Characterization of Heterorhabditis bacteriophora response to
insect-derived and non-biological stimuli: Insights into
nematode recovery and released proteins

J 2025

Characterization of Heterorhabditis bacteriophora response to insect-derived and non-biological stimuli: Insights into nematode recovery and released proteins

ŠREIBR, Sara; Jana ILGOVÁ; Pavel DOBEŠ; Jiří VOREL; Jacek MARCINIAK et al.

Základní údaje

Originální název

Characterization of Heterorhabditis bacteriophora response to insect-derived and non-biological stimuli: Insights into nematode recovery and released proteins

Autoři

ŠREIBR, Sara; Jana ILGOVÁ; Pavel DOBEŠ; Jiří VOREL; Jacek MARCINIAK; Jana HURYCHOVÁ; Duarte TOUBARRO; Nelson SIMÕES; Martin KAŠNÝ a Pavel HYRŠL

Vydání

Pesticide Biochemistry and Physiology, Elsevier Inc. 2025, 0048-3575

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10608 Biochemistry and molecular biology

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 4.000 v roce 2024

Organizační jednotka

Přírodovědecká fakulta

DOI

https://doi.org/10.1016/j.pestbp.2025.106318

UT WoS

001425185300001

EID Scopus

2-s2.0-85217005822

Klíčová slova anglicky

entomopathogenic nematode; Heterorhabditis bacteriophora; recovery; activation; excreted/secreted products; host-derived stimulus; proteomics; secretome

Štítky

14314010, 14314020, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 12. 3. 2025 13:29, Mgr. Marie Novosadová Šípková, DiS.

Anotace

V originále

Entomopathogenic nematodes (EPNs) are biological control agents that naturally kill insect pests, providing an eco-friendly alternative to chemical pesticides. Despite extensive research, the mechanisms behind the recovery process, where infective juveniles (IJs) transition to a parasitic state upon contact with the host, remain unclear. This study investigates the stimulatory effect of insect-derived materials on the recovery of Heterorhabditis bacteriophora IJs. Three materials from Galleria mellonella larvae—bioactive homogenates from live and frozen larvae, and heat-inactivated homogenate—were tested, along with non-host stimuli including filtered water and phosphate-buffered saline (PBS). While none of the materials induced complete recovery of IJs, all triggered the release of excreted/secreted products (ESPs), with consistent protein concentrations across treatments. However, mass spectrometry revealed significant differences in ESP protein composition. IJs exposed to PBS released the highest number of proteins, while bioactive homogenates induced the fewest. Proteins linked to host-parasite interactions, such as alpha-2-macroglobulins and trypsin inhibitor-like proteins, were more abundant in ESPs following exposure to insect-derived materials and PBS. Interestingly, nematodes exposed to water released a substantial number of proteins, comparable to stimulation by heat-inactivated homogenates, though their protein profiles were distinct, reflecting stress responses in the former and host-parasite interaction-related proteins in the latter. Our findings demonstrate that both host-derived and non-biological stimuli can trigger IJs recovery and ESPs release, underscoring the complexity of host-nematode interactions. These results provide novel insights into molecular mechanisms underlying H. bacteriophora parasitism and may contribute to optimizing biocontrol strategies through a better understanding of nematode activation and released ESPs.

Návaznosti

GA23-06457S, projekt VaV

Název: Identifikace a funkční charakteristika bioaktivních molekul produkovaných entomopatogenními hlísticemi

Investor: Grantová agentura ČR, Identifikace a funkční charakteristika bioaktivních molekul produkovaných entomopatogenními hlísticemi

LM2023042, projekt VaV

Název: Česká infrastruktura pro integrativní strukturní biologii

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, CIISB - Česká infrastruktura pro integrativní strukturní biologii

90254, velká výzkumná infrastruktura

Název: e-INFRA CZ II

90255, velká výzkumná infrastruktura

Název: ELIXIR CZ III

Citovat

ŠREIBR, Sara; Jana ILGOVÁ; Pavel DOBEŠ; Jiří VOREL; Jacek MARCINIAK; Jana HURYCHOVÁ; Duarte TOUBARRO; Nelson SIMÕES; Martin KAŠNÝ a Pavel HYRŠL. Characterization of Heterorhabditis bacteriophora response to insect-derived and non-biological stimuli: Insights into nematode recovery and released proteins. Pesticide Biochemistry and Physiology. Elsevier Inc., 2025, roč. 208, March, s. 1-14. ISSN 0048-3575. Dostupné z: https://doi.org/10.1016/j.pestbp.2025.106318.

