Efficient derivation of functional astrocytes from human
induced pluripotent stem cells (hiPSCs)

J 2024

Efficient derivation of functional astrocytes from human induced pluripotent stem cells (hiPSCs)

SZEKY, Balazs; Veronika JURÁKOVÁ; Eliska FOUSKOVA; Anita FEHER; Melinda ZANA et al.

Základní údaje

Originální název

Efficient derivation of functional astrocytes from human induced pluripotent stem cells (hiPSCs)

Autoři

Vydání

Plos one, San Francisco, Public Library of Science, 2024, 1932-6203

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30103 Neurosciences

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 2.600

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/24:00138840

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

Astrocytes; Cell differentiation; Cytokines; Gene expression; Stem cells; Calcium signaling; Calcium imaging; Secretion

Štítky

14313050, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 10. 3. 2025 15:19, Mgr. Marie Novosadová Šípková, DiS.

Anotace

V originále

Astrocytes are specialized glial cell types of the central nervous system (CNS) with remarkably high abundance, morphological and functional diversity. Astrocytes maintain neural metabolic support, synapse regulation, blood-brain barrier integrity and immunological homeostasis through intricate interactions with other cells, including neurons, microglia, pericytes and lymphocytes. Due to their extensive intercellular crosstalks, astrocytes are also implicated in the pathogenesis of CNS disorders, such as ALS (amyotrophic lateral sclerosis), Parkinson's disease and Alzheimer's disease. Despite the critical importance of astrocytes in neurodegeneration and neuroinflammation are recognized, the lack of suitable in vitro systems limits their availability for modeling human brain pathologies. Here, we report the time-efficient, reproducible generation of astrocytes from human induced pluripotent stem cells (hiPSCs). Our hiPSC-derived astrocytes expressed characteristic astrocyte markers, such as GFAP, S100b, ALDH1L1 and AQP4. Furthermore, hiPSC-derived astrocytes displayed spontaneous calcium transients and responded to inflammatory stimuli by the secretion of type A1 and type A2 astrocyte-related cytokines.

Návaznosti

MUNI/A/1582/2023, interní kód MU

Název: Podpora biochemického výzkumu v roce 2024

Investor: Masarykova univerzita, Podpora biochemického výzkumu v roce 2024

Citovat

SZEKY, Balazs; Veronika JURÁKOVÁ; Eliska FOUSKOVA; Anita FEHER; Melinda ZANA; Vivien Reka KARL; Janos FARKAS; Maria BODI-JAKUS; Martina ZAPLETALOVÁ; Shashank PANDEY; Radek KUČERA; Jan LOCHMAN a Andras DINNYES. Efficient derivation of functional astrocytes from human induced pluripotent stem cells (hiPSCs). Plos one. San Francisco: Public Library of Science, 2024, roč. 19, č. 12, s. 1-19. ISSN 1932-6203. Dostupné z: https://doi.org/10.1371/journal.pone.0313514.

@article{2476185,
   author = {Szeky, Balazs and Juráková, Veronika and Fouskova, Eliska and Feher, Anita and Zana, Melinda and Karl, Vivien Reka and Farkas, Janos and BodiandJakus, Maria and Zapletalová, Martina and Pandey, Shashank and Kučera, Radek and Lochman, Jan and Dinnyes, Andras},
   article_location = {San Francisco},
   article_number = {12},
   doi = {https://doi.org/10.1371/journal.pone.0313514},
   keywords = {Astrocytes; Cell differentiation; Cytokines; Gene expression; Stem cells; Calcium signaling; Calcium imaging; Secretion},
   language = {eng},
   issn = {1932-6203},
   journal = {Plos one},
   title = {Efficient derivation of functional astrocytes from human induced pluripotent stem cells (hiPSCs)},
   url = {https://doi.org/10.1371/journal.pone.0313514},
   volume = {19},
   year = {2024}
}

TY  - JOUR
ID  - 2476185
AU  - Szeky, Balazs - Juráková, Veronika - Fouskova, Eliska - Feher, Anita - Zana, Melinda - Karl, Vivien Reka - Farkas, Janos - Bodi-Jakus, Maria - Zapletalová, Martina - Pandey, Shashank - Kučera, Radek - Lochman, Jan - Dinnyes, Andras
PY  - 2024
TI  - Efficient derivation of functional astrocytes from human induced pluripotent stem cells (hiPSCs)
JF  - Plos one
VL  - 19
IS  - 12
SP  - 1-19
EP  - 1-19
PB  - Public Library of Science
SN  - 19326203
KW  - Astrocytes
KW  - Cell differentiation
KW  - Cytokines
KW  - Gene expression
KW  - Stem cells
KW  - Calcium signaling
KW  - Calcium imaging
KW  - Secretion
UR  - https://doi.org/10.1371/journal.pone.0313514
N2  - Astrocytes are specialized glial cell types of the central nervous system (CNS) with remarkably high abundance, morphological and functional diversity. Astrocytes maintain neural metabolic support, synapse regulation, blood-brain barrier integrity and immunological homeostasis through intricate interactions with other cells, including neurons, microglia, pericytes and lymphocytes. Due to their extensive intercellular crosstalks, astrocytes are also implicated in the pathogenesis of CNS disorders, such as ALS (amyotrophic lateral sclerosis), Parkinson's disease and Alzheimer's disease. Despite the critical importance of astrocytes in neurodegeneration and neuroinflammation are recognized, the lack of suitable in vitro systems limits their availability for modeling human brain pathologies. Here, we report the time-efficient, reproducible generation of astrocytes from human induced pluripotent stem cells (hiPSCs). Our hiPSC-derived astrocytes expressed characteristic astrocyte markers, such as GFAP, S100b, ALDH1L1 and AQP4. Furthermore, hiPSC-derived astrocytes displayed spontaneous calcium transients and responded to inflammatory stimuli by the secretion of type A1 and type A2 astrocyte-related cytokines.
ER  -

SZEKY, Balazs; Veronika JURÁKOVÁ; Eliska FOUSKOVA; Anita FEHER; Melinda ZANA; Vivien Reka KARL; Janos FARKAS; Maria BODI-JAKUS; Martina ZAPLETALOVÁ; Shashank PANDEY; Radek KUČERA; Jan LOCHMAN a Andras DINNYES. Efficient derivation of functional astrocytes from human induced pluripotent stem cells (hiPSCs). \textit{Plos one}. San Francisco: Public Library of Science, 2024, roč.~19, č.~12, s.~1-19. ISSN~1932-6203. Dostupné z: https://doi.org/10.1371/journal.pone.0313514.

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