Identification of compounds contributing to glucocorticoid
activity in indoor dust supported by orthogonal fractionation☆

J 2025

Identification of compounds contributing to glucocorticoid activity in indoor dust supported by orthogonal fractionation☆

PINTO VIDAL, Felipe Augusto; Martin KRAUSS; Jiří NOVÁK; Lisa Emily MELYMUK; Werner BRACK et al.

Základní údaje

Originální název

Identification of compounds contributing to glucocorticoid activity in indoor dust supported by orthogonal fractionation☆

Autoři

PINTO VIDAL, Felipe Augusto; Martin KRAUSS; Jiří NOVÁK; Lisa Emily MELYMUK ; Werner BRACK a Klára HILSCHEROVÁ

Vydání

Environmental Pollution, OXFORD (ENGLAND), Elsevier Science, 2025, 0269-7491

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10511 Environmental sciences

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 7.300 v roce 2024

Označené pro přenos do RIV

Ano

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

In vitro; Glucocorticoid activity; Indoor dust; Effect-directed analysis; Orthogonal fractionation; Endocrine disrupting compounds

Štítky

143180, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 15. 2. 2026 10:47, Mgr. Michaela Hylsová, Ph.D.

Anotace

V originále

Indoor dust contains various endocrine-disrupting contaminants, yet the effect drivers of observed glucocorticoid activity are completely unknown. This study conducted an effect-directed analysis using orthogonal fractionation to identify effect drivers of glucocorticoid activity in indoor dust. After the detection of bioactivity using a human cell line stably transfected with a reporter gene, the sample underwent parallel HPLC fractionations with octadecyl, pentafluorophenyl, and aminopropyl columns to obtain orthogonal fractions. The bioassays were utilized to screen the fractions and guide efforts towards prioritization of the bioactive chemicals using targeted and nontargeted analysis with LC-HRMS. The glucocorticoid activity of the identified potential candidates was confirmed by their testing in the same bioassay. To assess their contribution to the detected mixture effects, we calculated their relative potencies. This approach led to the identification of two pharmaceuticals, clobetasol propionate and mometasone furoate, at concentrations ranging from ng to mu g per gram of dust, which together accounted for up to 77% of the observed glucocorticoid activity. This is the first report documenting the effect drivers of glucocorticoid receptor agonism in indoor dust; however, together with previous studies of various environmental samples, it documents that in cases when glucocorticoid receptor-agonistic activity is detected, drugs should be considered as likely relevant contaminants. The discovery of potent drugs in household dust highlights concerns for individuals exposed within domestic environments and emphasizes the need to consider pharmaceuticals as relevant contributors to indoor contamination.

Návaznosti

EF19_073/0016943, projekt VaV

Název: Interní grantová agentura Masarykovy univerzity

GA22-32743S, projekt VaV

Název: Propojení složení chemických látek a mikrobiomu v domácím prachu s environmentálními parametry

Investor: Grantová agentura ČR, Propojení složení chemických látek a mikrobiomu v domácím prachu s environmentálními parametry

90269, velká výzkumná infrastruktura

Název: RECETOX RI II

Citovat

PINTO VIDAL, Felipe Augusto; Martin KRAUSS; Jiří NOVÁK; Lisa Emily MELYMUK; Werner BRACK a Klára HILSCHEROVÁ. Identification of compounds contributing to glucocorticoid activity in indoor dust supported by orthogonal fractionation☆. Environmental Pollution. OXFORD (ENGLAND): Elsevier Science, 2025, roč. 367, February 2025, s. 1-9. ISSN 0269-7491. Dostupné z: https://doi.org/10.1016/j.envpol.2024.125579.

@article{2483240,
   author = {Pinto Vidal, Felipe Augusto and Krauss, Martin and Novák, Jiří and Melymuk, Lisa Emily and Brack, Werner and Hilscherová, Klára},
   article_location = {OXFORD (ENGLAND)},
   article_number = {February 2025},
   doi = {https://doi.org/10.1016/j.envpol.2024.125579},
   keywords = {In vitro; Glucocorticoid activity; Indoor dust; Effect-directed analysis; Orthogonal fractionation; Endocrine disrupting compounds},
   language = {eng},
   issn = {0269-7491},
   journal = {Environmental Pollution},
   title = {Identification of compounds contributing to glucocorticoid activity in indoor dust supported by orthogonal fractionation☆},
   url = {https://www.sciencedirect.com/science/article/pii/S0269749124022966?via%3Dihub},
   volume = {367},
   year = {2025}
}

TY  - JOUR
ID  - 2483240
AU  - Pinto Vidal, Felipe Augusto - Krauss, Martin - Novák, Jiří - Melymuk, Lisa Emily - Brack, Werner - Hilscherová, Klára
PY  - 2025
TI  - Identification of compounds contributing to glucocorticoid activity in indoor dust supported by orthogonal fractionation☆
JF  - Environmental Pollution
VL  - 367
IS  - February 2025
SP  - 1-9
EP  - 1-9
PB  - Elsevier Science
SN  - 02697491
KW  - In vitro
KW  - Glucocorticoid activity
KW  - Indoor dust
KW  - Effect-directed analysis
KW  - Orthogonal fractionation
KW  - Endocrine disrupting compounds
UR  - https://www.sciencedirect.com/science/article/pii/S0269749124022966?via%3Dihub
N2  - Indoor dust contains various endocrine-disrupting contaminants, yet the effect drivers of observed glucocorticoid activity are completely unknown. This study conducted an effect-directed analysis using orthogonal fractionation to identify effect drivers of glucocorticoid activity in indoor dust. After the detection of bioactivity using a human cell line stably transfected with a reporter gene, the sample underwent parallel HPLC fractionations with octadecyl, pentafluorophenyl, and aminopropyl columns to obtain orthogonal fractions. The bioassays were utilized to screen the fractions and guide efforts towards prioritization of the bioactive chemicals using targeted and nontargeted analysis with LC-HRMS. The glucocorticoid activity of the identified potential candidates was confirmed by their testing in the same bioassay. To assess their contribution to the detected mixture effects, we calculated their relative potencies. This approach led to the identification of two pharmaceuticals, clobetasol propionate and mometasone furoate, at concentrations ranging from ng to mu g per gram of dust, which together accounted for up to 77% of the observed glucocorticoid activity. This is the first report documenting the effect drivers of glucocorticoid receptor agonism in indoor dust; however, together with previous studies of various environmental samples, it documents that in cases when glucocorticoid receptor-agonistic activity is detected, drugs should be considered as likely relevant contaminants. The discovery of potent drugs in household dust highlights concerns for individuals exposed within domestic environments and emphasizes the need to consider pharmaceuticals as relevant contributors to indoor contamination.
ER  -

PINTO VIDAL, Felipe Augusto; Martin KRAUSS; Jiří NOVÁK; Lisa Emily MELYMUK; Werner BRACK a Klára HILSCHEROVÁ. Identification of compounds contributing to glucocorticoid activity in indoor dust supported by orthogonal fractionation☆. \textit{Environmental Pollution}. OXFORD (ENGLAND): Elsevier Science, 2025, roč.~367, February 2025, s.~1-9. ISSN~0269-7491. Dostupné z: https://doi.org/10.1016/j.envpol.2024.125579.

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