Earthworm and enchytraeid indicator taxa of different land-use
types identified using soil DNA metabarcoding

J 2025

Earthworm and enchytraeid indicator taxa of different land-use types identified using soil DNA metabarcoding

CUARTERO, Jessica; Maria J I BRIONES; Basil M RAST; Beat STIERLI; Claudia MAURER-TROXLER et al.

Základní údaje

Originální název

Earthworm and enchytraeid indicator taxa of different land-use types identified using soil DNA metabarcoding

Autoři

CUARTERO, Jessica; Maria J I BRIONES; Basil M RAST; Beat STIERLI; Claudia MAURER-TROXLER; Anna-Sofia HUG; Franco WIDMER; Jiří SCHLAGHAMERSKÝ a Beat FREY

Vydání

Applied Soil Ecology, AMSTERDAM, NETHERLANDS, ELSEVIER SCIENCE BV, 2025, 0929-1393

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

40104 Soil science

Stát vydavatele

Nizozemské království

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 5.000 v roce 2024

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/25:00140770

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

DNA metabarcoding; Earthworms; Enchytraeids; Indicators; Land use

Štítky

14314020, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 5. 3. 2025 10:36, Mgr. Lucie Jarošová, DiS.

Anotace

V originále

Earthworms and enchytraeids play an important role in biogeochemical cycles and are good indicators of soil fertility. However, assessing their assemblages is difficult, mainly because the methods to identify them require expert knowledge, which becomes a technical challenge when surveying large areas. Soil DNA metabarcoding is a promising method that enables the identification of individual species directly from a bulk composite sample in large field experiments. Here, we investigated in parallel both earthworm (family Lumbricidae) and enchytraeid (family Enchytraeidae) assemblages in three land-use types (arable land, grassland, forest) across 29 Swiss Soil Monitoring Network (NABO) sites, using high-throughput amplicon sequencing of marker genes. For both earthworms and enchytraeids, alpha-diversity was higher in grasslands than in arable land and forests, and it was significantly affected by soil physico-chemical, climate and biological properties, especially pH and climate properties. In addition, we found negative correlations between earthworms alpha-diversity and soil total carbon (TC) content and the soil carbon to nitrogen ratio. Using the DNA metabarcoding, we observed sequences of Aporrectodea nocturna in soils with low pH, while other Aporrectodea species occurred in soils with high pH. We identified Bimastos rubidus in soils with low pH but higher TC, total nitrogen, organic C, and low silt content, while the enchytraeids Cognettia sphagnetorum and Cernosvitoviella atrata occurred in forest soils with high water and organic matter contents. We identified some indicator taxa for the different land-use types, for grassland: Aporrectodea icterica, Lumbricus rubellus, Marionina communis, Fridericia bisetosa and Fridericia connata; arable land: Allolobophora chlorotica, Enchytraeus dichaetus, Achaeta iberica, Prodtodrilus antipae and Fridericia tuberosa; and for forest: Octolasion cyaneum, Octolasion lacteum and Cognettia chlorophila. Although these indicator taxa are unlikely to provide information about the effect of land-use change on soil biodiversity at large spatial scales, these species do drive assemblage differences between land-use types. Soil DNA metabarcoding could therefore assist land managers in monitoring soil biodiversity and quality.

Citovat

CUARTERO, Jessica; Maria J I BRIONES; Basil M RAST; Beat STIERLI; Claudia MAURER-TROXLER; Anna-Sofia HUG; Franco WIDMER; Jiří SCHLAGHAMERSKÝ a Beat FREY. Earthworm and enchytraeid indicator taxa of different land-use types identified using soil DNA metabarcoding. Applied Soil Ecology. AMSTERDAM, NETHERLANDS: ELSEVIER SCIENCE BV, 2025, roč. 206, February, s. "105891", 16 s. ISSN 0929-1393. Dostupné z: https://doi.org/10.1016/j.apsoil.2025.105891.

