From structural polymorphism to structural metamorphosis of the
coat protein of flexuous filamentous potato virus Y

J 2024

From structural polymorphism to structural metamorphosis of the coat protein of flexuous filamentous potato virus Y

KAVCIC, Luka; Andreja KEZAR; Neza KORITNIK; Magda Tusek ZNIDARIC; Tajda KLOBUCAR et al.

Základní údaje

Originální název

From structural polymorphism to structural metamorphosis of the coat protein of flexuous filamentous potato virus Y

Autoři

KAVCIC, Luka; Andreja KEZAR; Neza KORITNIK; Magda Tusek ZNIDARIC; Tajda KLOBUCAR; Ziga VICIC; Franci MERZEL; Ellie HOLDEN; Justin L P BENESCH a Marjetka PODOBNIK

Vydání

COMMUNICATIONS CHEMISTRY, BERLIN, NATURE PORTFOLIO, 2024, 2399-3669

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10400 1.4 Chemical sciences

Stát vydavatele

Německo

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 6.200

Kód RIV

RIV/00216224:90127/24:00139047

Organizační jednotka

CIISB II

DOI

https://doi.org/10.1038/s42004-024-01100-x

UT WoS

001146671600002

EID Scopus

2-s2.0-85182660244

Klíčová slova anglicky

CRYO-EM STRUCTURE; RNA; POTYVIRUS; FLEXIBILITY; RESOLUTION; EXPRESSION; MECHANISM; SEQUENCE; DYNAMICS; MASS

Štítky

CF CRYO, ne MU, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 13. 3. 2025 10:35, Mgr. Eva Dubská

Anotace

V originále

The structural diversity and tunability of the capsid proteins (CPs) of various icosahedral and rod-shaped viruses have been well studied and exploited in the development of smart hybrid nanoparticles. However, the potential of CPs of the wide-spread flexuous filamentous plant viruses remains to be explored. Here, we show that we can control the shape, size, RNA encapsidation ability, symmetry, stability and surface functionalization of nanoparticles through structure-based design of CP from potato virus Y (PVY). We provide high-resolution insight into CP-based self-assemblies, ranging from large polymorphic or monomorphic filaments to smaller annular, cubic or spherical particles. Furthermore, we show that we can prevent CP self-assembly in bacteria by fusion with a cleavable protein, enabling controlled nanoparticle formation in vitro. Understanding the remarkable structural diversity of PVY CP not only provides possibilities for the production of biodegradable nanoparticles, but may also advance future studies of CP's polymorphism in a biological context.

Návaznosti

90127, velká výzkumná infrastruktura

Název: CIISB II

Citovat

KAVCIC, Luka; Andreja KEZAR; Neza KORITNIK; Magda Tusek ZNIDARIC; Tajda KLOBUCAR; Ziga VICIC; Franci MERZEL; Ellie HOLDEN; Justin L P BENESCH a Marjetka PODOBNIK. From structural polymorphism to structural metamorphosis of the coat protein of flexuous filamentous potato virus Y. COMMUNICATIONS CHEMISTRY. BERLIN: NATURE PORTFOLIO, 2024, roč. 7, č. 1, s. 1-19. ISSN 2399-3669. Dostupné z: https://doi.org/10.1038/s42004-024-01100-x.

@article{2483616,
   author = {Kavcic, Luka and Kezar, Andreja and Koritnik, Neza and Znidaric, Magda Tusek and Klobucar, Tajda and Vicic, Ziga and Merzel, Franci and Holden, Ellie and Benesch, Justin L P and Podobnik, Marjetka},
   article_location = {BERLIN},
   article_number = {1},
   doi = {https://doi.org/10.1038/s42004-024-01100-x},
   keywords = {CRYO-EM STRUCTURE; RNA; POTYVIRUS; FLEXIBILITY; RESOLUTION; EXPRESSION; MECHANISM; SEQUENCE; DYNAMICS; MASS},
   language = {eng},
   issn = {2399-3669},
   journal = {COMMUNICATIONS CHEMISTRY},
   title = {From structural polymorphism to structural metamorphosis of the coat protein of flexuous filamentous potato virus Y},
   url = {https://www.nature.com/articles/s42004-024-01100-x},
   volume = {7},
   year = {2024}
}

TY  - JOUR
ID  - 2483616
AU  - Kavcic, Luka - Kezar, Andreja - Koritnik, Neza - Znidaric, Magda Tusek - Klobucar, Tajda - Vicic, Ziga - Merzel, Franci - Holden, Ellie - Benesch, Justin L P - Podobnik, Marjetka
PY  - 2024
TI  - From structural polymorphism to structural metamorphosis of the coat protein of flexuous filamentous potato virus Y
JF  - COMMUNICATIONS CHEMISTRY
VL  - 7
IS  - 1
SP  - 1-19
EP  - 1-19
PB  - NATURE PORTFOLIO
SN  - 23993669
KW  - CRYO-EM STRUCTURE
KW  - RNA
KW  - POTYVIRUS
KW  - FLEXIBILITY
KW  - RESOLUTION
KW  - EXPRESSION
KW  - MECHANISM
KW  - SEQUENCE
KW  - DYNAMICS
KW  - MASS
UR  - https://www.nature.com/articles/s42004-024-01100-x
N2  - The structural diversity and tunability of the capsid proteins (CPs) of various icosahedral and rod-shaped viruses have been well studied and exploited in the development of smart hybrid nanoparticles. However, the potential of CPs of the wide-spread flexuous filamentous plant viruses remains to be explored. Here, we show that we can control the shape, size, RNA encapsidation ability, symmetry, stability and surface functionalization of nanoparticles through structure-based design of CP from potato virus Y (PVY). We provide high-resolution insight into CP-based self-assemblies, ranging from large polymorphic or monomorphic filaments to smaller annular, cubic or spherical particles. Furthermore, we show that we can prevent CP self-assembly in bacteria by fusion with a cleavable protein, enabling controlled nanoparticle formation in vitro. Understanding the remarkable structural diversity of PVY CP not only provides possibilities for the production of biodegradable nanoparticles, but may also advance future studies of CP's polymorphism in a biological context.
ER  -

KAVCIC, Luka; Andreja KEZAR; Neza KORITNIK; Magda Tusek ZNIDARIC; Tajda KLOBUCAR; Ziga VICIC; Franci MERZEL; Ellie HOLDEN; Justin L P BENESCH a Marjetka PODOBNIK. From structural polymorphism to structural metamorphosis of the coat protein of flexuous filamentous potato virus Y. \textit{COMMUNICATIONS CHEMISTRY}. BERLIN: NATURE PORTFOLIO, 2024, roč.~7, č.~1, s.~1-19. ISSN~2399-3669. Dostupné z: https://doi.org/10.1038/s42004-024-01100-x.

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