Advancing microplastic detection in zebrafish with micro
computed tomography: A novel approach to revealing microplastic
distribution in organisms

J 2025

Advancing microplastic detection in zebrafish with micro computed tomography: A novel approach to revealing microplastic distribution in organisms

PAROBKOVA, Viktoria; Lukas MALECEK; Marek ZEMEK; Gabriela KALCIKOVA; Michaela VYKYPĚLOVÁ et al.

Základní údaje

Originální název

Advancing microplastic detection in zebrafish with micro computed tomography: A novel approach to revealing microplastic distribution in organisms

Autoři

PAROBKOVA, Viktoria; Lukas MALECEK; Marek ZEMEK; Gabriela KALCIKOVA; Michaela VYKYPĚLOVÁ ; Marcela BUCHTOVÁ; Ondřej ADAMOVSKÝ; Tomas ZIKMUND a Jozef KAISER

Vydání

Journal of Hazardous Materials, Amsterdam, Elsevier Science BV. 2025, 0304-3894

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10511 Environmental sciences

Stát vydavatele

Nizozemské království

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 11.300 v roce 2024

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/25:00140802

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

Microplastics; X-ray Computed Tomography; Environment; Plastic Pollution; Imaging

Štítky

14314010, 143180, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 12. 3. 2025 09:47, Mgr. Michaela Hylsová, Ph.D.

Anotace

V originále

The analysis of microplastics with current spectroscopic and pyrolytic methods is reaching its limits, especially with regard to detailed spatial distribution in biological tissues. This limitation hampers a comprehensive understanding of the effects of microplastics on organisms. Therefore, there is a pressing need to expand the analytical approaches to study microplastics in biota. In this context, the aim of this study was to test the applicability of non-destructive 3D imaging using X-ray micro-computed tomography (microCT) for the detection of microplastics in fish. Zebrafish (Danio rerio) were gavaged with polyethylene spherical microplastics (30-110 mu m) and the distribution of microplastics in the gut was investigated using microCT. The results showed that the particle size distribution determined by microCT closely matched the data from conventional laser diffraction analysis. In addition, microCT was able to detect microplastics in spiked fish tissue and provide precise localization data by tracing particles of known type and shape. MicroCT offers a novel approach for tracking microplastics in organisms and enables accurate sizing without compromising the integrity of the tissue under investigation. It therefore represents a valuable addition to spectroscopic methods, which are widely used for the detection of microplastics based on their chemical composition but do not provide data on their spatial distribution.

Návaznosti

GF23-13617L, projekt VaV

Název: Vliv mikroplastů na modelové organismy a jejich osud v životním prostředí za použití zobrazovacích a spektroskopických technik

Investor: Grantová agentura ČR, Vliv mikroplastů na modelové organismy a jejich osud v životním prostředí za použití zobrazovacích a spektroskopických technik, Partnerská agentura (Slovinsko)

Citovat

PAROBKOVA, Viktoria; Lukas MALECEK; Marek ZEMEK; Gabriela KALCIKOVA; Michaela VYKYPĚLOVÁ; Marcela BUCHTOVÁ; Ondřej ADAMOVSKÝ; Tomas ZIKMUND a Jozef KAISER. Advancing microplastic detection in zebrafish with micro computed tomography: A novel approach to revealing microplastic distribution in organisms. Journal of Hazardous Materials. Amsterdam: Elsevier Science BV., 2025, roč. 488, May 2025, s. 1-12. ISSN 0304-3894. Dostupné z: https://doi.org/10.1016/j.jhazmat.2025.137442.

@article{2483401,
   author = {Parobkova, Viktoria and Malecek, Lukas and Zemek, Marek and Kalcikova, Gabriela and Vykypělová, Michaela and Buchtová, Marcela and Adamovský, Ondřej and Zikmund, Tomas and Kaiser, Jozef},
   article_location = {Amsterdam},
   article_number = {May 2025},
   doi = {https://doi.org/10.1016/j.jhazmat.2025.137442},
   keywords = {Microplastics; X-ray Computed Tomography; Environment; Plastic Pollution; Imaging},
   language = {eng},
   issn = {0304-3894},
   journal = {Journal of Hazardous Materials},
   title = {Advancing microplastic detection in zebrafish with micro computed tomography: A novel approach to revealing microplastic distribution in organisms},
   url = {https://www.sciencedirect.com/science/article/pii/S0304389425003541?via%3Dihub},
   volume = {488},
   year = {2025}
}

TY  - JOUR
ID  - 2483401
AU  - Parobkova, Viktoria - Malecek, Lukas - Zemek, Marek - Kalcikova, Gabriela - Vykypělová, Michaela - Buchtová, Marcela - Adamovský, Ondřej - Zikmund, Tomas - Kaiser, Jozef
PY  - 2025
TI  - Advancing microplastic detection in zebrafish with micro computed tomography: A novel approach to revealing microplastic distribution in organisms
JF  - Journal of Hazardous Materials
VL  - 488
IS  - May 2025
SP  - 1-12
EP  - 1-12
PB  - Elsevier Science BV.
SN  - 03043894
KW  - Microplastics
KW  - X-ray Computed Tomography
KW  - Environment
KW  - Plastic Pollution
KW  - Imaging
UR  - https://www.sciencedirect.com/science/article/pii/S0304389425003541?via%3Dihub
N2  - The analysis of microplastics with current spectroscopic and pyrolytic methods is reaching its limits, especially with regard to detailed spatial distribution in biological tissues. This limitation hampers a comprehensive understanding of the effects of microplastics on organisms. Therefore, there is a pressing need to expand the analytical approaches to study microplastics in biota. In this context, the aim of this study was to test the applicability of non-destructive 3D imaging using X-ray micro-computed tomography (microCT) for the detection of microplastics in fish. Zebrafish (Danio rerio) were gavaged with polyethylene spherical microplastics (30-110 mu m) and the distribution of microplastics in the gut was investigated using microCT. The results showed that the particle size distribution determined by microCT closely matched the data from conventional laser diffraction analysis. In addition, microCT was able to detect microplastics in spiked fish tissue and provide precise localization data by tracing particles of known type and shape. MicroCT offers a novel approach for tracking microplastics in organisms and enables accurate sizing without compromising the integrity of the tissue under investigation. It therefore represents a valuable addition to spectroscopic methods, which are widely used for the detection of microplastics based on their chemical composition but do not provide data on their spatial distribution.
ER  -

PAROBKOVA, Viktoria; Lukas MALECEK; Marek ZEMEK; Gabriela KALCIKOVA; Michaela VYKYPĚLOVÁ; Marcela BUCHTOVÁ; Ondřej ADAMOVSKÝ; Tomas ZIKMUND a Jozef KAISER. Advancing microplastic detection in zebrafish with micro computed tomography: A novel approach to revealing microplastic distribution in organisms. \textit{Journal of Hazardous Materials}. Amsterdam: Elsevier Science BV., 2025, roč.~488, May 2025, s.~1-12. ISSN~0304-3894. Dostupné z: https://doi.org/10.1016/j.jhazmat.2025.137442.

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