Assessment of Agrimonia eupatoria L. and Lipophosphonoxin
(DR-6180) Combination for Wound Repair: Bridging the Gap
Between Phytomedicine and Organic Chemistry

J 2024

Assessment of Agrimonia eupatoria L. and Lipophosphonoxin (DR-6180) Combination for Wound Repair: Bridging the Gap Between Phytomedicine and Organic Chemistry

KAŇUCHOVÁ, Miriam; Veronika BRINDZA LACHOVÁ; Kateřina BOGDANOVÁ; Jana SABOVÁ; Petra BONOVÁ et al.

Základní údaje

Originální název

Assessment of Agrimonia eupatoria L. and Lipophosphonoxin (DR-6180) Combination for Wound Repair: Bridging the Gap Between Phytomedicine and Organic Chemistry

Autoři

Vydání

Biomolecules, Basel, Switzerland, MDPI AG, 2024, 2218-273X

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10608 Biochemistry and molecular biology

Stát vydavatele

Švýcarsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 4.800

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14160/24:00139010

Organizační jednotka

Farmaceutická fakulta

Klíčová slova anglicky

skin tissue; extracellular matrix; repair; regeneration; phytotherapy

Štítky

rivok, ÚPL

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 6. 3. 2025 12:49, Mgr. Daniela Černá

Anotace

V originále

Agrimonia eupatoria L. (AE) has a rich tradition of use in wound healing improvement across various cultures worldwide. In previous studies, we revealed that Agrimonia eupatoria L. water extract (AE) possesses a rich polyphenolic composition, displaying remarkable antioxidant properties. Our investigations also demonstrated that lipophosphonoxin (LPPO) exhibited antibacterial efficacy in vitro while preserving the proliferation and differentiation of fibroblasts and keratinocytes. Building upon our prior findings, in this study, we intended to examine whether a combination of AE and LPPO could enhance skin wound healing while retaining antibacterial attributes. The antibacterial activity of AE/LPPO against Staphylococcus aureus was evaluated, alongside its effects on fibroblast-to-myofibroblast transition, the formation of extracellular matrix (ECM), and endothelial cells and keratinocyte proliferation/phenotype. We also investigated AE/LPPO's impact on TGF-beta 1 and VEGF-A signaling in keratinocytes/fibroblasts and endothelial cells, respectively. Additionally, wound healing progression in rats was examined through macroscopic observation and histological analysis. Our results indicate that AE/LPPO promotes myofibroblast-like phenotypic changes and augments ECM deposition. Clinically relevant, the AE/LPPO did not disrupt TGF-beta 1 and VEGF-A signaling and accelerated wound closure in rats. Notably, while AE and LPPO individually exhibited antibacterial activity, their combination did not lead to synergism, rather decreasing antibacterial activity, warranting further examination. These findings underscore substantial wound healing improvement facilitated by AE/LPPO, requiring further exploration in animal models closer to human physiology.

Návaznosti

LX22NPO5103, projekt VaV

Název: Národní institut virologie a bakteriologie (Akronym: NIVB)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Národní institut virologie a bakteriologie, 5.1 EXCELES

Citovat

KAŇUCHOVÁ, Miriam; Veronika BRINDZA LACHOVÁ; Kateřina BOGDANOVÁ; Jana SABOVÁ; Petra BONOVÁ; Tomáš VASILENKO; Ivan KOVÁČ; Martin NOVOTNÝ; Petra MITRENGOVÁ; Nitjawan SAHATSAPAN; Matúš COMA; Emil ŠVAJDLENKA; Milan KOLÁŘ; Peter BOHUŠ; Pavel MUČAJI; Robert ZAJÍČEK; Dominik REJMAN a Peter GÁL. Assessment of Agrimonia eupatoria L. and Lipophosphonoxin (DR-6180) Combination for Wound Repair: Bridging the Gap Between Phytomedicine and Organic Chemistry. Biomolecules. Basel, Switzerland: MDPI AG, 2024, roč. 14, č. 12, s. 1-21. ISSN 2218-273X. Dostupné z: https://doi.org/10.3390/biom14121590.

