FSP1/S100A4-Expressing Stem/Progenitor Cells Are Essential for
Temporomandibular Joint Growth and Homeostasis.

J 2025

FSP1/S100A4-Expressing Stem/Progenitor Cells Are Essential for Temporomandibular Joint Growth and Homeostasis.

TUWATNAWANIT, Ticha; Wilma WESSMAN; Denisa BELISOVÁ; Zuzana SUMBALOVÁ KOLEDOVÁ; Abigail Saffron TUCKER et al.

Základní údaje

Originální název

FSP1/S100A4-Expressing Stem/Progenitor Cells Are Essential for Temporomandibular Joint Growth and Homeostasis.

Autoři

TUWATNAWANIT, Ticha; Wilma WESSMAN; Denisa BELISOVÁ; Zuzana SUMBALOVÁ KOLEDOVÁ; Abigail Saffron TUCKER a Neal ANTHWAL

Vydání

JOURNAL OF DENTAL RESEARCH, THOUSAND OAK, SAGE PUBLICATIONS INC, 2025, 0022-0345

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10601 Cell biology

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 5.900 v roce 2024

Označené pro přenos do RIV

Ano

Organizační jednotka

Lékařská fakulta

Klíčová slova anglicky

FSP1/S100A4; stem/progenitor cells; temporomandibular joint; tissue growth; joint homeostasis

Štítky

14110517, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 10. 3. 2026 10:52, Mgr. Tereza Miškechová

Anotace

V originále

The temporomandibular joint (TMJ) is one of the most used joints in the body. Defects and wear in the cartilage of the joint, condyle, and fibrocartilage disc lie at the heart of many common TMJ disorders. During postnatal development, the condyle acts as a growth center for the mandible, with cells moving as a conveyor belt away from the top of the condyle as they differentiate. The superficial layers of the condyle have been proposed to contain stem/progenitor populations to allow growth and maintain homeostasis. Here we have focused on the role of fibroblast-specific protein 1 (FSP1; also known as S100a4) as a key fibroblast stem/progenitor marker for the condyle. Lineage tracing with FSP1-Cre;R26RmTmG mice revealed that FSP1-expressing cells were restricted to the superficial fibroblast zone, giving rise to all layers of the condyle over time. The FSP1-expressing cells overlapped with other putative stem cell markers of the condyle, such as Gli1 and scleraxis. BrdU pulse chase experiments highlighted that a subset of FSP1 fibrocartilage was label retaining, suggesting that FSP1 labels a novel stem/progenitor cell population in the condyle. Destruction of FSP1-expressing cells by conditional diphtheria toxin activity in FSP1-Cre;R26RDTA mice resulted in severe TMJ osteoarthritis with loss of the cartilage structure. Lgr5-expressing cells in the superficial layer of the condyle have been shown to create a Wnt inhibitory niche. FSP1 expression postnatally was associated with a reduction in canonical Wnt activity in the condyle. Importantly, constitutive activation of Wnt/β catenin in FSP1-expressing cells led to a downregulation of FSP1 and progressive postnatal loss of TMJ condylar hyaline cartilage due to loss of the superficial stem/progenitor cells. These data demonstrate a novel role for FSP1-expressing cells in the superficial zone in growth and maintenance of the TMJ condylar cartilage and highlight the importance of regulating Wnt activity in this population.

Citovat

TUWATNAWANIT, Ticha; Wilma WESSMAN; Denisa BELISOVÁ; Zuzana SUMBALOVÁ KOLEDOVÁ; Abigail Saffron TUCKER a Neal ANTHWAL. FSP1/S100A4-Expressing Stem/Progenitor Cells Are Essential for Temporomandibular Joint Growth and Homeostasis. JOURNAL OF DENTAL RESEARCH. THOUSAND OAK: SAGE PUBLICATIONS INC, 2025, roč. 104, č. 5, s. 551-560. ISSN 0022-0345. Dostupné z: https://doi.org/10.1177/00220345251313795.