@article{2476438,
   author = {Šreibr, Sara and Ilgová, Jana and Dobeš, Pavel and Vorel, Jiří and Marciniak, Jacek and Hurychová, Jana and Toubarro, Duarte and Simões, Nelson and Kašný, Martin and Hyršl, Pavel},
   article_number = {March},
   doi = {https://doi.org/10.1016/j.pestbp.2025.106318},
   keywords = {entomopathogenic nematode; Heterorhabditis bacteriophora; recovery; activation; excreted/secreted products; host-derived stimulus; proteomics; secretome},
   language = {eng},
   issn = {0048-3575},
   journal = {Pesticide Biochemistry and Physiology},
   title = {Characterization of Heterorhabditis bacteriophora response to insect-derived and non-biological stimuli: Insights into nematode recovery and released proteins},
   url = {https://www.sciencedirect.com/science/article/pii/S0048357525000318},
   volume = {208},
   year = {2025}
}

TY  - JOUR
ID  - 2476438
AU  - Šreibr, Sara - Ilgová, Jana - Dobeš, Pavel - Vorel, Jiří - Marciniak, Jacek - Hurychová, Jana - Toubarro, Duarte - Simões, Nelson - Kašný, Martin - Hyršl, Pavel
PY  - 2025
TI  - Characterization of Heterorhabditis bacteriophora response to insect-derived and non-biological stimuli: Insights into nematode recovery and released proteins
JF  - Pesticide Biochemistry and Physiology
VL  - 208
IS  - March
SP  - 1-14
EP  - 1-14
PB  - Elsevier Inc.
SN  - 00483575
KW  - entomopathogenic nematode
KW  - Heterorhabditis bacteriophora
KW  - recovery
KW  - activation
KW  - excreted/secreted products
KW  - host-derived stimulus
KW  - proteomics
KW  - secretome
UR  - https://www.sciencedirect.com/science/article/pii/S0048357525000318
N2  - Entomopathogenic nematodes (EPNs) are biological control agents that naturally kill insect pests, providing an eco-friendly alternative to chemical pesticides. Despite extensive research, the mechanisms behind the recovery process, where infective juveniles (IJs) transition to a parasitic state upon contact with the host, remain unclear. This study investigates the stimulatory effect of insect-derived materials on the recovery of Heterorhabditis bacteriophora IJs. Three materials from Galleria mellonella larvae—bioactive homogenates from live and frozen larvae, and heat-inactivated homogenate—were tested, along with non-host stimuli including filtered water and phosphate-buffered saline (PBS). While none of the materials induced complete recovery of IJs, all triggered the release of excreted/secreted products (ESPs), with consistent protein concentrations across treatments. However, mass spectrometry revealed significant differences in ESP protein composition. IJs exposed to PBS released the highest number of proteins, while bioactive homogenates induced the fewest. Proteins linked to host-parasite interactions, such as alpha-2-macroglobulins and trypsin inhibitor-like proteins, were more abundant in ESPs following exposure to insect-derived materials and PBS. Interestingly, nematodes exposed to water released a substantial number of proteins, comparable to stimulation by heat-inactivated homogenates, though their protein profiles were distinct, reflecting stress responses in the former and host-parasite interaction-related proteins in the latter. Our findings demonstrate that both host-derived and non-biological stimuli can trigger IJs recovery and ESPs release, underscoring the complexity of host-nematode interactions. These results provide novel insights into molecular mechanisms underlying H. bacteriophora parasitism and may contribute to optimizing biocontrol strategies through a better understanding of nematode activation and released ESPs.
ER  -

ŠREIBR, Sara; Jana ILGOVÁ; Pavel DOBEŠ; Jiří VOREL; Jacek MARCINIAK; Jana HURYCHOVÁ; Duarte TOUBARRO; Nelson SIMÕES; Martin KAŠNÝ a Pavel HYRŠL. Characterization of Heterorhabditis bacteriophora response to insect-derived and non-biological stimuli: Insights into nematode recovery and released proteins. \textit{Pesticide Biochemistry and Physiology}. Elsevier Inc., 2025, roč.~208, March, s.~1-14. ISSN~0048-3575. Dostupné z: https://doi.org/10.1016/j.pestbp.2025.106318.

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