Multicentric evaluation of sensitivity of eight commercial
anti-SARS-CoV-2 antibody assays and their correlation to virus
neutralization titers in seropositive subjects.

J 2024

Multicentric evaluation of sensitivity of eight commercial anti-SARS-CoV-2 antibody assays and their correlation to virus neutralization titers in seropositive subjects.

FAJFR, Miroslav; Petr PAJER; Daniel RŮŽEK; Radek SLEHA; Sylva JANOVSKA et al.

Základní údaje

Originální název

Multicentric evaluation of sensitivity of eight commercial anti-SARS-CoV-2 antibody assays and their correlation to virus neutralization titers in seropositive subjects.

Autoři

FAJFR, Miroslav; Petr PAJER; Daniel RŮŽEK; Radek SLEHA; Sylva JANOVSKA; Milos BOHONEK; Hana KABICKOVA; Pavla KUBICKOVÁ; Michal STEFANIK; Petra STRAKOVÁ a Pavel BOSTIK

Vydání

Scientific Reports, Nature Research, 2024, 2045-2322

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10607 Virology

Stát vydavatele

Německo

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 3.900

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/24:00138758

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

Clinical microbiology; Infectious-disease diagnostics; SARS-CoV-2

Štítky

14314010, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 17. 3. 2025 14:59, Mgr. Marie Novosadová Šípková, DiS.

Anotace

V originále

Diagnosis of SARS-CoV-2 virus is mainly based on direct detection. Determination of specific antibodies has been used mostly for epidemiological reasons. However, select immunoassays showed good correlation to plaque reduction virus neutralization test (PRNT) in smaller patient cohorts, which suggests their potential as predictors of virus neutralization titer. A total of 3,699 samples from Covid-19 patients were included in the multicentric study performed in the Czech Republic. Anti-SARS-CoV-2 antibody levels were evaluated by 8 commercial antibody assays. Simultaneously, PRNT evaluations were performed with the SARS-CoV-2 B.1.258 variant. All immunoassays showed an overall high true positive diagnostic value ranging from 79.17 to 98.04%. Several commercial EIA methods showed highly positive correlation between the assay results and PRNT levels, e.g., Liaison CoV-2 TrimericS IgG DiaSorin (Spearman r = 0.8833; Architect SASRS-CoV-2 IgG Abbott (r = 0.7298); NovaLisa SARS-CoV-2 IgG NovaTec (r = 0.7103) and Anti-SARS-CoV-2 ELISA IgG Euroimmun (r = 0.7094). While this correlation was less positive for other assays, those, conversely, presented higher true positive values. For most immunoassays, the positive percent agreement of the results was ≥ 95% in sera exhibiting PRNT levels of 1:80 and higher. The assays tested have shown variable correlation to PRNT. Those possessing high positive predictive values serve well as qualitative tests, while others can be utilised as quantitative tests highly predictive of neutralization antibody levels.

Citovat

FAJFR, Miroslav; Petr PAJER; Daniel RŮŽEK; Radek SLEHA; Sylva JANOVSKA; Milos BOHONEK; Hana KABICKOVA; Pavla KUBICKOVÁ; Michal STEFANIK; Petra STRAKOVÁ a Pavel BOSTIK. Multicentric evaluation of sensitivity of eight commercial anti-SARS-CoV-2 antibody assays and their correlation to virus neutralization titers in seropositive subjects. Scientific Reports. Nature Research, 2024, roč. 14, č. 1, s. 1-8. ISSN 2045-2322. Dostupné z: https://doi.org/10.1038/s41598-024-51968-x.

@article{2473187,
   author = {Fajfr, Miroslav and Pajer, Petr and Růžek, Daniel and Sleha, Radek and Janovska, Sylva and Bohonek, Milos and Kabickova, Hana and Kubicková, Pavla and Stefanik, Michal and Straková, Petra and Bostik, Pavel},
   article_number = {1},
   doi = {https://doi.org/10.1038/s41598-024-51968-x},
   keywords = {Clinical microbiology; Infectious-disease diagnostics; SARS-CoV-2},
   language = {eng},
   issn = {2045-2322},
   journal = {Scientific Reports},
   title = {Multicentric evaluation of sensitivity of eight commercial anti-SARS-CoV-2 antibody assays and their correlation to virus neutralization titers in seropositive subjects.},
   url = {https://doi.org/10.1038/s41598-024-51968-x},
   volume = {14},
   year = {2024}
}

TY  - JOUR
ID  - 2473187
AU  - Fajfr, Miroslav - Pajer, Petr - Růžek, Daniel - Sleha, Radek - Janovska, Sylva - Bohonek, Milos - Kabickova, Hana - Kubicková, Pavla - Stefanik, Michal - Straková, Petra - Bostik, Pavel
PY  - 2024
TI  - Multicentric evaluation of sensitivity of eight commercial anti-SARS-CoV-2 antibody assays and their correlation to virus neutralization titers in seropositive subjects.
JF  - Scientific Reports
VL  - 14
IS  - 1
SP  - 1-8
EP  - 1-8
PB  - Nature Research
SN  - 20452322
KW  - Clinical microbiology
KW  - Infectious-disease diagnostics
KW  - SARS-CoV-2
UR  - https://doi.org/10.1038/s41598-024-51968-x
N2  - Diagnosis of SARS-CoV-2 virus is mainly based on direct detection. Determination of specific antibodies has been used mostly for epidemiological reasons. However, select immunoassays showed good correlation to plaque reduction virus neutralization test (PRNT) in smaller patient cohorts, which suggests their potential as predictors of virus neutralization titer. A total of 3,699 samples from Covid-19 patients were included in the multicentric study performed in the Czech Republic. Anti-SARS-CoV-2 antibody levels were evaluated by 8 commercial antibody assays. Simultaneously, PRNT evaluations were performed with the SARS-CoV-2 B.1.258 variant. All immunoassays showed an overall high true positive diagnostic value ranging from 79.17 to 98.04%. Several commercial EIA methods showed highly positive correlation between the assay results and PRNT levels, e.g., Liaison CoV-2 TrimericS IgG DiaSorin (Spearman r = 0.8833; Architect SASRS-CoV-2 IgG Abbott (r = 0.7298); NovaLisa SARS-CoV-2 IgG NovaTec (r = 0.7103) and Anti-SARS-CoV-2 ELISA IgG Euroimmun (r = 0.7094). While this correlation was less positive for other assays, those, conversely, presented higher true positive values. For most immunoassays, the positive percent agreement of the results was ≥ 95% in sera exhibiting PRNT levels of 1:80 and higher. The assays tested have shown variable correlation to PRNT. Those possessing high positive predictive values serve well as qualitative tests, while others can be utilised as quantitative tests highly predictive of neutralization antibody levels.
ER  -

FAJFR, Miroslav; Petr PAJER; Daniel RŮŽEK; Radek SLEHA; Sylva JANOVSKA; Milos BOHONEK; Hana KABICKOVA; Pavla KUBICKOVÁ; Michal STEFANIK; Petra STRAKOVÁ a Pavel BOSTIK. Multicentric evaluation of sensitivity of eight commercial anti-SARS-CoV-2 antibody assays and their correlation to virus neutralization titers in seropositive subjects. \textit{Scientific Reports}. Nature Research, 2024, roč.~14, č.~1, s.~1-8. ISSN~2045-2322. Dostupné z: https://doi.org/10.1038/s41598-024-51968-x.

Přehled o publikaci