Chemical Targeting of Rhodol Voltage-Sensitive Dyes to
Dopaminergic Neurons

J 2022

Chemical Targeting of Rhodol Voltage-Sensitive Dyes to Dopaminergic Neurons

FIALA, Tomáš; Eugene V MOSHAROV; Jihang WANG; Adriana M MENDIETA; Se Joon CHOI et al.

Základní údaje

Originální název

Chemical Targeting of Rhodol Voltage-Sensitive Dyes to Dopaminergic Neurons

Autoři

FIALA, Tomáš ; Eugene V MOSHAROV; Jihang WANG; Adriana M MENDIETA; Se Joon CHOI; Eva FIALOVA; Christopher HWU; David SULZER a Dalibor SAMES

Vydání

ACS Chemical Neuroscience, WASHINGTON, American Chemical Society, 2022, 1948-7193

Další údaje

Typ výsledku

Článek v odborném periodiku

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 5.000

Označené pro přenos do RIV

DOI

https://doi.org/10.1021/acschemneuro.1c00862

Klíčová slova anglicky

voltage-sensitive dyes; chemical targeting; cell type-specific; maging; molecular; maging probes

Štítky

ne MU, ne RIV

Změněno: 17. 2. 2025 10:33, Mgr. Pavla Foltynová, Ph.D.

Anotace

V originále

Optical imaging of changes in the membrane potential of living cells can be achieved by means of fluorescent voltage-sensitive dyes (VSDs). A particularly challenging task is to efficiently deliver these highly lipophilic probes to specific neuronal subpopulations in brain tissue. We have tackled this task by designing a solubilizing, hydrophilic polymer platform that carries a high-affinity ligand for a membrane protein marker of interest and a fluorescent VSD. Here, we disclose an improved design of polymer-supported probes for chemical, nongenetic targeting of voltage sensors to axons natively expressing the dopamine transporter in ex vivo mouse brain tissue. We first show that for negatively charged rhodol VSDs functioning on the photoinduced electron transfer principle, poly(ethylene glycol) as a carrier enables targeting with higher selectivity than the polysaccharide dextran in HEK cell culture. In the same experimental setting, we also demonstrate that incorporation of an azetidine ring into the rhodol chromophore substantially increases the brightness and voltage sensitivity of the respective VSD. We show that the superior properties of the optimized sensor are transferable to recording of electrically evoked activity from dopaminergic axons in mouse striatal slices after averaging of multiple trials. Finally, we suggest the next milestones for the field to achieve single-scan recordings with nongenetically targeted VSDs in native brain tissue.

Citovat

FIALA, Tomáš; Eugene V MOSHAROV; Jihang WANG; Adriana M MENDIETA; Se Joon CHOI; Eva FIALOVA; Christopher HWU; David SULZER a Dalibor SAMES. Chemical Targeting of Rhodol Voltage-Sensitive Dyes to Dopaminergic Neurons. ACS Chemical Neuroscience. WASHINGTON: American Chemical Society, 2022, roč. 13, č. 8, s. 1251-1262. ISSN 1948-7193. Dostupné z: https://doi.org/10.1021/acschemneuro.1c00862.

@article{2477819,
   author = {Fiala, Tomáš and Mosharov, Eugene V and Wang, Jihang and Mendieta, Adriana M and Choi, Se Joon and Fialova, Eva and Hwu, Christopher and Sulzer, David and Sames, Dalibor},
   article_location = {WASHINGTON},
   article_number = {8},
   doi = {https://doi.org/10.1021/acschemneuro.1c00862},
   keywords = {voltage-sensitive dyes; chemical targeting; cell type-specific; maging; molecular; maging probes},
   issn = {1948-7193},
   journal = {ACS Chemical Neuroscience},
   note = {nevzniklo na MU},
   title = {Chemical Targeting of Rhodol Voltage-Sensitive Dyes to Dopaminergic Neurons},
   volume = {13},
   year = {2022}
}

TY  - JOUR
ID  - 2477819
AU  - Fiala, Tomáš - Mosharov, Eugene V - Wang, Jihang - Mendieta, Adriana M - Choi, Se Joon - Fialova, Eva - Hwu, Christopher - Sulzer, David - Sames, Dalibor
PY  - 2022
TI  - Chemical Targeting of Rhodol Voltage-Sensitive Dyes to Dopaminergic Neurons
JF  - ACS Chemical Neuroscience
VL  - 13
IS  - 8
SP  - 1251-1262
EP  - 1251-1262
PB  - American Chemical Society
SN  - 19487193
N1  - nevzniklo na MU
KW  - voltage-sensitive dyes
KW  - chemical targeting
KW  - cell type-specific
KW  - maging
KW  - molecular
KW  - maging probes
N2  - Optical imaging of changes in the membrane potential of living cells can be achieved by means of fluorescent voltage-sensitive dyes (VSDs). A particularly challenging task is to efficiently deliver these highly lipophilic probes to specific neuronal subpopulations in brain tissue. We have tackled this task by designing a solubilizing, hydrophilic polymer platform that carries a high-affinity ligand for a membrane protein marker of interest and a fluorescent VSD. Here, we disclose an improved design of polymer-supported probes for chemical, nongenetic targeting of voltage sensors to axons natively expressing the dopamine transporter in ex vivo mouse brain tissue. We first show that for negatively charged rhodol VSDs functioning on the photoinduced electron transfer principle, poly(ethylene glycol) as a carrier enables targeting with higher selectivity than the polysaccharide dextran in HEK cell culture. In the same experimental setting, we also demonstrate that incorporation of an azetidine ring into the rhodol chromophore substantially increases the brightness and voltage sensitivity of the respective VSD. We show that the superior properties of the optimized sensor are transferable to recording of electrically evoked activity from dopaminergic axons in mouse striatal slices after averaging of multiple trials. Finally, we suggest the next milestones for the field to achieve single-scan recordings with nongenetically targeted VSDs in native brain tissue.
ER  -

FIALA, Tomáš; Eugene V MOSHAROV; Jihang WANG; Adriana M MENDIETA; Se Joon CHOI; Eva FIALOVA; Christopher HWU; David SULZER a Dalibor SAMES. Chemical Targeting of Rhodol Voltage-Sensitive Dyes to Dopaminergic Neurons. \textit{ACS Chemical Neuroscience}. WASHINGTON: American Chemical Society, 2022, roč.~13, č.~8, s.~1251-1262. ISSN~1948-7193. Dostupné z: https://doi.org/10.1021/acschemneuro.1c00862.

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