Proteomic exploration of potential blood biomarkers in
haemophilic arthropathy

J 2024

Proteomic exploration of potential blood biomarkers in haemophilic arthropathy

KALEBOTA, Natasa; Ruder NOVAK; Stela HRKAC; Porin PERIC; Grgur SALAI et. al.

Základní údaje

Originální název

Proteomic exploration of potential blood biomarkers in haemophilic arthropathy

Autoři

Vydání

Health Science Reports, HOBOKEN, Wiley-Blackwell, 2024, 2398-8835

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30304 Public and environmental health

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 2.100

Kód RIV

RIV/00216224:14740/24:00139027

Organizační jednotka

Středoevropský technologický institut

DOI

https://doi.org/10.1002/hsr2.70046

UT WoS

001319923600001

EID Scopus

2-s2.0-85205251964

Klíčová slova anglicky

arthropathy; biomarkers; haemophilia; pathophysiology; proteomics

Štítky

CF PROT, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 9. 6. 2025 14:18, Mgr. Eva Dubská

Anotace

V originále

Background and Aims: The pathophysiology of haemophilic arthropathy (HA) is complex and largely undefined. Proteomic analyses provide insights into the intricate mechanisms of the HA. Our study aimed to identify differentially expressed proteins in relation to the severity of HA, explore their pathophysiological roles, and evaluate their potential as HA biomarkers. Methods: Our cross-sectional observational study encompassed 30 HA patients and 15 healthy subjects. Plasma samples were pooled into three groups of 15 samples from those with severe haemophilic arthropathy (sHA), mild haemophilic arthropathy (mHA) and healthy controls. Proteomic analysis was performed using liquid chromatography-mass spectrometry. The severity of HA was assessed using the World Federation of Haemophilia Physical Examination Score and ultrasonography following the Haemophilia Early Arthropathy Detection with Ultrasound (HEAD-US) guidelines. Results: A total of 788 proteins were identified, with 97% of the uniquely identified proteins being expressed in all analysed groups. We identified several up and downregulated proteins across the groups that were mainly related to inflammatory and immunity-modulating processes, as well as joint degeneration. We highlighted ten proteins relevant for the development of HA: cathepsin G, endoplasmic reticulum aminopeptidase 2, S100-A9, insulin-like growth factor I, apolipoprotein (a), osteopontin, pregnancy zone protein, cartilage oligomeric matrix protein, CD44, and cadherin-related family member 2. Conclusion: Our analysis identified several proteins that shed further light on the distinctive pathogenesis of HA and could serve for biomarker research. However, these results need to be validated on a larger patient group.

Návaznosti

90242, velká výzkumná infrastruktura

Název: CIISB III

90254, velká výzkumná infrastruktura

Název: e-INFRA CZ II

964997, interní kód MU

Název: Alliance for Life Sciences: From Strategies to Actions in Central and Eastern Europe (Akronym: A4L_ACTIONS)

Investor: Evropská unie, Alliance for Life Sciences: From Strategies to Actions in Central and Eastern Europe, Health, demographic change and wellbeing (Societal Challenges)

Citovat

KALEBOTA, Natasa; Ruder NOVAK; Stela HRKAC; Porin PERIC; Grgur SALAI; Marko MOCIBOB; Marija PRANJIC; Zbyněk ZDRÁHAL; Václav PUSTKA; Nadica Laktasic ZERJAVIC; Milan MILOSEVIC; Marijo VODANOVIC; Silva Zupancic SALEK a Lovorka GRGUREVIC. Proteomic exploration of potential blood biomarkers in haemophilic arthropathy. Health Science Reports. HOBOKEN: Wiley-Blackwell, 2024, roč. 7, č. 9, s. 1-12. ISSN 2398-8835. Dostupné z: https://doi.org/10.1002/hsr2.70046.

