Nanoformulation of the Broad-Spectrum Hydrophobic Antiviral
Vacuolar ATPase Inhibitor Diphyllin in Human Recombinant
H-ferritin.

J 2024

Nanoformulation of the Broad-Spectrum Hydrophobic Antiviral Vacuolar ATPase Inhibitor Diphyllin in Human Recombinant H-ferritin.

VOJNIKOVA, Michaela; Martina SÚKUPOVÁ; Michal STEFANIK; Petra STRAKOVÁ; Jan HAVIERNIK et al.

Základní údaje

Originální název

Nanoformulation of the Broad-Spectrum Hydrophobic Antiviral Vacuolar ATPase Inhibitor Diphyllin in Human Recombinant H-ferritin.

Autoři

Vydání

International Journal of Nanomedicine, Dove Medical Press Ltd, 2024, 1178-2013

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10607 Virology

Stát vydavatele

Nový Zéland

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 6.500

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/24:00138757

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

drug delivery; SARS-CoV-2; TBEV; WNV; Zika virus

Štítky

14314010, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 17. 3. 2025 14:53, Mgr. Marie Novosadová Šípková, DiS.

Anotace

V originále

Background: As highlighted by recent pandemic outbreaks, antiviral drugs are crucial resources in the global battle against viral diseases. Unfortunately, most antiviral drugs are characterized by a plethora of side effects and low efficiency/poor bioavailability owing to their insolubility. This also applies to the arylnaphthalide lignin family member, diphyllin (Diph). Diph acts as a vacuolar ATPase inhibitor and has been previously identified as a promising candidate with broad-spectrum antiviral activity. However, its physicochemical properties preclude its efficient administration in vivo, complicating preclinical testing. Methods: We produced human recombinant H-ferritin (HsaFtH) and used it as a delivery vehicle for Diph encapsulation through pH-mediated reversible reassembly of HsaFtH. Diph nanoformulation was subsequently thoroughly characterized and tested for its non-target cytotoxicity and antiviral efficiency using a panel of pathogenic viral strain. Results: We revealed that loading into HsaFtH decreased the undesired cytotoxicity of Diph in mammalian host cells. We also confirmed that encapsulated Diph exhibited slightly lower antiviral activity than free Diph, which may be due to the differential uptake mechanism and kinetics of free Diph and Diph@HsaFtH. Furthermore, we confirmed that the antiviral effect was mediated solely by Diph with no contribution from HsaFtH. Conclusion: It was confirmed that HsaFtH is a suitable vehicle that allows easy loading of Diph and production of highly homogeneous nanoparticles dispersion with promising broad-spectrum antiviral activity.

Citovat

VOJNIKOVA, Michaela; Martina SÚKUPOVÁ; Michal STEFANIK; Petra STRAKOVÁ; Jan HAVIERNIK; Katerina KAPOLKOVA; Eliska GRUBEROVA; Klara RASKOVA; Hana MICHALKOVA; Pavel SVEC; Marie PESKOVA KUDLICKOVA; Ivana HUVAROVA; Daniel RŮŽEK; Jiří SALÁT; Vladimir PEKARIK; Luděk EYER a Zbyněk HEGER. Nanoformulation of the Broad-Spectrum Hydrophobic Antiviral Vacuolar ATPase Inhibitor Diphyllin in Human Recombinant H-ferritin. International Journal of Nanomedicine. Dove Medical Press Ltd, 2024, roč. 19, April, s. 3907-3917. ISSN 1178-2013. Dostupné z: https://doi.org/10.2147/IJN.S452119.