@article{2481981,
   author = {Kaňuchová, Miriam and Brindza Lachová, Veronika and Bogdanová, Kateřina and Sabová, Jana and Bonová, Petra and Vasilenko, Tomáš and Kováč, Ivan and Novotný, Martin and Mitrengová, Petra and Sahatsapan, Nitjawan and Coma, Matúš and Švajdlenka, Emil and Kolář, Milan and Bohuš, Peter and Mučaji, Pavel and Zajíček, Robert and Rejman, Dominik and Gál, Peter},
   article_location = {Basel, Switzerland},
   article_number = {12},
   doi = {https://doi.org/10.3390/biom14121590},
   keywords = {skin tissue; extracellular matrix; repair; regeneration; phytotherapy},
   language = {eng},
   issn = {2218-273X},
   journal = {Biomolecules},
   title = {Assessment of Agrimonia eupatoria L. and Lipophosphonoxin (DR-6180) Combination for Wound Repair: Bridging the Gap Between Phytomedicine and Organic Chemistry},
   url = {https://www.mdpi.com/2218-273X/14/12/1590},
   volume = {14},
   year = {2024}
}

TY  - JOUR
ID  - 2481981
AU  - Kaňuchová, Miriam - Brindza Lachová, Veronika - Bogdanová, Kateřina - Sabová, Jana - Bonová, Petra - Vasilenko, Tomáš - Kováč, Ivan - Novotný, Martin - Mitrengová, Petra - Sahatsapan, Nitjawan - Coma, Matúš - Švajdlenka, Emil - Kolář, Milan - Bohuš, Peter - Mučaji, Pavel - Zajíček, Robert - Rejman, Dominik - Gál, Peter
PY  - 2024
TI  - Assessment of Agrimonia eupatoria L. and Lipophosphonoxin (DR-6180) Combination for Wound Repair: Bridging the Gap Between Phytomedicine and Organic Chemistry
JF  - Biomolecules
VL  - 14
IS  - 12
SP  - 1-21
EP  - 1-21
PB  - MDPI AG
SN  - 2218273X
KW  - skin tissue
KW  - extracellular matrix
KW  - repair
KW  - regeneration
KW  - phytotherapy
UR  - https://www.mdpi.com/2218-273X/14/12/1590
N2  - Agrimonia eupatoria L. (AE) has a rich tradition of use in wound healing improvement across various cultures worldwide. In previous studies, we revealed that Agrimonia eupatoria L. water extract (AE) possesses a rich polyphenolic composition, displaying remarkable antioxidant properties. Our investigations also demonstrated that lipophosphonoxin (LPPO) exhibited antibacterial efficacy in vitro while preserving the proliferation and differentiation of fibroblasts and keratinocytes. Building upon our prior findings, in this study, we intended to examine whether a combination of AE and LPPO could enhance skin wound healing while retaining antibacterial attributes. The antibacterial activity of AE/LPPO against Staphylococcus aureus was evaluated, alongside its effects on fibroblast-to-myofibroblast transition, the formation of extracellular matrix (ECM), and endothelial cells and keratinocyte proliferation/phenotype. We also investigated AE/LPPO's impact on TGF-beta 1 and VEGF-A signaling in keratinocytes/fibroblasts and endothelial cells, respectively. Additionally, wound healing progression in rats was examined through macroscopic observation and histological analysis. Our results indicate that AE/LPPO promotes myofibroblast-like phenotypic changes and augments ECM deposition. Clinically relevant, the AE/LPPO did not disrupt TGF-beta 1 and VEGF-A signaling and accelerated wound closure in rats. Notably, while AE and LPPO individually exhibited antibacterial activity, their combination did not lead to synergism, rather decreasing antibacterial activity, warranting further examination. These findings underscore substantial wound healing improvement facilitated by AE/LPPO, requiring further exploration in animal models closer to human physiology.
ER  -

KAŇUCHOVÁ, Miriam; Veronika BRINDZA LACHOVÁ; Kateřina BOGDANOVÁ; Jana SABOVÁ; Petra BONOVÁ; Tomáš VASILENKO; Ivan KOVÁČ; Martin NOVOTNÝ; Petra MITRENGOVÁ; Nitjawan SAHATSAPAN; Matúš COMA; Emil ŠVAJDLENKA; Milan KOLÁŘ; Peter BOHUŠ; Pavel MUČAJI; Robert ZAJÍČEK; Dominik REJMAN a Peter GÁL. Assessment of Agrimonia eupatoria L. and Lipophosphonoxin (DR-6180) Combination for Wound Repair: Bridging the Gap Between Phytomedicine and Organic Chemistry. \textit{Biomolecules}. Basel, Switzerland: MDPI AG, 2024, roč.~14, č.~12, s.~1-21. ISSN~2218-273X. Dostupné z: https://doi.org/10.3390/biom14121590.

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