@article{2478237,
   author = {Tuwatnawanit, Ticha and Wessman, Wilma and Belisová, Denisa and Sumbalová Koledová, Zuzana and Tucker, Abigail Saffron and Anthwal, Neal},
   article_location = {THOUSAND OAK},
   article_number = {5},
   doi = {https://doi.org/10.1177/00220345251313795},
   keywords = {FSP1/S100A4; stem/progenitor cells; temporomandibular joint; tissue growth; joint homeostasis},
   language = {eng},
   issn = {0022-0345},
   journal = {JOURNAL OF DENTAL RESEARCH},
   title = {FSP1/S100A4-Expressing Stem/Progenitor Cells Are Essential for Temporomandibular Joint Growth and Homeostasis.},
   url = {https://journals.sagepub.com/doi/10.1177/00220345251313795?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub%20%200pubmed},
   volume = {104},
   year = {2025}
}

TY  - JOUR
ID  - 2478237
AU  - Tuwatnawanit, Ticha - Wessman, Wilma - Belisová, Denisa - Sumbalová Koledová, Zuzana - Tucker, Abigail Saffron - Anthwal, Neal
PY  - 2025
TI  - FSP1/S100A4-Expressing Stem/Progenitor Cells Are Essential for Temporomandibular Joint Growth and Homeostasis.
JF  - JOURNAL OF DENTAL RESEARCH
VL  - 104
IS  - 5
SP  - 551-560
EP  - 551-560
PB  - SAGE PUBLICATIONS INC
SN  - 00220345
KW  - FSP1/S100A4
KW  - stem/progenitor cells
KW  - temporomandibular joint
KW  - tissue growth
KW  - joint homeostasis
UR  - https://journals.sagepub.com/doi/10.1177/00220345251313795?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub%20%200pubmed
N2  - The temporomandibular joint (TMJ) is one of the most used joints in the body. Defects and wear in the cartilage of the joint, condyle, and fibrocartilage disc lie at the heart of many common TMJ disorders. During postnatal development, the condyle acts as a growth center for the mandible, with cells moving as a conveyor belt away from the top of the condyle as they differentiate. The superficial layers of the condyle have been proposed to contain stem/progenitor populations to allow growth and maintain homeostasis. Here we have focused on the role of fibroblast-specific protein 1 (FSP1; also known as S100a4) as a key fibroblast stem/progenitor marker for the condyle. Lineage tracing with FSP1-Cre;R26RmTmG mice revealed that FSP1-expressing cells were restricted to the superficial fibroblast zone, giving rise to all layers of the condyle over time. The FSP1-expressing cells overlapped with other putative stem cell markers of the condyle, such as Gli1 and scleraxis. BrdU pulse chase experiments highlighted that a subset of FSP1 fibrocartilage was label retaining, suggesting that FSP1 labels a novel stem/progenitor cell population in the condyle. Destruction of FSP1-expressing cells by conditional diphtheria toxin activity in FSP1-Cre;R26RDTA mice resulted in severe TMJ osteoarthritis with loss of the cartilage structure. Lgr5-expressing cells in the superficial layer of the condyle have been shown to create a Wnt inhibitory niche. FSP1 expression postnatally was associated with a reduction in canonical Wnt activity in the condyle. Importantly, constitutive activation of Wnt/β catenin in FSP1-expressing cells led to a downregulation of FSP1 and progressive postnatal loss of TMJ condylar hyaline cartilage due to loss of the superficial stem/progenitor cells. These data demonstrate a novel role for FSP1-expressing cells in the superficial zone in growth and maintenance of the TMJ condylar cartilage and highlight the importance of regulating Wnt activity in this population.
ER  -

TUWATNAWANIT, Ticha; Wilma WESSMAN; Denisa BELISOVÁ; Zuzana SUMBALOVÁ KOLEDOVÁ; Abigail Saffron TUCKER a Neal ANTHWAL. FSP1/S100A4-Expressing Stem/Progenitor Cells Are Essential for Temporomandibular Joint Growth and Homeostasis. \textit{JOURNAL OF DENTAL RESEARCH}. THOUSAND OAK: SAGE PUBLICATIONS INC, 2025, roč.~104, č.~5, s.~551-560. ISSN~0022-0345. Dostupné z: https://doi.org/10.1177/00220345251313795.

Přehled o publikaci