Enhanced immune response and antimicrobial activity in honey
bees (Apis mellifera) following application of oxalic
acid-glycerine strips

J 2025

Enhanced immune response and antimicrobial activity in honey bees (Apis mellifera) following application of oxalic acid-glycerine strips

PINĎÁKOVÁ, Eliška; Silvie DOSTÁLKOVÁ; Jana JEMELKOVÁ; Jana FÜRSTOVÁ; Jana HURYCHOVÁ et al.

Základní údaje

Originální název

Enhanced immune response and antimicrobial activity in honey bees (Apis mellifera) following application of oxalic acid-glycerine strips

Autoři

PINĎÁKOVÁ, Eliška; Silvie DOSTÁLKOVÁ; Jana JEMELKOVÁ; Jana FÜRSTOVÁ; Jana HURYCHOVÁ; Pavel HYRŠL; Dalibor TITĚRA; Marek PETŘIVALSKÝ; Pavel DOBEŠ a Jiří DANIHLÍK

Vydání

Pesticide Biochemistry and Physiology, Elsevier Inc. 2025, 0048-3575

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

40201 Animal and dairy science

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 4.000 v roce 2024

Organizační jednotka

Přírodovědecká fakulta

DOI

https://doi.org/10.1016/j.pestbp.2025.106353

UT WoS

001440631400001

EID Scopus

2-s2.0-85219009132

Klíčová slova anglicky

Varroa; treatment; Integrated Pest Management; Beekeeping; Oxalic acid; Pharmacodynamics

Štítky

14314010, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 15. 4. 2025 11:32, Mgr. Marie Novosadová Šípková, DiS.

Anotace

V originále

Bee health is influenced by multiple factors, including nutrition, immunity, and parasitic pressures. Since the spread of Varroa destructor, overwintering survival has significantly declined, making it one of the most serious threats to honey bee (Apis mellifera L.) populations worldwide. Natural acaricides, such as oxalic acid (OA), are widely employed for managing Varroa mites; however, their pharmacodynamics, particularly their impacts on honey bee physiology and immunity, remain insufficiently understood. We studied effects of oxalic acid on honey bee workers. The study compared three treatments: flumethrin, OA-glycerine strips (OA-G), and OA trickling (OA-T). Twelve colonies were divided into four groups, with samples collected at five time points (0, 24, 48, 72, and 192 h). Physiological changes were assessed through markers of oxidative stress, longevity, and immune parameters. Exposure to oxalic acid via glycerine strips induced a humoral immune response in adult bees. The antimicrobial activity of hemolymph and levels of antimicrobial peptides (abaecin, apidaecin, defensin, and hymenoptaecin) were elevated between 48 and 192 h after OA-G treatment compared to the control group. In contrast, these parameters were not influenced by OA-T or flumethrin treatment. These findings suggest that OA-G strips activate the honey bee's immune system, providing insights into broader implications of OA use in beekeeping. It is crucial to determine whether the activation of humoral immune systems has positive or negative effects, as well as to develop standardized and reliable treatment protocols that ensure both - health of colonies and their effectiveness in controlling Varroa mite infestations.

Návaznosti

QK1910286, projekt VaV

Název: Efektivní postupy a strategie pro zvládání včelích chorob a udržitelný chov včelstev

Investor: Ministerstvo zemědělství ČR, Efektivní postupy a strategie pro zvládání včelích chorob a udržitelný chov včelstev

QL24010241, projekt VaV

Název: Strategie pro mitigaci vlivu roztoče Varroa destructor a přidružených viróz na zdraví a vitalitu včelstev v měnících se klimatických podmínkách ČR

Investor: Ministerstvo zemědělství ČR, Strategie pro mitigaci vlivu roztoče Varroa destructor a přidružených viróz na zdraví a vitalitu včelstev v měnících se klimatických podmínkách ČR

Citovat

PINĎÁKOVÁ, Eliška; Silvie DOSTÁLKOVÁ; Jana JEMELKOVÁ; Jana FÜRSTOVÁ; Jana HURYCHOVÁ; Pavel HYRŠL; Dalibor TITĚRA; Marek PETŘIVALSKÝ; Pavel DOBEŠ a Jiří DANIHLÍK. Enhanced immune response and antimicrobial activity in honey bees (Apis mellifera) following application of oxalic acid-glycerine strips. Pesticide Biochemistry and Physiology. Elsevier Inc., 2025, roč. 209, April, s. 1-11. ISSN 0048-3575. Dostupné z: https://doi.org/10.1016/j.pestbp.2025.106353.

