Immune response in diseased and healthy common carp exposed to
carp edema virus

J 2024

Immune response in diseased and healthy common carp exposed to carp edema virus

MACHÁT, Radek; Lubomir POJEZDAL; Jan GEBAUER; Radek TESARIK; Jitka MOTLOVA et al.

Základní údaje

Originální název

Immune response in diseased and healthy common carp exposed to carp edema virus

Autoři

MACHÁT, Radek; Lubomir POJEZDAL; Jan GEBAUER; Radek TESARIK; Jitka MOTLOVA; Miroslava PALIKOVA a Martin FALDYNA

Vydání

Journal of Fish Diseases, Hoboken, Wiley, 2024, 0140-7775

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

40103 Fishery

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 2.200

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/24:00138938

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

cell-mediated cytotoxicity; complement cascade; mass spectrometry; qRT-PCR; type I interferon pathway

Štítky

14314010, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 27. 2. 2025 14:24, Mgr. Marie Novosadová Šípková, DiS.

Anotace

V originále

The common carp is one of the most economically valuable freshwater fish worldwide and its aquaculture can be severely affected by the koi sleepy disease (KSD)/carp edema virus disease (CEVD). This study explores a natural outbreak of CEVD in a pond containing both clinically healthy and diseased fish of various origins exposed to the virus. We investigated mRNA expression of genes associated with known antiviral immune mechanisms, such as type I interferon signalling and cell-mediated cytotoxicity, and performed a comprehensive protein expression analysis to highlight differences between the two groups in various organs. Significant differences in expression profiles of common carp with and without clinical signs were found to be strongly dependent on the organ from which the sample originated. Components of the complement cascade, including various C3 proteins, exhibited upregulation only in less affected organs, specifically the head kidney and spleen. Other complement proteins such as B/C2 and C9 showed upregulation in the kidney, spleen, and gills but not in the skin. Conversely, lysozymes C and G, were observed to be upregulated in the most affected organs of the skin and gills. This study submits the first description of the immune system related proteome using a mass spectrometry on the samples isolated from fish infected with CEV. It also offers a unique comparison of immune reaction of CEV infected and healthy fish under an infectious pressure.

Citovat

MACHÁT, Radek; Lubomir POJEZDAL; Jan GEBAUER; Radek TESARIK; Jitka MOTLOVA; Miroslava PALIKOVA a Martin FALDYNA. Immune response in diseased and healthy common carp exposed to carp edema virus. Journal of Fish Diseases. Hoboken: Wiley, 2024, roč. 47, č. 11, s. 1-12. ISSN 0140-7775. Dostupné z: https://doi.org/10.1111/jfd.14012.

@article{2480357,
   author = {Machát, Radek and Pojezdal, Lubomir and Gebauer, Jan and Tesarik, Radek and Motlova, Jitka and Palikova, Miroslava and Faldyna, Martin},
   article_location = {Hoboken},
   article_number = {11},
   doi = {https://doi.org/10.1111/jfd.14012},
   keywords = {cell-mediated cytotoxicity; complement cascade; mass spectrometry; qRT-PCR; type I interferon pathway},
   language = {eng},
   issn = {0140-7775},
   journal = {Journal of Fish Diseases},
   title = {Immune response in diseased and healthy common carp exposed to carp edema virus},
   url = {https://doi.org/10.1111/jfd.14012},
   volume = {47},
   year = {2024}
}

TY  - JOUR
ID  - 2480357
AU  - Machát, Radek - Pojezdal, Lubomir - Gebauer, Jan - Tesarik, Radek - Motlova, Jitka - Palikova, Miroslava - Faldyna, Martin
PY  - 2024
TI  - Immune response in diseased and healthy common carp exposed to carp edema virus
JF  - Journal of Fish Diseases
VL  - 47
IS  - 11
SP  - 1-12
EP  - 1-12
PB  - Wiley
SN  - 01407775
KW  - cell-mediated cytotoxicity
KW  - complement cascade
KW  - mass spectrometry
KW  - qRT-PCR
KW  - type I interferon pathway
UR  - https://doi.org/10.1111/jfd.14012
N2  - The common carp is one of the most economically valuable freshwater fish worldwide and its aquaculture can be severely affected by the koi sleepy disease (KSD)/carp edema virus disease (CEVD). This study explores a natural outbreak of CEVD in a pond containing both clinically healthy and diseased fish of various origins exposed to the virus. We investigated mRNA expression of genes associated with known antiviral immune mechanisms, such as type I interferon signalling and cell-mediated cytotoxicity, and performed a comprehensive protein expression analysis to highlight differences between the two groups in various organs. Significant differences in expression profiles of common carp with and without clinical signs were found to be strongly dependent on the organ from which the sample originated. Components of the complement cascade, including various C3 proteins, exhibited upregulation only in less affected organs, specifically the head kidney and spleen. Other complement proteins such as B/C2 and C9 showed upregulation in the kidney, spleen, and gills but not in the skin. Conversely, lysozymes C and G, were observed to be upregulated in the most affected organs of the skin and gills. This study submits the first description of the immune system related proteome using a mass spectrometry on the samples isolated from fish infected with CEV. It also offers a unique comparison of immune reaction of CEV infected and healthy fish under an infectious pressure.
ER  -

MACHÁT, Radek; Lubomir POJEZDAL; Jan GEBAUER; Radek TESARIK; Jitka MOTLOVA; Miroslava PALIKOVA a Martin FALDYNA. Immune response in diseased and healthy common carp exposed to carp edema virus. \textit{Journal of Fish Diseases}. Hoboken: Wiley, 2024, roč.~47, č.~11, s.~1-12. ISSN~0140-7775. Dostupné z: https://doi.org/10.1111/jfd.14012.

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