALUS

Vydání

Microbes and Infection, Elsevier, 2024, 1286-4579

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10607 Virology

Stát vydavatele

Nizozemské království

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 2.700

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/24:00138755

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

Tick-borne encephalitis virus; Primary human microglia; Cytokine/chemokine; Neuroinflammation; Viral strains

Štítky

14314010, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 28. 2. 2025 15:21, Mgr. Marie Novosadová Šípková, DiS.

Anotace

V originále

Tick-borne encephalitis virus (TBEV) is a neurotropic orthoflavivirus responsible for severe infections of the central nervous system. Although neurons are predominantly targeted, specific involvement of microglia in pathogenesis of TBE is not yet fully understood. In this study, the susceptibility of human microglia to TBEV is investigated, focusing on productive infection and different immune responses of different viral strains. We investigated primary human microglia and two immortalized microglial cell lines exposed to three TBEV strains (Hypr, Neudörfl and 280), each differing in virulence. Our results show that all microglia cultures tested support long-term productive infections, regardless of the viral strain. In particular, immune response varied significantly with the viral strain, as shown by the differential secretion of cytokines and chemokines such as IP-10, MCP-1, IL-8 and IL-6, quantified using a Luminex 48-plex assay. The most virulent strain triggered the highest cytokine induction. Electron tomography revealed substantial ultrastructural changes in the infected microglia, despite the absence of cytopathic effects. These findings underscore the susceptibility of human microglia to TBEV and reveal strain-dependent variations in viral replication and immune responses, highlighting the complex role of microglia in TBEV-induced neuropathology and contribute to a deeper understanding of TBE pathogenesis and neuroinflammation.

Návaznosti

90250, velká výzkumná infrastruktura

Název: Czech-BioImaging III

Citovat

PRANCLOVA, Veronika; Lenka NEDVEDOVA; Eliska KOTOUNOVA; Vaclav HÖNIG; Marketa DVORAKOVA; Marika DAVIDKOVA; Tomas BILY; Marie VANCOVA; Daniel RŮŽEK a Martin PALUS. Unraveling the role of human microglia in tick-borne encephalitis virus infection: insights into neuroinflammation and viral pathogenesis. Microbes and Infection. Elsevier, 2024, roč. 26, č. 8, s. 1-12. ISSN 1286-4579. Dostupné z: https://doi.org/10.1016/j.micinf.2024.105383.

@article{2473184,
   author = {Pranclova, Veronika and Nedvedova, Lenka and Kotounova, Eliska and Hönig, Vaclav and Dvorakova, Marketa and Davidkova, Marika and Bily, Tomas and Vancova, Marie and Růžek, Daniel and Palus, Martin},
   article_number = {8},
   doi = {https://doi.org/10.1016/j.micinf.2024.105383},
   keywords = {Tick-borne encephalitis virus; Primary human microglia; Cytokine/chemokine; Neuroinflammation; Viral strains},
   language = {eng},
   issn = {1286-4579},
   journal = {Microbes and Infection},
   title = {Unraveling the role of human microglia in tick-borne encephalitis virus infection: insights into neuroinflammation and viral pathogenesis.},
   url = {https://doi.org/10.1016/j.micinf.2024.105383},
   volume = {26},
   year = {2024}
}

TY  - JOUR
ID  - 2473184
AU  - Pranclova, Veronika - Nedvedova, Lenka - Kotounova, Eliska - Hönig, Vaclav - Dvorakova, Marketa - Davidkova, Marika - Bily, Tomas - Vancova, Marie - Růžek, Daniel - Palus, Martin
PY  - 2024
TI  - Unraveling the role of human microglia in tick-borne encephalitis virus infection: insights into neuroinflammation and viral pathogenesis.
JF  - Microbes and Infection
VL  - 26
IS  - 8
SP  - 1-12
EP  - 1-12
PB  - Elsevier
SN  - 12864579
KW  - Tick-borne encephalitis virus
KW  - Primary human microglia
KW  - Cytokine/chemokine
KW  - Neuroinflammation
KW  - Viral strains
UR  - https://doi.org/10.1016/j.micinf.2024.105383
N2  - Tick-borne encephalitis virus (TBEV) is a neurotropic orthoflavivirus responsible for severe infections of the central nervous system. Although neurons are predominantly targeted, specific involvement of microglia in pathogenesis of TBE is not yet fully understood. In this study, the susceptibility of human microglia to TBEV is investigated, focusing on productive infection and different immune responses of different viral strains. We investigated primary human microglia and two immortalized microglial cell lines exposed to three TBEV strains (Hypr, Neudörfl and 280), each differing in virulence. Our results show that all microglia cultures tested support long-term productive infections, regardless of the viral strain. In particular, immune response varied significantly with the viral strain, as shown by the differential secretion of cytokines and chemokines such as IP-10, MCP-1, IL-8 and IL-6, quantified using a Luminex 48-plex assay. The most virulent strain triggered the highest cytokine induction. Electron tomography revealed substantial ultrastructural changes in the infected microglia, despite the absence of cytopathic effects. These findings underscore the susceptibility of human microglia to TBEV and reveal strain-dependent variations in viral replication and immune responses, highlighting the complex role of microglia in TBEV-induced neuropathology and contribute to a deeper understanding of TBE pathogenesis and neuroinflammation.
ER  -

PRANCLOVA, Veronika; Lenka NEDVEDOVA; Eliska KOTOUNOVA; Vaclav HÖNIG; Marketa DVORAKOVA; Marika DAVIDKOVA; Tomas BILY; Marie VANCOVA; Daniel RŮŽEK a Martin PALUS. Unraveling the role of human microglia in tick-borne encephalitis virus infection: insights into neuroinflammation and viral pathogenesis. \textit{Microbes and Infection}. Elsevier, 2024, roč.~26, č.~8, s.~1-12. ISSN~1286-4579. Dostupné z: https://doi.org/10.1016/j.micinf.2024.105383.

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