Amplification of nuclear markers of early medieval pig ancient DNA from south Moravia

FALK, Lenka; Dominika VOJTECHOVÁ; Irena VRTKOVÁ a Eva DROZDOVÁ. Amplification of nuclear markers of early medieval pig ancient DNA from south Moravia. Research in Pig Breeding. Institute of Animal Science, 2024, roč. 18, č. 2, s. 1-6. ISSN 1802-7547.

Základní údaje

Originální název

Amplification of nuclear markers of early medieval pig ancient DNA from south Moravia

Autoři

FALK, Lenka; Dominika VOJTECHOVÁ; Irena VRTKOVÁ a Eva DROZDOVÁ

Vydání

Research in Pig Breeding, Institute of Animal Science, 2024, 1802-7547

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Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

40200 4.2 Animal and Dairy science

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/24:00138856

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

pig aDNA; ancient DNA; Middle Ages; PCR

Štítky

14314010, rivok

Příznaky

Mezinárodní význam, Recenzováno

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Anotace

V originále

Ancient DNA (aDNA) is a DNA molecule older than 75 years. This molecule is characterized by many special properties. The main characteristics include its fragmentation. The short fragment length is often a limitation of the analysis. In this study, we analyzed historical animal samples from the Pohansko site (South Moravia, Czech Republic), which is dated to the 10th century. Two pig tooth samples were selected for aDNA extraction. After successful isolation, three nuclear DNA markers were amplified by PCR. These markers were selected due to the length of their amplification products (134 bp, 209 bp, and 312 bp, respectively). The amplification of the markers was successful for the HAL gene in one sample, and for the TEAD3 gene in both samples, the CYP2E1 gene was not successfully amplified in any sample.