TP53 detection based on electrochemical genosensors with
different types of gold nanoparticles

J 2025

TP53 detection based on electrochemical genosensors with different types of gold nanoparticles

BIRGUSOVA, Eliska; Jiří NAVRÁTIL; Eliska DOSTALOVA; Amir M ASHRAFI; Zuzana BYTESNIKOVA et al.

Základní údaje

Originální název

TP53 detection based on electrochemical genosensors with different types of gold nanoparticles

Autoři

BIRGUSOVA, Eliska; Jiří NAVRÁTIL ; Eliska DOSTALOVA; Amir M ASHRAFI; Zuzana BYTESNIKOVA; Jan PŘIBYL a Lukas RICHTERA

Vydání

Microchemical Journal, AMSTERDAM, Elsevier, 2025, 0026-265X

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10406 Analytical chemistry

Stát vydavatele

Nizozemské království

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 5.100 v roce 2024

Označené pro přenos do RIV

Ano

Organizační jednotka

Lékařská fakulta

Klíčová slova anglicky

Electrochemical genosensor; Gold-based nanomaterials; Diagnostic tool; TP53; P53

Štítky

14110518, CF NANO

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 2. 2. 2026 12:00, Mgr. Eva Suchánková

Anotace

V originále

The TP53 gene, a critical tumour suppressor, is mutated in about half of human cancer cases. Current detection methods are costly and time-consuming. We introduce a label-free electrochemical genosensor for rapid and sensitive TP53 gene detection. Electrode modification with various Au-based nanomaterials, including AuNPs, 3D-mu flowers, and Au-rGO, was investigated. The developed genosensors showed high selectivity and a low LOD of 0.16 pM. Precision was confirmed with RSD% values of 4.63 % (repeatability) and 4.74 % (reproducibility). Above all, we focused on individual materials and their properties when measuring the TP53 gene for future applications. The genosensor operates effectively within the physiological concentration range of 50 to 100 nM, offering a promising tool for cancer diagnostics.

Návaznosti

EF18_046/0015974, projekt VaV

Název: Modernizace České infrastruktury pro integrativní strukturní biologii

LM2023042, projekt VaV

Název: Česká infrastruktura pro integrativní strukturní biologii

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, CIISB - Česká infrastruktura pro integrativní strukturní biologii

MUNI/A/1790/2024, interní kód MU

Název: Patofyziologie běžných komplexních nemocí: od molekuly k organizmu

Investor: Masarykova univerzita, Patofyziologie běžných komplexních nemocí: od molekuly k organizmu

Citovat

BIRGUSOVA, Eliska; Jiří NAVRÁTIL; Eliska DOSTALOVA; Amir M ASHRAFI; Zuzana BYTESNIKOVA; Jan PŘIBYL a Lukas RICHTERA. TP53 detection based on electrochemical genosensors with different types of gold nanoparticles. Microchemical Journal. AMSTERDAM: Elsevier, 2025, roč. 209, February 2025, s. 1-10. ISSN 0026-265X. Dostupné z: https://doi.org/10.1016/j.microc.2025.112856.

@article{2477122,
   author = {Birgusova, Eliska and Navrátil, Jiří and Dostalova, Eliska and Ashrafi, Amir M and Bytesnikova, Zuzana and Přibyl, Jan and Richtera, Lukas},
   article_location = {AMSTERDAM},
   article_number = {February 2025},
   doi = {https://doi.org/10.1016/j.microc.2025.112856},
   keywords = {Electrochemical genosensor; Gold-based nanomaterials; Diagnostic tool; TP53; P53},
   language = {eng},
   issn = {0026-265X},
   journal = {Microchemical Journal},
   title = {TP53 detection based on electrochemical genosensors with different types of gold nanoparticles},
   url = {https://www.sciencedirect.com/science/article/pii/S0026265X25002103},
   volume = {209},
   year = {2025}
}

TY  - JOUR
ID  - 2477122
AU  - Birgusova, Eliska - Navrátil, Jiří - Dostalova, Eliska - Ashrafi, Amir M - Bytesnikova, Zuzana - Přibyl, Jan - Richtera, Lukas
PY  - 2025
TI  - TP53 detection based on electrochemical genosensors with different types of gold nanoparticles
JF  - Microchemical Journal
VL  - 209
IS  - February 2025
SP  - 1-10
EP  - 1-10
PB  - Elsevier
SN  - 0026265X
KW  - Electrochemical genosensor
KW  - Gold-based nanomaterials
KW  - Diagnostic tool
KW  - TP53
KW  - P53
UR  - https://www.sciencedirect.com/science/article/pii/S0026265X25002103
N2  - The TP53 gene, a critical tumour suppressor, is mutated in about half of human cancer cases. Current detection methods are costly and time-consuming. We introduce a label-free electrochemical genosensor for rapid and sensitive TP53 gene detection. Electrode modification with various Au-based nanomaterials, including AuNPs, 3D-mu flowers, and Au-rGO, was investigated. The developed genosensors showed high selectivity and a low LOD of 0.16 pM. Precision was confirmed with RSD% values of 4.63 % (repeatability) and 4.74 % (reproducibility). Above all, we focused on individual materials and their properties when measuring the TP53 gene for future applications. The genosensor operates effectively within the physiological concentration range of 50 to 100 nM, offering a promising tool for cancer diagnostics.
ER  -

BIRGUSOVA, Eliska; Jiří NAVRÁTIL; Eliska DOSTALOVA; Amir M ASHRAFI; Zuzana BYTESNIKOVA; Jan PŘIBYL a Lukas RICHTERA. TP53 detection based on electrochemical genosensors with different types of gold nanoparticles. \textit{Microchemical Journal}. AMSTERDAM: Elsevier, 2025, roč.~209, February 2025, s.~1-10. ISSN~0026-265X. Dostupné z: https://doi.org/10.1016/j.microc.2025.112856.

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