RAD51 separation of function mutation maintenance but preserves
DSB repair

J 2024

RAD51 separation of function mutation maintenance but preserves DSB repair

SON, Mi Young; Ondrej BELÁŇ; Mário ŠPÍREK; Jakub CIBULKA; Fedor NIKULENKOV et. al.

Základní údaje

Originální název

RAD51 separation of function mutation maintenance but preserves DSB repair

Autoři

SON, Mi Young; Ondrej BELÁŇ; Mário ŠPÍREK; Jakub CIBULKA; Fedor NIKULENKOV; You Young KIM; Sunyoung HWANG; Kyungjae MYUNG; Cristina MONTAGNA; Tae Moon KIM; Lumír KREJČÍ a Paul HASTY

Vydání

iScience, Cambridge, Cell Press, 2024, 2589-0042

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10608 Biochemistry and molecular biology

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 4.100

Kód RIV

RIV/00216224:14110/24:00139744

Organizační jednotka

Lékařská fakulta

DOI

https://doi.org/10.1016/j.isci.2024.109524

UT WoS

001222093600001

EID Scopus

2-s2.0-85189497725

Klíčová slova anglicky

Properties of biomolecules; Genetics; Molecular biology; Molecular interaction

Štítky

14110513, 143160, podil, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 17. 3. 2025 08:52, Mgr. Tereza Miškechová

Anotace

V originále

Homologous recombination (HR) protects replication forks (RFs) and repairs DNA double-strand breaks (DSBs). Within HR, BRCA2 regulates RAD51 via two interaction regions: the BRC repeats to form filaments on single-stranded DNA and exon 27 (Ex27) to stabilize the filament. Here, we identified a RAD51 S181P mutant that selectively disrupted the RAD51-Ex27 association while maintaining interaction with BRC repeat and proficiently forming filaments capable of DNA binding and strand invasion. Interestingly, RAD51 S181P was defective for RF protection/restart but proficient for DSB repair. Our data suggest that Ex27-mediated stabilization of RAD51 filaments is required for the protection of RFs, while it seems dispensable for the repair of DSBs.

Návaznosti

GX21-22593X, projekt VaV

Název: Identifikace a charakterizace proteinů zahrnutých v metabolismu G-kvadruplexů a R-smyček a jejich vztah k replikaci DNA

206292/E/17/Z, interní kód MU

Název: Mechanics and execution of homologous recombination - biophysics to the organism

Investor: Wellcome Trust, Mechanics and execution of homologous recombination - biophysics to the organism

Citovat

SON, Mi Young; Ondrej BELÁŇ; Mário ŠPÍREK; Jakub CIBULKA; Fedor NIKULENKOV; You Young KIM; Sunyoung HWANG; Kyungjae MYUNG; Cristina MONTAGNA; Tae Moon KIM; Lumír KREJČÍ a Paul HASTY. RAD51 separation of function mutation maintenance but preserves DSB repair. iScience. Cambridge: Cell Press, 2024, roč. 27, č. 4, s. 1-19. ISSN 2589-0042. Dostupné z: https://doi.org/10.1016/j.isci.2024.109524.

@article{2481577,
   author = {Son, Mi Young and Beláň, Ondrej and Špírek, Mário and Cibulka, Jakub and Nikulenkov, Fedor and Kim, You Young and Hwang, Sunyoung and Myung, Kyungjae and Montagna, Cristina and Kim, Tae Moon and Krejčí, Lumír and Hasty, Paul},
   article_location = {Cambridge},
   article_number = {4},
   doi = {https://doi.org/10.1016/j.isci.2024.109524},
   keywords = {Properties of biomolecules; Genetics; Molecular biology; Molecular interaction},
   language = {eng},
   issn = {2589-0042},
   journal = {iScience},
   title = {RAD51 separation of function mutation maintenance but preserves DSB repair},
   url = {https://doi.org/10.1016/j.isci.2024.109524},
   volume = {27},
   year = {2024}
}

TY  - JOUR
ID  - 2481577
AU  - Son, Mi Young - Beláň, Ondrej - Špírek, Mário - Cibulka, Jakub - Nikulenkov, Fedor - Kim, You Young - Hwang, Sunyoung - Myung, Kyungjae - Montagna, Cristina - Kim, Tae Moon - Krejčí, Lumír - Hasty, Paul
PY  - 2024
TI  - RAD51 separation of function mutation maintenance but preserves DSB repair
JF  - iScience
VL  - 27
IS  - 4
SP  - 1-19
EP  - 1-19
PB  - Cell Press
SN  - 25890042
KW  - Properties of biomolecules
KW  - Genetics
KW  - Molecular biology
KW  - Molecular interaction
UR  - https://doi.org/10.1016/j.isci.2024.109524
N2  - Homologous recombination (HR) protects replication forks (RFs) and repairs DNA double-strand breaks (DSBs). Within HR, BRCA2 regulates RAD51 via two interaction regions: the BRC repeats to form filaments on single-stranded DNA and exon 27 (Ex27) to stabilize the filament. Here, we identified a RAD51 S181P mutant that selectively disrupted the RAD51-Ex27 association while maintaining interaction with BRC repeat and proficiently forming filaments capable of DNA binding and strand invasion. Interestingly, RAD51 S181P was defective for RF protection/restart but proficient for DSB repair. Our data suggest that Ex27-mediated stabilization of RAD51 filaments is required for the protection of RFs, while it seems dispensable for the repair of DSBs.
ER  -

SON, Mi Young; Ondrej BELÁŇ; Mário ŠPÍREK; Jakub CIBULKA; Fedor NIKULENKOV; You Young KIM; Sunyoung HWANG; Kyungjae MYUNG; Cristina MONTAGNA; Tae Moon KIM; Lumír KREJČÍ a Paul HASTY. RAD51 separation of function mutation maintenance but preserves DSB repair. \textit{iScience}. Cambridge: Cell Press, 2024, roč.~27, č.~4, s.~1-19. ISSN~2589-0042. Dostupné z: https://doi.org/10.1016/j.isci.2024.109524.

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