A New Immortalized Human Lacrimal Gland Cell Line

J 2024

A New Immortalized Human Lacrimal Gland Cell Line

GLEIXNER, Sophie; Ingrid ZAHN; Jana DIETRICH; Swati SINGH; Alice DROBNY et. al.

Základní údaje

Originální název

A New Immortalized Human Lacrimal Gland Cell Line

Autoři

Vydání

Advances in Fuel Cells, BASEL, Elsevier, 2024, 2073-4409

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10601 Cell biology

Stát vydavatele

Švýcarsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 5.200

Kód RIV

RIV/00216224:14740/24:00139025

Organizační jednotka

Středoevropský technologický institut

DOI

https://doi.org/10.3390/cells13070622

UT WoS

001201064200001

EID Scopus

2-s2.0-85190379175

Klíčová slova anglicky

lacrimal gland; epithelial cells; immortalized; cell line; dry eye; tears; tear secretion

Štítky

CF BIOIT, CF GEN, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 3. 6. 2025 13:38, Mgr. Eva Dubská

Anotace

V originále

The lacrimal gland is crucial for maintaining ocular health by producing the aqueous component of the tear film, which hydrates and nourishes the ocular surface. Decreased production of this component results in dry eye disease, a condition affecting over 250 million people worldwide. However, the scarcity of primary human material for studying its underlying mechanisms and the absence of a cell model for human lacrimal gland epithelial cells present significant challenges. Here, we describe the generation of immortalized human lacrimal gland cell lines through the introduction of an SV40 antigen. We successfully isolated and characterized three cell clones from a female lacrimal gland donor, confirming their epithelial identity through genomic and protein analyses, including PCR, RNAseq, immunofluorescence and cultivation in a 3D spheroid model. Our findings represent a significant advancement, providing improved accessibility to investigate the molecular pathogenesis mechanisms of dry eye disease and potential therapeutic interventions. We identified the expression of typical epithelial cell marker genes and demonstrated the cells' capability to form 2D cell sheets and 3D spheroids. This establishment of immortalized human lacrimal gland cells with epithelial characteristics holds promise for future comprehensive studies, contributing to a deeper understanding of dry eye disease and its cellular mechanisms.

Návaznosti

90267, velká výzkumná infrastruktura

Název: NCMG III

Citovat

GLEIXNER, Sophie; Ingrid ZAHN; Jana DIETRICH; Swati SINGH; Alice DROBNY; Yanni SCHNEIDER; Raphael SCHWENDNER; Eileen SOCHER; Nicolas BLAVET; Lars BRAEUER; Antoniu-Oreste GOSTIAN; Matthias BALK; Gundula SCHULZE-TANZIL; Claudia GUENTHER; Friedrich PAULSEN a Philipp ARNOLD. A New Immortalized Human Lacrimal Gland Cell Line. Advances in Fuel Cells. BASEL: Elsevier, 2024, roč. 13, č. 7, s. 1-18. ISSN 2073-4409. Dostupné z: https://doi.org/10.3390/cells13070622.

@article{2482618,
   author = {Gleixner, Sophie and Zahn, Ingrid and Dietrich, Jana and Singh, Swati and Drobny, Alice and Schneider, Yanni and Schwendner, Raphael and Socher, Eileen and Blavet, Nicolas and Braeuer, Lars and Gostian, AntoniuandOreste and Balk, Matthias and SchulzeandTanzil, Gundula and Guenther, Claudia and Paulsen, Friedrich and Arnold, Philipp},
   article_location = {BASEL},
   article_number = {7},
   doi = {https://doi.org/10.3390/cells13070622},
   keywords = {lacrimal gland; epithelial cells; immortalized; cell line; dry eye; tears; tear secretion},
   language = {eng},
   issn = {2073-4409},
   journal = {Advances in Fuel Cells},
   title = {A New Immortalized Human Lacrimal Gland Cell Line},
   url = {https://www.mdpi.com/2073-4409/13/7/622},
   volume = {13},
   year = {2024}
}

TY  - JOUR
ID  - 2482618
AU  - Gleixner, Sophie - Zahn, Ingrid - Dietrich, Jana - Singh, Swati - Drobny, Alice - Schneider, Yanni - Schwendner, Raphael - Socher, Eileen - Blavet, Nicolas - Braeuer, Lars - Gostian, Antoniu-Oreste - Balk, Matthias - Schulze-Tanzil, Gundula - Guenther, Claudia - Paulsen, Friedrich - Arnold, Philipp
PY  - 2024
TI  - A New Immortalized Human Lacrimal Gland Cell Line
JF  - Advances in Fuel Cells
VL  - 13
IS  - 7
SP  - 1-18
EP  - 1-18
PB  - Elsevier
SN  - 20734409
KW  - lacrimal gland
KW  - epithelial cells
KW  - immortalized
KW  - cell line
KW  - dry eye
KW  - tears
KW  - tear secretion
UR  - https://www.mdpi.com/2073-4409/13/7/622
N2  - The lacrimal gland is crucial for maintaining ocular health by producing the aqueous component of the tear film, which hydrates and nourishes the ocular surface. Decreased production of this component results in dry eye disease, a condition affecting over 250 million people worldwide. However, the scarcity of primary human material for studying its underlying mechanisms and the absence of a cell model for human lacrimal gland epithelial cells present significant challenges. Here, we describe the generation of immortalized human lacrimal gland cell lines through the introduction of an SV40 antigen. We successfully isolated and characterized three cell clones from a female lacrimal gland donor, confirming their epithelial identity through genomic and protein analyses, including PCR, RNAseq, immunofluorescence and cultivation in a 3D spheroid model. Our findings represent a significant advancement, providing improved accessibility to investigate the molecular pathogenesis mechanisms of dry eye disease and potential therapeutic interventions. We identified the expression of typical epithelial cell marker genes and demonstrated the cells' capability to form 2D cell sheets and 3D spheroids. This establishment of immortalized human lacrimal gland cells with epithelial characteristics holds promise for future comprehensive studies, contributing to a deeper understanding of dry eye disease and its cellular mechanisms.
ER  -

GLEIXNER, Sophie; Ingrid ZAHN; Jana DIETRICH; Swati SINGH; Alice DROBNY; Yanni SCHNEIDER; Raphael SCHWENDNER; Eileen SOCHER; Nicolas BLAVET; Lars BRAEUER; Antoniu-Oreste GOSTIAN; Matthias BALK; Gundula SCHULZE-TANZIL; Claudia GUENTHER; Friedrich PAULSEN a Philipp ARNOLD. A New Immortalized Human Lacrimal Gland Cell Line. \textit{Advances in Fuel Cells}. BASEL: Elsevier, 2024, roč.~13, č.~7, s.~1-18. ISSN~2073-4409. Dostupné z: https://doi.org/10.3390/cells13070622.

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