Detection of thymine stretches in DNA oligonucleotides at the
surface of hanging mercury drop electrode using thymine-mercury
base pair formation

J 2025

Detection of thymine stretches in DNA oligonucleotides at the surface of hanging mercury drop electrode using thymine-mercury base pair formation

HERMANOVÁ, Monika; Zuzana SOLDÁNOVÁ; Marcela HRUŠKOVÁ; Luděk HAVRAN; Miroslav FOJTA et al.

Základní údaje

Originální název

Detection of thymine stretches in DNA oligonucleotides at the surface of hanging mercury drop electrode using thymine-mercury base pair formation

Autoři

HERMANOVÁ, Monika; Zuzana SOLDÁNOVÁ ; Marcela HRUŠKOVÁ; Luděk HAVRAN a Miroslav FOJTA

Vydání

Electrochimica Acta, Elsevier Ltd, 2025, 0013-4686

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10608 Biochemistry and molecular biology

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 5.600 v roce 2024

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/25:00140685

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

Thymine; Mercury ions; Base pair; Cyclic voltammetry; Hanging mercury drop electrode

Štítky

143160, ÚMF

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 5. 3. 2025 08:40, Mgr. Marie Novosadová Šípková, DiS.

Anotace

V originále

Thymine-mercury-thymine base pair is a well described type of metal ion-mediated interaction in DNA, with a frequent use in construction of biosensors for mercury detection. In this work we took advantage of this interaction to study behavior of thymine-containing oligodeoxynucleotide (ODN) layers adsorbed on the surface of hanging mercury drop electrode (HMDE), which served as a source of mercury cations upon applying a mildly positive potential (+0.15 V). We confirmed formation of the thymine-mercury complex at the surface of HMDE by observing a pair of redox peaks specific for mercury in dependence on presence of the thymine stretches in ODNs. Deoxythymidine mono- or triphosphate gave much weaker signals due to the mercury-thymine complexes. We confirmed that the complex formation can cause unwinding of the AT duplex and concluded that this approach can be used to observe desorption of the homothymine ODN from the HMDE surface. Moreover, we were able to detect stretches of as little as three thymines in ODNs derived from different telomeric repeat sequences.

Citovat

HERMANOVÁ, Monika; Zuzana SOLDÁNOVÁ; Marcela HRUŠKOVÁ; Luděk HAVRAN a Miroslav FOJTA. Detection of thymine stretches in DNA oligonucleotides at the surface of hanging mercury drop electrode using thymine-mercury base pair formation. Electrochimica Acta. Elsevier Ltd, 2025, roč. 512, February, s. 1-10. ISSN 0013-4686. Dostupné z: https://doi.org/10.1016/j.electacta.2024.145498.

@article{2479787,
   author = {Hermanová, Monika and Soldánová, Zuzana and Hrušková, Marcela and Havran, Luděk and Fojta, Miroslav},
   article_number = {February},
   doi = {https://doi.org/10.1016/j.electacta.2024.145498},
   keywords = {Thymine; Mercury ions; Base pair; Cyclic voltammetry; Hanging mercury drop electrode},
   language = {eng},
   issn = {0013-4686},
   journal = {Electrochimica Acta},
   title = {Detection of thymine stretches in DNA oligonucleotides at the surface of hanging mercury drop electrode using thymine-mercury base pair formation},
   url = {https://www.sciencedirect.com/science/article/pii/S0013468624017341?via%3Dihub},
   volume = {512},
   year = {2025}
}

TY  - JOUR
ID  - 2479787
AU  - Hermanová, Monika - Soldánová, Zuzana - Hrušková, Marcela - Havran, Luděk - Fojta, Miroslav
PY  - 2025
TI  - Detection of thymine stretches in DNA oligonucleotides at the surface of hanging mercury drop electrode using thymine-mercury base pair formation
JF  - Electrochimica Acta
VL  - 512
IS  - February
SP  - 1-10
EP  - 1-10
PB  - Elsevier Ltd
SN  - 00134686
KW  - Thymine
KW  - Mercury ions
KW  - Base pair
KW  - Cyclic voltammetry
KW  - Hanging mercury drop electrode
UR  - https://www.sciencedirect.com/science/article/pii/S0013468624017341?via%3Dihub
N2  - Thymine-mercury-thymine base pair is a well described type of metal ion-mediated interaction in DNA, with a frequent use in construction of biosensors for mercury detection. In this work we took advantage of this interaction to study behavior of thymine-containing oligodeoxynucleotide (ODN) layers adsorbed on the surface of hanging mercury drop electrode (HMDE), which served as a source of mercury cations upon applying a mildly positive potential (+0.15 V). We confirmed formation of the thymine-mercury complex at the surface of HMDE by observing a pair of redox peaks specific for mercury in dependence on presence of the thymine stretches in ODNs. Deoxythymidine mono- or triphosphate gave much weaker signals due to the mercury-thymine complexes. We confirmed that the complex formation can cause unwinding of the AT duplex and concluded that this approach can be used to observe desorption of the homothymine ODN from the HMDE surface. Moreover, we were able to detect stretches of as little as three thymines in ODNs derived from different telomeric repeat sequences.
ER  -

HERMANOVÁ, Monika; Zuzana SOLDÁNOVÁ; Marcela HRUŠKOVÁ; Luděk HAVRAN a Miroslav FOJTA. Detection of thymine stretches in DNA oligonucleotides at the surface of hanging mercury drop electrode using thymine-mercury base pair formation. \textit{Electrochimica Acta}. Elsevier Ltd, 2025, roč.~512, February, s.~1-10. ISSN~0013-4686. Dostupné z: https://doi.org/10.1016/j.electacta.2024.145498.

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