Advances in gut microbiota functions in inflammatory bowel
disease: Dysbiosis, management, cytotoxicity assessment, and
therapeutic perspectives

J 2025

Advances in gut microbiota functions in inflammatory bowel disease: Dysbiosis, management, cytotoxicity assessment, and therapeutic perspectives

KUSHKEVYCH, Ivan; Michaela DVOŘÁKOVÁ; Dani DORDEVIC; Bozena FUTOMA-KOLOCH; Mario GAJDACS et al.

Základní údaje

Originální název

Advances in gut microbiota functions in inflammatory bowel disease: Dysbiosis, management, cytotoxicity assessment, and therapeutic perspectives

Autoři

KUSHKEVYCH, Ivan; Michaela DVOŘÁKOVÁ; Dani DORDEVIC; Bozena FUTOMA-KOLOCH; Mario GAJDACS; Lamiaa A. AL-MADBOLY a Mohamed Abd EL-SALAM

Vydání

Computational and Structural Biotechnology Journal, Elsevier, 2025, 2001-0370

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10606 Microbiology

Stát vydavatele

Nizozemské království

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 4.100 v roce 2024

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/25:00140853

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

Gut microbiome; Cytotoxicity assays; Inflammatory bowel disease; Microbiota-host interaction; Anaerobic bacteria; Microbial pathogenesis

Štítky

14314010, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 17. 3. 2025 09:16, Mgr. Marie Novosadová Šípková, DiS.

Anotace

V originále

Inflammatory bowel disease (IBD), including Crohn's disease and ulcerative colitis, have become increasingly prevalent across all human generations. Despite advances in diagnosis, effective long-term therapeutic options remain limited, with many patients experiencing recurrent symptoms after treatment. The multifactorial origins of ulcerative colitis are widely recognized, but the intestinal microbiome, particularly bacteria from the Desulfovibrionaceae family, is thought to play a central role in the pathogenesis of the disease. These bacteria contribute significantly to gut microbial functions, yet their cytotoxic and viability characteristics under disease conditions remain poorly understood. Our review provides insights on recent advancements in methodologies for assessing the cytotoxicity and viability of anaerobic intestinal bacteria, with a specific focus on their relevance to gut health and disease. We introduce overview from current literature on modern techniques including flow cytometry, high-throughput screening, and molecular-based assays, highlighting their applications in understanding the role of Desulfovibrionaceae and other gut microbes in IBD pathogenesis. By bridging methodological advancements with functional implications, this review aims to enhance our understanding of gut microbiotahost interactions, which are crucial for maintaining health and preventing disease through immune modulation, where microbiota help regulate immune responses and prevent excessive inflammation; nutrient metabolism, including the breakdown of dietary fibers into short-chain fatty acids that support gut health; and

Návaznosti

MUNI/A/1774/2024, interní kód MU

Název: Podpora výzkumné činnosti studentů Mikrobiologie 5

Investor: Masarykova univerzita, Podpora výzkumné činnosti studentů Mikrobiologie 5

Citovat

KUSHKEVYCH, Ivan; Michaela DVOŘÁKOVÁ; Dani DORDEVIC; Bozena FUTOMA-KOLOCH; Mario GAJDACS; Lamiaa A. AL-MADBOLY a Mohamed Abd EL-SALAM. Advances in gut microbiota functions in inflammatory bowel disease: Dysbiosis, management, cytotoxicity assessment, and therapeutic perspectives. Computational and Structural Biotechnology Journal. Elsevier, 2025, roč. 27, č. 2025, s. 851-868. ISSN 2001-0370. Dostupné z: https://doi.org/10.1016/j.csbj.2025.02.026.

@article{2484646,
   author = {Kushkevych, Ivan and Dvořáková, Michaela and Dordevic, Dani and FutomaandKoloch, Bozena and Gajdacs, Mario and AlandMadboly, Lamiaa A. and ElandSalam, Mohamed Abd},
   article_number = {2025},
   doi = {https://doi.org/10.1016/j.csbj.2025.02.026},
   keywords = {Gut microbiome; Cytotoxicity assays; Inflammatory bowel disease; Microbiota-host interaction; Anaerobic bacteria; Microbial pathogenesis},
   language = {eng},
   issn = {2001-0370},
   journal = {Computational and Structural Biotechnology Journal},
   title = {Advances in gut microbiota functions in inflammatory bowel disease: Dysbiosis, management, cytotoxicity assessment, and therapeutic perspectives},
   url = {https://doi.org/10.1016/j.csbj.2025.02.026},
   volume = {27},
   year = {2025}
}

TY  - JOUR
ID  - 2484646
AU  - Kushkevych, Ivan - Dvořáková, Michaela - Dordevic, Dani - Futoma-Koloch, Bozena - Gajdacs, Mario - Al-Madboly, Lamiaa A. - El-Salam, Mohamed Abd
PY  - 2025
TI  - Advances in gut microbiota functions in inflammatory bowel disease: Dysbiosis, management, cytotoxicity assessment, and therapeutic perspectives
JF  - Computational and Structural Biotechnology Journal
VL  - 27
IS  - 2025
SP  - 851-868
EP  - 851-868
PB  - Elsevier
SN  - 20010370
KW  - Gut microbiome
KW  - Cytotoxicity assays
KW  - Inflammatory bowel disease
KW  - Microbiota-host interaction
KW  - Anaerobic bacteria
KW  - Microbial pathogenesis
UR  - https://doi.org/10.1016/j.csbj.2025.02.026
N2  - Inflammatory bowel disease (IBD), including Crohn's disease and ulcerative colitis, have become increasingly prevalent across all human generations. Despite advances in diagnosis, effective long-term therapeutic options remain limited, with many patients experiencing recurrent symptoms after treatment. The multifactorial origins of ulcerative colitis are widely recognized, but the intestinal microbiome, particularly bacteria from the Desulfovibrionaceae family, is thought to play a central role in the pathogenesis of the disease. These bacteria contribute significantly to gut microbial functions, yet their cytotoxic and viability characteristics under disease conditions remain poorly understood. Our review provides insights on recent advancements in methodologies for assessing the cytotoxicity and viability of anaerobic intestinal bacteria, with a specific focus on their relevance to gut health and disease. We introduce overview from current literature on modern techniques including flow cytometry, high-throughput screening, and molecular-based assays, highlighting their applications in understanding the role of Desulfovibrionaceae and other gut microbes in IBD pathogenesis. By bridging methodological advancements with functional implications, this review aims to enhance our understanding of gut microbiotahost interactions, which are crucial for maintaining health and preventing disease through immune modulation, where microbiota help regulate immune responses and prevent excessive inflammation; nutrient metabolism, including the breakdown of dietary fibers into short-chain fatty acids that support gut health; and
ER  -

KUSHKEVYCH, Ivan; Michaela DVOŘÁKOVÁ; Dani DORDEVIC; Bozena FUTOMA-KOLOCH; Mario GAJDACS; Lamiaa A. AL-MADBOLY a Mohamed Abd EL-SALAM. Advances in gut microbiota functions in inflammatory bowel disease: Dysbiosis, management, cytotoxicity assessment, and therapeutic perspectives. \textit{Computational and Structural Biotechnology Journal}. Elsevier, 2025, roč.~27, č.~2025, s.~851-868. ISSN~2001-0370. Dostupné z: https://doi.org/10.1016/j.csbj.2025.02.026.

Přehled o publikaci