@article{2481789,
   author = {Cuartero, Jessica and Briones, Maria J I and Rast, Basil M and Stierli, Beat and MaurerandTroxler, Claudia and Hug, AnnaandSofia and Widmer, Franco and Schlaghamerský, Jiří and Frey, Beat},
   article_location = {AMSTERDAM, NETHERLANDS},
   article_number = {February},
   doi = {https://doi.org/10.1016/j.apsoil.2025.105891},
   keywords = {DNA metabarcoding; Earthworms; Enchytraeids; Indicators; Land use},
   language = {eng},
   issn = {0929-1393},
   journal = {Applied Soil Ecology},
   title = {Earthworm and enchytraeid indicator taxa of different land-use types identified using soil DNA metabarcoding},
   url = {https://doi.org/10.1016/j.apsoil.2025.105891},
   volume = {206},
   year = {2025}
}

TY  - JOUR
ID  - 2481789
AU  - Cuartero, Jessica - Briones, Maria J I - Rast, Basil M - Stierli, Beat - Maurer-Troxler, Claudia - Hug, Anna-Sofia - Widmer, Franco - Schlaghamerský, Jiří - Frey, Beat
PY  - 2025
TI  - Earthworm and enchytraeid indicator taxa of different land-use types identified using soil DNA metabarcoding
JF  - Applied Soil Ecology
VL  - 206
IS  - February
SP  - "105891"
EP  - "105891"
PB  - ELSEVIER SCIENCE BV
SN  - 09291393
KW  - DNA metabarcoding
KW  - Earthworms
KW  - Enchytraeids
KW  - Indicators
KW  - Land use
UR  - https://doi.org/10.1016/j.apsoil.2025.105891
N2  - Earthworms and enchytraeids play an important role in biogeochemical cycles and are good indicators of soil fertility. However, assessing their assemblages is difficult, mainly because the methods to identify them require expert knowledge, which becomes a technical challenge when surveying large areas. Soil DNA metabarcoding is a promising method that enables the identification of individual species directly from a bulk composite sample in large field experiments. Here, we investigated in parallel both earthworm (family Lumbricidae) and enchytraeid (family Enchytraeidae) assemblages in three land-use types (arable land, grassland, forest) across 29 Swiss Soil Monitoring Network (NABO) sites, using high-throughput amplicon sequencing of marker genes. For both earthworms and enchytraeids, alpha-diversity was higher in grasslands than in arable land and forests, and it was significantly affected by soil physico-chemical, climate and biological properties, especially pH and climate properties. In addition, we found negative correlations between earthworms alpha-diversity and soil total carbon (TC) content and the soil carbon to nitrogen ratio. Using the DNA metabarcoding, we observed sequences of Aporrectodea nocturna in soils with low pH, while other Aporrectodea species occurred in soils with high pH. We identified Bimastos rubidus in soils with low pH but higher TC, total nitrogen, organic C, and low silt content, while the enchytraeids Cognettia sphagnetorum and Cernosvitoviella atrata occurred in forest soils with high water and organic matter contents. We identified some indicator taxa for the different land-use types, for grassland: Aporrectodea icterica, Lumbricus rubellus, Marionina communis, Fridericia bisetosa and Fridericia connata; arable land: Allolobophora chlorotica, Enchytraeus dichaetus, Achaeta iberica, Prodtodrilus antipae and Fridericia tuberosa; and for forest: Octolasion cyaneum, Octolasion lacteum and Cognettia chlorophila. Although these indicator taxa are unlikely to provide information about the effect of land-use change on soil biodiversity at large spatial scales, these species do drive assemblage differences between land-use types. Soil DNA metabarcoding could therefore assist land managers in monitoring soil biodiversity and quality.
ER  -

CUARTERO, Jessica; Maria J I BRIONES; Basil M RAST; Beat STIERLI; Claudia MAURER-TROXLER; Anna-Sofia HUG; Franco WIDMER; Jiří SCHLAGHAMERSKÝ a Beat FREY. Earthworm and enchytraeid indicator taxa of different land-use types identified using soil DNA metabarcoding. \textit{Applied Soil Ecology}. AMSTERDAM, NETHERLANDS: ELSEVIER SCIENCE BV, 2025, roč.~206, February, s.~''105891'', 16 s. ISSN~0929-1393. Dostupné z: https://doi.org/10.1016/j.apsoil.2025.105891.

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