Pseudomonas rossensis sp. nov., a novel psychrotolerant species
produces antimicrobial agents targeting resistant clinical
isolates of Pseudomonas aeruginosa

J 2025

Pseudomonas rossensis sp. nov., a novel psychrotolerant species produces antimicrobial agents targeting resistant clinical isolates of Pseudomonas aeruginosa

SNOPKOVÁ, Kateřina; Karel SEDLÁŘ; Dana NOVÁKOVÁ; Eva STAŇKOVÁ; Ivo SEDLÁČEK et. al.

Základní údaje

Originální název

Pseudomonas rossensis sp. nov., a novel psychrotolerant species produces antimicrobial agents targeting resistant clinical isolates of Pseudomonas aeruginosa

Autoři

SNOPKOVÁ, Kateřina; Karel SEDLÁŘ; Dana NOVÁKOVÁ; Eva STAŇKOVÁ; Ivo SEDLÁČEK; Ondrej ŠEDO a Veronika HOLÁ

Vydání

Current Research in Microbial Sciences, Amsterdam, Elsevier B.V. 2025, 2666-5174

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10606 Microbiology

Stát vydavatele

Nizozemské království

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 5.800 v roce 2024

Organizační jednotka

Lékařská fakulta

DOI

https://doi.org/10.1016/j.crmicr.2025.100353

UT WoS

001420704200001

EID Scopus

2-s2.0-85216458337

Klíčová slova anglicky

Antarctica; Novel species; Antimicrobials; Antibiotics; Inhibition; Psychrotolerant bacteria; Cold adaptation

Štítky

14110113, 14314010, podil, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 28. 2. 2025 14:04, Mgr. Tereza Miškechová

Anotace

V originále

The extreme conditions of the Antarctic environment have driven the evolution of highly specialized microbial communities with unique adaptations. In this study, we characterized five Pseudomonas isolates from James Ross Island, which displayed notable taxonomic and metabolite features. Phylogenomic analysis revealed that strain P2663T occupies a distinct phylogenetic position within the Pseudomonas genus, related to species Pseudomonas svalbardensis, Pseudomonas silesiensis, Pseudomonas mucoides, Pseudomonas prosekii, and Pseudomonas gregormendelii. The novelty of five Antarctic isolates was further confirmed through analyses of housekeeping genes, ribotyping, and REP-PCR profiling. MALDI-TOF MS analysis identified 11 unique mass spectrometry signals shared by the Antarctic isolates, which were not detected in other related species. Additionally, chemotaxonomic characterization, including fatty acid composition, demonstrated similarities with related Pseudomonas species. Phenotypic assessments revealed distinctive biochemical and physiological traits. In-depth genomic analysis of strain P2663T uncovered numerous genes which could be involved in survival in extreme Antarctic conditions, including those encoding cold-shock and heat-shock proteins, oxidative and osmotic stress response proteins, and carotenoid-like pigments. Genome mining further revealed several biosynthetic gene clusters, some of which are associated with antimicrobial activity. Functional assays supported the antimicrobial capabilities of this novel species, showing antagonistic effects against clinical isolates of Pseudomonas aeruginosa, possibly mediated by tailocins (phage tail-like particles). This comprehensive polyphasic study characterized a new cold-adapted species, for which we propose the name Pseudomonas rossensis sp. nov.

Návaznosti

EF18_046/0015974, projekt VaV

Název: Modernizace České infrastruktury pro integrativní strukturní biologii

LM2023042, projekt VaV

Název: Česká infrastruktura pro integrativní strukturní biologii

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, CIISB - Česká infrastruktura pro integrativní strukturní biologii

NU22-05-00110, projekt VaV

Název: Polymikrobiální podstata infekcí nehojících se ran a terapeutický potenciál konceptu založeného na 3D biotisku pro léčbu těchto infekcí

Investor: Ministerstvo zdravotnictví ČR, Polymikrobiální podstata infekcí nehojících se ran a terapeutický potenciál konceptu založeného na 3D biotisku pro léčbu těchto infekcí, Podprogram 1 - standardní

Citovat

SNOPKOVÁ, Kateřina; Karel SEDLÁŘ; Dana NOVÁKOVÁ; Eva STAŇKOVÁ; Ivo SEDLÁČEK; Ondrej ŠEDO a Veronika HOLÁ. Pseudomonas rossensis sp. nov., a novel psychrotolerant species produces antimicrobial agents targeting resistant clinical isolates of Pseudomonas aeruginosa. Current Research in Microbial Sciences. Amsterdam: Elsevier B.V., 2025, roč. 8, č. 2025, s. 1-14. ISSN 2666-5174. Dostupné z: https://doi.org/10.1016/j.crmicr.2025.100353.