@article{2481418,
   author = {Pinďáková, Eliška and Dostálková, Silvie and Jemelková, Jana and Fürstová, Jana and Hurychová, Jana and Hyršl, Pavel and Titěra, Dalibor and Petřivalský, Marek and Dobeš, Pavel and Danihlík, Jiří},
   article_number = {April},
   doi = {https://doi.org/10.1016/j.pestbp.2025.106353},
   keywords = {Varroa; treatment; Integrated Pest Management; Beekeeping; Oxalic acid; Pharmacodynamics},
   language = {eng},
   issn = {0048-3575},
   journal = {Pesticide Biochemistry and Physiology},
   title = {Enhanced immune response and antimicrobial activity in honey bees (Apis mellifera) following application of oxalic acid-glycerine strips},
   url = {https://doi.org/10.1016/j.pestbp.2025.106353},
   volume = {209},
   year = {2025}
}

TY  - JOUR
ID  - 2481418
AU  - Pinďáková, Eliška - Dostálková, Silvie - Jemelková, Jana - Fürstová, Jana - Hurychová, Jana - Hyršl, Pavel - Titěra, Dalibor - Petřivalský, Marek - Dobeš, Pavel - Danihlík, Jiří
PY  - 2025
TI  - Enhanced immune response and antimicrobial activity in honey bees (Apis mellifera) following application of oxalic acid-glycerine strips
JF  - Pesticide Biochemistry and Physiology
VL  - 209
IS  - April
SP  - 1-11
EP  - 1-11
PB  - Elsevier Inc.
SN  - 00483575
KW  - Varroa
KW  - treatment
KW  - Integrated Pest Management
KW  - Beekeeping
KW  - Oxalic acid
KW  - Pharmacodynamics
UR  - https://doi.org/10.1016/j.pestbp.2025.106353
N2  - Bee health is influenced by multiple factors, including nutrition, immunity, and parasitic pressures. Since the spread of Varroa destructor, overwintering survival has significantly declined, making it one of the most serious threats to honey bee (Apis mellifera L.) populations worldwide. Natural acaricides, such as oxalic acid (OA), are widely employed for managing Varroa mites; however, their pharmacodynamics, particularly their impacts on honey bee physiology and immunity, remain insufficiently understood. We studied effects of oxalic acid on honey bee workers. The study compared three treatments: flumethrin, OA-glycerine strips (OA-G), and OA trickling (OA-T). Twelve colonies were divided into four groups, with samples collected at five time points (0, 24, 48, 72, and 192 h). Physiological changes were assessed through markers of oxidative stress, longevity, and immune parameters. Exposure to oxalic acid via glycerine strips induced a humoral immune response in adult bees. The antimicrobial activity of hemolymph and levels of antimicrobial peptides (abaecin, apidaecin, defensin, and hymenoptaecin) were elevated between 48 and 192 h after OA-G treatment compared to the control group. In contrast, these parameters were not influenced by OA-T or flumethrin treatment. These findings suggest that OA-G strips activate the honey bee's immune system, providing insights into broader implications of OA use in beekeeping. It is crucial to determine whether the activation of humoral immune systems has positive or negative effects, as well as to develop standardized and reliable treatment protocols that ensure both - health of colonies and their effectiveness in controlling Varroa mite infestations.
ER  -

PINĎÁKOVÁ, Eliška; Silvie DOSTÁLKOVÁ; Jana JEMELKOVÁ; Jana FÜRSTOVÁ; Jana HURYCHOVÁ; Pavel HYRŠL; Dalibor TITĚRA; Marek PETŘIVALSKÝ; Pavel DOBEŠ a Jiří DANIHLÍK. Enhanced immune response and antimicrobial activity in honey bees (Apis mellifera) following application of oxalic acid-glycerine strips. \textit{Pesticide Biochemistry and Physiology}. Elsevier Inc., 2025, roč.~209, April, s.~1-11. ISSN~0048-3575. Dostupné z: https://doi.org/10.1016/j.pestbp.2025.106353.

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