Heat stress impairs centromere structure and segregation of
meiotic chromosomes in Arabidopsis

J 2024

Heat stress impairs centromere structure and segregation of meiotic chromosomes in Arabidopsis

CRHÁK KHAITOVÁ, Lucie; Pavlína MIKULKOVÁ; Jana PEČINKOVÁ; Manikandan KALIDASS; Stefan HECKMANN et. al.

Základní údaje

Originální název

Heat stress impairs centromere structure and segregation of meiotic chromosomes in Arabidopsis

Autoři

CRHÁK KHAITOVÁ, Lucie; Pavlína MIKULKOVÁ ; Jana PEČINKOVÁ ; Manikandan KALIDASS; Stefan HECKMANN; Inna LERMONTOVA a Karel ŘÍHA

Vydání

elife, CAMBRIDGE, ELIFE SCIENCES PUBLICATIONS LTD, 2024, 2050-084X

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10601 Cell biology

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 6.400 v roce 2023

Kód RIV

RIV/00216224:14740/24:00138863

Organizační jednotka

Středoevropský technologický institut

DOI

https://doi.org/10.7554/eLife.90253

UT WoS

001205229000001

Klíčová slova anglicky

meiosis; centremeres; micronuclei; spindle assembly checkpoint; centromeric histone; A. thaliana

Štítky

CF CELLIM, CF PLANT, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 9. 6. 2025 15:29, Mgr. Eva Dubská

Anotace

V originále

Heat stress is a major threat to global crop production, and understanding its impact on plant fertility is crucial for developing climate-resilient crops. Despite the known negative effects of heat stress on plant reproduction, the underlying molecular mechanisms remain poorly understood. Here, we investigated the impact of elevated temperature on centromere structure and chromosome segregation during meiosis in Arabidopsis thaliana. Consistent with previous studies, heat stress leads to a decline in fertility and micronuclei formation in pollen mother cells. Our results reveal that elevated temperature causes a decrease in the amount of centromeric histone and the kinetochore protein BMF1 at meiotic centromeres with increasing temperature. Furthermore, we show that heat stress increases the duration of meiotic divisions and prolongs the activity of the spindle assembly checkpoint during meiosis I, indicating an impaired efficiency of the kinetochore attachments to spindle microtubules. Our analysis of mutants with reduced levels of centromeric histone suggests that weakened centromeres sensitize plants to elevated temperature, resulting in meiotic defects and reduced fertility even at moderate temperatures. These results indicate that the structure and functionality of meiotic centromeres in Arabidopsis are highly sensitive to heat stress, and suggest that centromeres and kinetochores may represent a critical bottleneck in plant adaptation to increasing temperatures.

Návaznosti

GC21-25163J, projekt VaV

Název: Funkce a dynamika telomér v rostlinné meióze

Investor: Grantová agentura ČR, Funkce a dynamika telomér v rostlinné meióze, Německo/DFG

90129, velká výzkumná infrastruktura

Název: Czech-BioImaging II

Citovat

CRHÁK KHAITOVÁ, Lucie; Pavlína MIKULKOVÁ; Jana PEČINKOVÁ; Manikandan KALIDASS; Stefan HECKMANN; Inna LERMONTOVA a Karel ŘÍHA. Heat stress impairs centromere structure and segregation of meiotic chromosomes in Arabidopsis. elife. CAMBRIDGE: ELIFE SCIENCES PUBLICATIONS LTD, 2024, roč. 12, apr, s. 1-18. ISSN 2050-084X. Dostupné z: https://doi.org/10.7554/eLife.90253.

@article{2477297,
   author = {Crhák Khaitová, Lucie and Mikulková, Pavlína and Pečinková, Jana and Kalidass, Manikandan and Heckmann, Stefan and Lermontova, Inna and Říha, Karel},
   article_location = {CAMBRIDGE},
   article_number = {apr},
   doi = {https://doi.org/10.7554/eLife.90253},
   keywords = {meiosis; centremeres; micronuclei; spindle assembly checkpoint; centromeric histone; A. thaliana},
   language = {eng},
   issn = {2050-084X},
   journal = {elife},
   title = {Heat stress impairs centromere structure and segregation of meiotic chromosomes in Arabidopsis},
   url = {https://elifesciences.org/articles/90253},
   volume = {12},
   year = {2024}
}

TY  - JOUR
ID  - 2477297
AU  - Crhák Khaitová, Lucie - Mikulková, Pavlína - Pečinková, Jana - Kalidass, Manikandan - Heckmann, Stefan - Lermontova, Inna - Říha, Karel
PY  - 2024
TI  - Heat stress impairs centromere structure and segregation of meiotic chromosomes in Arabidopsis
JF  - elife
VL  - 12
IS  - apr
SP  - 1-18
EP  - 1-18
PB  - ELIFE SCIENCES PUBLICATIONS LTD
SN  - 2050084X
KW  - meiosis
KW  - centremeres
KW  - micronuclei
KW  - spindle assembly checkpoint
KW  - centromeric histone
KW  - A. thaliana
UR  - https://elifesciences.org/articles/90253
N2  - Heat stress is a major threat to global crop production, and understanding its impact on plant fertility is crucial for developing climate-resilient crops. Despite the known negative effects of heat stress on plant reproduction, the underlying molecular mechanisms remain poorly understood. Here, we investigated the impact of elevated temperature on centromere structure and chromosome segregation during meiosis in Arabidopsis thaliana. Consistent with previous studies, heat stress leads to a decline in fertility and micronuclei formation in pollen mother cells. Our results reveal that elevated temperature causes a decrease in the amount of centromeric histone and the kinetochore protein BMF1 at meiotic centromeres with increasing temperature. Furthermore, we show that heat stress increases the duration of meiotic divisions and prolongs the activity of the spindle assembly checkpoint during meiosis I, indicating an impaired efficiency of the kinetochore attachments to spindle microtubules. Our analysis of mutants with reduced levels of centromeric histone suggests that weakened centromeres sensitize plants to elevated temperature, resulting in meiotic defects and reduced fertility even at moderate temperatures. These results indicate that the structure and functionality of meiotic centromeres in Arabidopsis are highly sensitive to heat stress, and suggest that centromeres and kinetochores may represent a critical bottleneck in plant adaptation to increasing temperatures.
ER  -

CRHÁK KHAITOVÁ, Lucie; Pavlína MIKULKOVÁ; Jana PEČINKOVÁ; Manikandan KALIDASS; Stefan HECKMANN; Inna LERMONTOVA a Karel ŘÍHA. Heat stress impairs centromere structure and segregation of meiotic chromosomes in Arabidopsis. \textit{elife}. CAMBRIDGE: ELIFE SCIENCES PUBLICATIONS LTD, 2024, roč.~12, apr, s.~1-18. ISSN~2050-084X. Dostupné z: https://doi.org/10.7554/eLife.90253.

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