@article{2483039,
   author = {Kalebota, Natasa and Novak, Ruder and Hrkac, Stela and Peric, Porin and Salai, Grgur and Mocibob, Marko and Pranjic, Marija and Zdráhal, Zbyněk and Pustka, Václav and Zerjavic, Nadica Laktasic and Milosevic, Milan and Vodanovic, Marijo and Salek, Silva Zupancic and Grgurevic, Lovorka},
   article_location = {HOBOKEN},
   article_number = {9},
   doi = {https://doi.org/10.1002/hsr2.70046},
   keywords = {arthropathy; biomarkers; haemophilia; pathophysiology; proteomics},
   language = {eng},
   issn = {2398-8835},
   journal = {Health Science Reports},
   title = {Proteomic exploration of potential blood biomarkers in haemophilic arthropathy},
   url = {https://onlinelibrary.wiley.com/doi/10.1002/hsr2.70046},
   volume = {7},
   year = {2024}
}

TY  - JOUR
ID  - 2483039
AU  - Kalebota, Natasa - Novak, Ruder - Hrkac, Stela - Peric, Porin - Salai, Grgur - Mocibob, Marko - Pranjic, Marija - Zdráhal, Zbyněk - Pustka, Václav - Zerjavic, Nadica Laktasic - Milosevic, Milan - Vodanovic, Marijo - Salek, Silva Zupancic - Grgurevic, Lovorka
PY  - 2024
TI  - Proteomic exploration of potential blood biomarkers in haemophilic arthropathy
JF  - Health Science Reports
VL  - 7
IS  - 9
SP  - 1-12
EP  - 1-12
PB  - Wiley-Blackwell
SN  - 23988835
KW  - arthropathy
KW  - biomarkers
KW  - haemophilia
KW  - pathophysiology
KW  - proteomics
UR  - https://onlinelibrary.wiley.com/doi/10.1002/hsr2.70046
N2  - Background and Aims: The pathophysiology of haemophilic arthropathy (HA) is complex and largely undefined. Proteomic analyses provide insights into the intricate mechanisms of the HA. Our study aimed to identify differentially expressed proteins in relation to the severity of HA, explore their pathophysiological roles, and evaluate their potential as HA biomarkers. Methods: Our cross-sectional observational study encompassed 30 HA patients and 15 healthy subjects. Plasma samples were pooled into three groups of 15 samples from those with severe haemophilic arthropathy (sHA), mild haemophilic arthropathy (mHA) and healthy controls. Proteomic analysis was performed using liquid chromatography-mass spectrometry. The severity of HA was assessed using the World Federation of Haemophilia Physical Examination Score and ultrasonography following the Haemophilia Early Arthropathy Detection with Ultrasound (HEAD-US) guidelines. Results: A total of 788 proteins were identified, with 97% of the uniquely identified proteins being expressed in all analysed groups. We identified several up and downregulated proteins across the groups that were mainly related to inflammatory and immunity-modulating processes, as well as joint degeneration. We highlighted ten proteins relevant for the development of HA: cathepsin G, endoplasmic reticulum aminopeptidase 2, S100-A9, insulin-like growth factor I, apolipoprotein (a), osteopontin, pregnancy zone protein, cartilage oligomeric matrix protein, CD44, and cadherin-related family member 2. Conclusion: Our analysis identified several proteins that shed further light on the distinctive pathogenesis of HA and could serve for biomarker research. However, these results need to be validated on a larger patient group.
ER  -

KALEBOTA, Natasa; Ruder NOVAK; Stela HRKAC; Porin PERIC; Grgur SALAI; Marko MOCIBOB; Marija PRANJIC; Zbyněk ZDRÁHAL; Václav PUSTKA; Nadica Laktasic ZERJAVIC; Milan MILOSEVIC; Marijo VODANOVIC; Silva Zupancic SALEK a Lovorka GRGUREVIC. Proteomic exploration of potential blood biomarkers in haemophilic arthropathy. \textit{Health Science Reports}. HOBOKEN: Wiley-Blackwell, 2024, roč.~7, č.~9, s.~1-12. ISSN~2398-8835. Dostupné z: https://doi.org/10.1002/hsr2.70046.

Přehled o publikaci