The Protective Effect of Serum Antibodies in Preventing
SARS-CoV-2 Virus Entry Into Cardiac Muscle

J 2024

The Protective Effect of Serum Antibodies in Preventing SARS-CoV-2 Virus Entry Into Cardiac Muscle

KESSLER, Markéta; Tomáš VOJTÍŠEK; Tomáš ZEMAN; Jan KRAJSA; Michal SRNÍK et al.

Základní údaje

Originální název

The Protective Effect of Serum Antibodies in Preventing SARS-CoV-2 Virus Entry Into Cardiac Muscle

Autoři

KESSLER, Markéta; Tomáš VOJTÍŠEK; Tomáš ZEMAN; Jan KRAJSA; Michal SRNÍK; Radka DZIEDZINSKÁ a Omar ŠERÝ

Vydání

PHYSIOLOGICAL RESEARCH, CZECH REPUBLIC, ACAD SCIENCES CZECH REPUBLIC, INST PHYSIOLOGY, 2024, 0862-8408

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10607 Virology

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 2.000

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/24:00140025

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

SARS-CoV-2; Antibody; IgG; IgM; Cardiac damage; qPCR; Pneumonia; Pulmonary embolism; Heart failure

Štítky

14110111, 14313050, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 14. 4. 2025 11:56, Mgr. Marie Novosadová Šípková, DiS.

Anotace

V originále

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has been associated with significant cardiovascular complications, including myocardial infection and pulmonary embolism. This study aims to elucidate the relationship between the presence of SARS-CoV-2 RNA in the myocardium of the left ventricle and the levels of IgG and IgM antibodies against the SARS-CoV-2 virus in deceased COVID-19 patients. We conducted a post-mortem examination on 91 individuals who succumbed to COVID-19- related complications. The presence of SARS-CoV-2 RNA in the myocardium of the left ventricle was analyzed reverse transcription real time PCR (RT-qPCR) (EliGene® COVID19 UKV/SAV RT kit, Elisabeth Pharmacon), and antibody levels in serum were analyzed by serological assays (VIDAS SARS-COV-2 IgM and VIDAS SARS-COV-2 IgG II tests, BioMérieux). Of the heart tissue samples, 44 % tested positive for SARS-CoV-2 RNA. Our findings indicate that any detectable level of IgG antibodies against SARS-CoV-2 reduces the risk of viral penetration into the myocardium by more than fourfold. Specifically, individuals with detectable levels of IgG and IgM antibodies exhibited a significantly reduced presence of SARS-CoV-2 RNA in cardiac tissues (p<0.0001 for IgG and p<0.001 for IgM). Notably, all patients who died from pulmonary embolism had elevated levels of IgG antibodies. The study underscores the protective role of IgG and IgM antibodies in preventing SARS-CoV-2 penetration into cardiac tissues. However, high antibody titers were associated with fatal outcomes such as pulmonary embolism, pointing to the intricate balance of immune response in COVID-19 pathology.

Návaznosti

MUNI/A/1582/2023, interní kód MU

Název: Podpora biochemického výzkumu v roce 2024

Investor: Masarykova univerzita, Podpora biochemického výzkumu v roce 2024

Citovat

KESSLER, Markéta; Tomáš VOJTÍŠEK; Tomáš ZEMAN; Jan KRAJSA; Michal SRNÍK; Radka DZIEDZINSKÁ a Omar ŠERÝ. The Protective Effect of Serum Antibodies in Preventing SARS-CoV-2 Virus Entry Into Cardiac Muscle. PHYSIOLOGICAL RESEARCH. CZECH REPUBLIC: ACAD SCIENCES CZECH REPUBLIC, INST PHYSIOLOGY, 2024, roč. 73, S3, s. 715-725. ISSN 0862-8408. Dostupné z: https://doi.org/10.33549/physiolres.935475.