@article{2474704,
   author = {Snopková, Kateřina and Sedlář, Karel and Nováková, Dana and Staňková, Eva and Sedláček, Ivo and Šedo, Ondrej and Holá, Veronika},
   article_location = {Amsterdam},
   article_number = {2025},
   doi = {https://doi.org/10.1016/j.crmicr.2025.100353},
   keywords = {Antarctica; Novel species; Antimicrobials; Antibiotics; Inhibition; Psychrotolerant bacteria; Cold adaptation},
   language = {eng},
   issn = {2666-5174},
   journal = {Current Research in Microbial Sciences},
   title = {Pseudomonas rossensis sp. nov., a novel psychrotolerant species produces antimicrobial agents targeting resistant clinical isolates of Pseudomonas aeruginosa},
   url = {https://www.sciencedirect.com/science/article/pii/S266651742500015X},
   volume = {8},
   year = {2025}
}

TY  - JOUR
ID  - 2474704
AU  - Snopková, Kateřina - Sedlář, Karel - Nováková, Dana - Staňková, Eva - Sedláček, Ivo - Šedo, Ondrej - Holá, Veronika
PY  - 2025
TI  - Pseudomonas rossensis sp. nov., a novel psychrotolerant species produces antimicrobial agents targeting resistant clinical isolates of Pseudomonas aeruginosa
JF  - Current Research in Microbial Sciences
VL  - 8
IS  - 2025
SP  - 1-14
EP  - 1-14
PB  - Elsevier B.V.
SN  - 26665174
KW  - Antarctica
KW  - Novel species
KW  - Antimicrobials
KW  - Antibiotics
KW  - Inhibition
KW  - Psychrotolerant bacteria
KW  - Cold adaptation
UR  - https://www.sciencedirect.com/science/article/pii/S266651742500015X
N2  - The extreme conditions of the Antarctic environment have driven the evolution of highly specialized microbial communities with unique adaptations. In this study, we characterized five Pseudomonas isolates from James Ross Island, which displayed notable taxonomic and metabolite features. Phylogenomic analysis revealed that strain P2663T occupies a distinct phylogenetic position within the Pseudomonas genus, related to species Pseudomonas svalbardensis, Pseudomonas silesiensis, Pseudomonas mucoides, Pseudomonas prosekii, and Pseudomonas gregormendelii. The novelty of five Antarctic isolates was further confirmed through analyses of housekeeping genes, ribotyping, and REP-PCR profiling. MALDI-TOF MS analysis identified 11 unique mass spectrometry signals shared by the Antarctic isolates, which were not detected in other related species. Additionally, chemotaxonomic characterization, including fatty acid composition, demonstrated similarities with related Pseudomonas species. Phenotypic assessments revealed distinctive biochemical and physiological traits. In-depth genomic analysis of strain P2663T uncovered numerous genes which could be involved in survival in extreme Antarctic conditions, including those encoding cold-shock and heat-shock proteins, oxidative and osmotic stress response proteins, and carotenoid-like pigments. Genome mining further revealed several biosynthetic gene clusters, some of which are associated with antimicrobial activity. Functional assays supported the antimicrobial capabilities of this novel species, showing antagonistic effects against clinical isolates of Pseudomonas aeruginosa, possibly mediated by tailocins (phage tail-like particles). This comprehensive polyphasic study characterized a new cold-adapted species, for which we propose the name Pseudomonas rossensis sp. nov.
ER  -

SNOPKOVÁ, Kateřina; Karel SEDLÁŘ; Dana NOVÁKOVÁ; Eva STAŇKOVÁ; Ivo SEDLÁČEK; Ondrej ŠEDO a Veronika HOLÁ. Pseudomonas rossensis sp. nov., a novel psychrotolerant species produces antimicrobial agents targeting resistant clinical isolates of Pseudomonas aeruginosa. \textit{Current Research in Microbial Sciences}. Amsterdam: Elsevier B.V., 2025, roč.~8, č.~2025, s.~1-14. ISSN~2666-5174. Dostupné z: https://doi.org/10.1016/j.crmicr.2025.100353.

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