Analysis of Telomerase RNA Structure in Physcomitrium patens
Indicates Functionally Relevant Transitions Between OPEN and
CLOSED Conformations

J 2024

Analysis of Telomerase RNA Structure in Physcomitrium patens Indicates Functionally Relevant Transitions Between OPEN and CLOSED Conformations

BOZDĚCHOVÁ, Lucie; Kateřina HAVLOVÁ; Petr FAJKUS a Jiří FAJKUS

Základní údaje

Originální název

Analysis of Telomerase RNA Structure in Physcomitrium patens Indicates Functionally Relevant Transitions Between OPEN and CLOSED Conformations

Autoři

BOZDĚCHOVÁ, Lucie (203 Česká republika, domácí); Kateřina HAVLOVÁ (203 Česká republika, domácí); Petr FAJKUS (203 Česká republika, domácí) a Jiří FAJKUS (203 Česká republika, garant, domácí)

Vydání

Journal of Molecular Biology, LONDON, ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD, 2024, 0022-2836

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10608 Biochemistry and molecular biology

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 4.700 v roce 2023

Kód RIV

RIV/00216224:14740/24:00138984

Organizační jednotka

Středoevropský technologický institut

DOI

http://dx.doi.org/10.1016/j.jmb.2023.168417

UT WoS

001154443600001

EID Scopus

2-s2.0-85181826152

Klíčová slova anglicky

telomerase RNA; RNA secondary structure; single molecule analysis; DMS-Map Seq; SHAPE-Map Seq

Štítky

CF GEN, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 3. 6. 2025 12:43, Mgr. Eva Dubská

Anotace

V originále

Telomerase RNA (TR) conformation determines its function as a template for telomere synthesis and as a scaffold for the assembly of the telomerase nucleoprotein complex. Experimental analyses of TR secondary structure using DMS-Map Seq and SHAPE-Map Seq techniques show its CLOSED conformation as the consensus structure where the template region cannot perform its function. Our data show that the apparent discrepancy between experimental results and predicted TR functional conformation, mostly ignored in published studies, can be explained using data analysis based on single-molecule structure prediction from individual sequencing reads by the recently established DaVinci method. This method results in several clusters of secondary structures reflecting the structural dynamics of TR, possibly related to its multiple functional states. Interestingly, the presumed active (OPEN) conformation of TR corresponds to a minor fraction of TR under in vivo conditions. Therefore, structural polymorphism and dynamic TR transitions between CLOSED and OPEN conformations may be involved in telomerase activity regulation as a switch that functions independently of total TR transcript levels. (c) 2023 Elsevier Ltd. All rights reserved.

Návaznosti

EH22_008/0004581, projekt VaV

Název: Nové poznatky pro plodiny nové generace

GX20-01331X, projekt VaV

Název: Biogeneze a evoluce telomerázy u rostlin

Investor: Grantová agentura ČR, Biogenesis and evolution of telomerase in plants

MUNI/R/1076/2020, interní kód MU

Název: Structural and functional analysis of plant telomerase RNA

Investor: Masarykova univerzita, Structural and functional analysis of plant telomerase RNA, CAREER RESTART

90254, velká výzkumná infrastruktura

Název: e-INFRA CZ II

Citovat

BOZDĚCHOVÁ, Lucie; Kateřina HAVLOVÁ; Petr FAJKUS a Jiří FAJKUS. Analysis of Telomerase RNA Structure in Physcomitrium patens Indicates Functionally Relevant Transitions Between OPEN and CLOSED Conformations. Journal of Molecular Biology. LONDON: ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD, 2024, roč. 436, č. 4, s. 1-12. ISSN 0022-2836. Dostupné z: https://dx.doi.org/10.1016/j.jmb.2023.168417.

@article{2481646,
   author = {Bozděchová, Lucie and Havlová, Kateřina and Fajkus, Petr and Fajkus, Jiří},
   article_location = {LONDON},
   article_number = {4},
   doi = {http://dx.doi.org/10.1016/j.jmb.2023.168417},
   keywords = {telomerase RNA; RNA secondary structure; single molecule analysis; DMS-Map Seq; SHAPE-Map Seq},
   language = {eng},
   issn = {0022-2836},
   journal = {Journal of Molecular Biology},
   title = {Analysis of Telomerase RNA Structure in Physcomitrium patens Indicates Functionally Relevant Transitions Between OPEN and CLOSED Conformations},
   url = {https://www.sciencedirect.com/science/article/pii/S002228362300534X?via%3Dihub},
   volume = {436},
   year = {2024}
}

TY  - JOUR
ID  - 2481646
AU  - Bozděchová, Lucie - Havlová, Kateřina - Fajkus, Petr - Fajkus, Jiří
PY  - 2024
TI  - Analysis of Telomerase RNA Structure in Physcomitrium patens Indicates Functionally Relevant Transitions Between OPEN and CLOSED Conformations
JF  - Journal of Molecular Biology
VL  - 436
IS  - 4
SP  - 1-12
EP  - 1-12
PB  - ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
SN  - 00222836
KW  - telomerase RNA
KW  - RNA secondary structure
KW  - single molecule analysis
KW  - DMS-Map Seq
KW  - SHAPE-Map Seq
UR  - https://www.sciencedirect.com/science/article/pii/S002228362300534X?via%3Dihub
N2  - Telomerase RNA (TR) conformation determines its function as a template for telomere synthesis and as a scaffold for the assembly of the telomerase nucleoprotein complex. Experimental analyses of TR secondary structure using DMS-Map Seq and SHAPE-Map Seq techniques show its CLOSED conformation as the consensus structure where the template region cannot perform its function. Our data show that the apparent discrepancy between experimental results and predicted TR functional conformation, mostly ignored in published studies, can be explained using data analysis based on single-molecule structure prediction from individual sequencing reads by the recently established DaVinci method. This method results in several clusters of secondary structures reflecting the structural dynamics of TR, possibly related to its multiple functional states. Interestingly, the presumed active (OPEN) conformation of TR corresponds to a minor fraction of TR under in vivo conditions. Therefore, structural polymorphism and dynamic TR transitions between CLOSED and OPEN conformations may be involved in telomerase activity regulation as a switch that functions independently of total TR transcript levels. (c) 2023 Elsevier Ltd. All rights reserved.
ER  -

BOZDĚCHOVÁ, Lucie; Kateřina HAVLOVÁ; Petr FAJKUS a Jiří FAJKUS. Analysis of Telomerase RNA Structure in Physcomitrium patens Indicates Functionally Relevant Transitions Between OPEN and CLOSED Conformations. \textit{Journal of Molecular Biology}. LONDON: ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD, 2024, roč.~436, č.~4, s.~1-12. ISSN~0022-2836. Dostupné z: https://dx.doi.org/10.1016/j.jmb.2023.168417.

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