@article{2479080,
   author = {Kessler, Markéta and Vojtíšek, Tomáš and Zeman, Tomáš and Krajsa, Jan and Srník, Michal and Dziedzinská, Radka and Šerý, Omar},
   article_location = {CZECH REPUBLIC},
   article_number = {S3},
   doi = {https://doi.org/10.33549/physiolres.935475},
   keywords = {SARS-CoV-2; Antibody; IgG; IgM; Cardiac damage; qPCR; Pneumonia; Pulmonary embolism; Heart failure},
   language = {eng},
   issn = {0862-8408},
   journal = {PHYSIOLOGICAL RESEARCH},
   title = {The Protective Effect of Serum Antibodies in Preventing SARS-CoV-2 Virus Entry Into Cardiac Muscle},
   url = {https://www.biomed.cas.cz/physiolres/pdf/73/73_S715.pdf},
   volume = {73},
   year = {2024}
}

TY  - JOUR
ID  - 2479080
AU  - Kessler, Markéta - Vojtíšek, Tomáš - Zeman, Tomáš - Krajsa, Jan - Srník, Michal - Dziedzinská, Radka - Šerý, Omar
PY  - 2024
TI  - The Protective Effect of Serum Antibodies in Preventing SARS-CoV-2 Virus Entry Into Cardiac Muscle
JF  - PHYSIOLOGICAL RESEARCH
VL  - 73
IS  - S3
SP  - 715-725
EP  - 715-725
PB  - ACAD SCIENCES CZECH REPUBLIC, INST PHYSIOLOGY
SN  - 08628408
KW  - SARS-CoV-2
KW  - Antibody
KW  - IgG
KW  - IgM
KW  - Cardiac damage
KW  - qPCR
KW  - Pneumonia
KW  - Pulmonary embolism
KW  - Heart failure
UR  - https://www.biomed.cas.cz/physiolres/pdf/73/73_S715.pdf
N2  - Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has been associated with significant cardiovascular complications, including myocardial infection and pulmonary embolism. This study aims to elucidate the relationship between the presence of SARS-CoV-2 RNA in the myocardium of the left ventricle and the levels of IgG and IgM antibodies against the SARS-CoV-2 virus in deceased COVID-19 patients. We conducted a post-mortem examination on 91 individuals who succumbed to COVID-19- related complications. The presence of SARS-CoV-2 RNA in the myocardium of the left ventricle was analyzed reverse transcription real time PCR (RT-qPCR) (EliGene® COVID19 UKV/SAV RT kit, Elisabeth Pharmacon), and antibody levels in serum were analyzed by serological assays (VIDAS SARS-COV-2 IgM and VIDAS SARS-COV-2 IgG II tests, BioMérieux). Of the heart tissue samples, 44 % tested positive for SARS-CoV-2 RNA. Our findings indicate that any detectable level of IgG antibodies against SARS-CoV-2 reduces the risk of viral penetration into the myocardium by more than fourfold. Specifically, individuals with detectable levels of IgG and IgM antibodies exhibited a significantly reduced presence of SARS-CoV-2 RNA in cardiac tissues (p<0.0001 for IgG and p<0.001 for IgM). Notably, all patients who died from pulmonary embolism had elevated levels of IgG antibodies. The study underscores the protective role of IgG and IgM antibodies in preventing SARS-CoV-2 penetration into cardiac tissues. However, high antibody titers were associated with fatal outcomes such as pulmonary embolism, pointing to the intricate balance of immune response in COVID-19 pathology.
ER  -

KESSLER, Markéta; Tomáš VOJTÍŠEK; Tomáš ZEMAN; Jan KRAJSA; Michal SRNÍK; Radka DZIEDZINSKÁ a Omar ŠERÝ. The Protective Effect of Serum Antibodies in Preventing SARS-CoV-2 Virus Entry Into Cardiac Muscle. \textit{PHYSIOLOGICAL RESEARCH}. CZECH REPUBLIC: ACAD SCIENCES CZECH REPUBLIC, INST PHYSIOLOGY, 2024, roč.~73, S3, s.~715-725. ISSN~0862-8408. Dostupné z: https://doi.org/10.33549/physiolres.935475.

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