@article{2473186,
   author = {Vojnikova, Michaela and Súkupová, Martina and Stefanik, Michal and Straková, Petra and Haviernik, Jan and Kapolkova, Katerina and Gruberova, Eliska and Raskova, Klara and Michalkova, Hana and Svec, Pavel and Peskova Kudlickova, Marie and Huvarova, Ivana and Růžek, Daniel and Salát, Jiří and Pekarik, Vladimir and Eyer, Luděk and Heger, Zbyněk},
   article_number = {April},
   doi = {https://doi.org/10.2147/IJN.S452119},
   keywords = {drug delivery; SARS-CoV-2; TBEV; WNV; Zika virus},
   language = {eng},
   issn = {1178-2013},
   journal = {International Journal of Nanomedicine},
   title = {Nanoformulation of the Broad-Spectrum Hydrophobic Antiviral Vacuolar ATPase Inhibitor Diphyllin in Human Recombinant H-ferritin.},
   url = {https://doi.org/10.2147/IJN.S452119},
   volume = {19},
   year = {2024}
}

TY  - JOUR
ID  - 2473186
AU  - Vojnikova, Michaela - Súkupová, Martina - Stefanik, Michal - Straková, Petra - Haviernik, Jan - Kapolkova, Katerina - Gruberova, Eliska - Raskova, Klara - Michalkova, Hana - Svec, Pavel - Peskova Kudlickova, Marie - Huvarova, Ivana - Růžek, Daniel - Salát, Jiří - Pekarik, Vladimir - Eyer, Luděk - Heger, Zbyněk
PY  - 2024
TI  - Nanoformulation of the Broad-Spectrum Hydrophobic Antiviral Vacuolar ATPase Inhibitor Diphyllin in Human Recombinant H-ferritin.
JF  - International Journal of Nanomedicine
VL  - 19
IS  - April
SP  - 3907-3917
EP  - 3907-3917
PB  - Dove Medical Press Ltd
SN  - 11782013
KW  - drug delivery
KW  - SARS-CoV-2
KW  - TBEV
KW  - WNV
KW  - Zika virus
UR  - https://doi.org/10.2147/IJN.S452119
N2  - Background: As highlighted by recent pandemic outbreaks, antiviral drugs are crucial resources in the global battle against viral diseases. Unfortunately, most antiviral drugs are characterized by a plethora of side effects and low efficiency/poor bioavailability owing to their insolubility. This also applies to the arylnaphthalide lignin family member, diphyllin (Diph). Diph acts as a vacuolar ATPase inhibitor and has been previously identified as a promising candidate with broad-spectrum antiviral activity. However, its physicochemical properties preclude its efficient administration in vivo, complicating preclinical testing. Methods: We produced human recombinant H-ferritin (HsaFtH) and used it as a delivery vehicle for Diph encapsulation through pH-mediated reversible reassembly of HsaFtH. Diph nanoformulation was subsequently thoroughly characterized and tested for its non-target cytotoxicity and antiviral efficiency using a panel of pathogenic viral strain. Results: We revealed that loading into HsaFtH decreased the undesired cytotoxicity of Diph in mammalian host cells. We also confirmed that encapsulated Diph exhibited slightly lower antiviral activity than free Diph, which may be due to the differential uptake mechanism and kinetics of free Diph and Diph@HsaFtH. Furthermore, we confirmed that the antiviral effect was mediated solely by Diph with no contribution from HsaFtH. Conclusion: It was confirmed that HsaFtH is a suitable vehicle that allows easy loading of Diph and production of highly homogeneous nanoparticles dispersion with promising broad-spectrum antiviral activity.
ER  -

VOJNIKOVA, Michaela; Martina SÚKUPOVÁ; Michal STEFANIK; Petra STRAKOVÁ; Jan HAVIERNIK; Katerina KAPOLKOVA; Eliska GRUBEROVA; Klara RASKOVA; Hana MICHALKOVA; Pavel SVEC; Marie PESKOVA KUDLICKOVA; Ivana HUVAROVA; Daniel RŮŽEK; Jiří SALÁT; Vladimir PEKARIK; Luděk EYER a Zbyněk HEGER. Nanoformulation of the Broad-Spectrum Hydrophobic Antiviral Vacuolar ATPase Inhibitor Diphyllin in Human Recombinant H-ferritin. \textit{International Journal of Nanomedicine}. Dove Medical Press Ltd, 2024, roč.~19, April, s.~3907-3917. ISSN~1178-2013. Dostupné z: https://doi.org/10.2147/IJN.S452119.

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